Isolation, identification and pathogenicity patterns of Mucor hiemalis in cultured Cyprinis carpio communis using challenged system

Magray, Aqib Rehman; Ganai, Bashir Ahmad; Ahmad, Fayaz

doi:10.1016/j.aquaculture.2019.734837

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…M. circinelloides was identified firstly from diseased yellow catfish ( P. fulvidraco ) in the natural environment by morphological and molecular analysis (Ke et al ., 2010). M. hiemalis was identified and its pathogenicity patterns was explored in cultured Cyprinis carpio communis using challenged system (Magray et al ., 2019). Similarly, the authors of this study found that M. hiemalis was isolated and identified from seven kinds of fishes ( P. fluviatilis , H. molitrix , T. mossambica , C. auratus , O. mykiss , C. peled and C. muksum ), whereas M. circinelloides was from three kinds of fishes ( O. mykiss , S. (Racoma) davidi and H. molitrix ).…”

Section: Discussionmentioning

confidence: 99%

“…The typical feature of fungal diseases is visible white, grey, brown, red or greenish patches of filamentous mycelium on the body or fins (Iqbal & Sajjad, 2013; Ke et al ., 2010). After colonization, the fungi can spread rapidly and cover most of the fish body, and then lead to the varying degrees of infection in different species of fishes or their egg, such as Micropterus salmoides , Pelteobagrus fulvidraco , Oncorhynchus mykiss , Carassius auratus , Hypophthalmichthys molitrix , Danio rerio , Cyprinus carpio (Fadaeifard, 2011; Huang et al ., 1996; Iqbal et al ., 2012; Ke et al ., 2010; Kulatunga et al ., 2017; Magray et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

“…The pathogens which cause fish fungal diseases are mainly divided into two categories, fungal‐like pathogens oomycete and other mitosporic fungi (Fadaeifard, 2011; Hatai, 2012; Magray et al ., 2019; Yanong, 2003). For a long time, mycotic fungal infections by oomycete widely occur in wild and farmed freshwater fish and their eggs, such as Pythium , Saprolegnia , Achlya and Aphanomyces (Ghiasi et al ., 2010; Hafez, 2016; Khoo, 2000; Lik‐Ming et al ., 2018; Sarowar et al ., 2019; Willoughby, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification and phylogenetic analysis of fungal pathogens isolated from diseased fish in Xinjiang, China

Zhang

Han

et al. 2021

Journal of Fish Biology

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The outbreaks of fungal diseases in cultured fish have been severe in recent years, which is harmful to the healthy and sustainable development of fish farming. In this study, an investigation was conducted for significant fungal infections of 12 species of fish in four regions in Xinjiang, China, to understand the distribution of local fish fungal pathogens. Twenty‐six fungal strains with pathogenicity were isolated, and the challenge experiment showed that eight strains from Changji area had high infection rate to fish eggs. Based on internal transcribed spacer sequence data and molecular analysis, the 26 strains were classified into nine different species of six fungal genera. Phylogenetic analysis showed that all strains were divided into two clades, namely Cluster 1 (contains only the genus Mucor) and Cluster 2 (consists of five small branches), and the distribution of strains from the same region was scattered in two clusters. There is no strict host selectivity for these fungi to infect fish. Mucor sp. are the main fungal pathogen of fish in these four regions, whereas Hypophthalmichthys molitrix and Carassius auratus are two types of fish that were susceptible to pathogen. In addition, the environmental adaptability experiments showed that eight highly pathogenic strains have different adaptability to the environment, and their optimum temperature and pH were 25°C and 7.0, respectively, whereas the concentration of NaCl was negatively correlated with the growth of strains. Therefore, these results indicated that the coinfection of multiple fungal pathogens in a culture region should be considered in the future study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular identification and phylogenetic analysis of fungal pathogens isolated from diseased fish in Xinjiang, China

Zhang

Han

et al. 2021

Journal of Fish Biology

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“…Mucor genus was represented in our study by two species: M. hiemalis and M. circinelloides, which were capable of growing at 37 • C. This trait fulfills the requirements of internal infections in mammals [68]. In fact, M. hiemalis and M. circinelloides can cause mucormycosis disease in immunocompromised humans and animals, which affects the nose, sinuses, eyes, and brain [69][70][71]. Other mucormycosis can spread to the lungs, stomach, intestines, and skin.…”

Section: Discussionmentioning

confidence: 99%

A Culture-Based Study of Micromycetes Isolated from the Urban Nests of Grey Heron (Ardea cinerea) in SW Poland

Ogórek

Borzęcka

Kłosińska

et al. 2022

Animals

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There are many positive relationships between micromycetes and birds: They can spread fungal spores, and fungi facilitate cavity woodpecker excavation by preparing and modifying excavation sites. In turn, bird nests are mainly a source of potentially zoopathogenic fungi. The Wrocław city centre hosts the biggest grey heron breeding colony in Poland with at least 240 breeding birds pairs. To assess the possible public health risks associated with bird nests, the goal of the present study was to identify cultivable fungi present in the nests of grey herons (Ardea cinerea) in Wrocław. Additionally, attempts were made to determine whether the obtained species of fungi may pose a potential threat to animal health. Fungi were cultured at 23 and 37 ± 0.5 °C, and identified based on phenotypic and genotypic traits. Moreover, during routine inspection, visible fungal growth in some of the nests was found. Overall, 10 different fungal species were obtained in the study (Alternaria alternata, Aspergillus fumigatus, Botryotrichum piluliferum, Cladosporium cladosporioides, Epicoccum layuense, Mucor circinelloides, M.hiemalis, Penicillium atramentosum, P.coprophilum, and P.griseofulvum). They are both cosmopolitan species and a source of potential threat to humans, homoiothermous animals and plants. The greatest number of fungal species was obtained from the nest fragments with visible fungal growth incubated at 23 °C, and the least from western conifer seed bugs (Leptoglossus occidentalis) inhabiting the nests. The species such as A. fumigatus, P. coprophilum, and P.griseofulvum can be directly related to the occurrence of visible fungal growth on plant fragments of grey heron’s nests.

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“…Labeo rohita is the most important commercial fish in India with maximum market demand and acceptability as food by consumers due to their taste and flesh. " However, unpredictable mortalities caused by bacterial, fungal, and viral remain a major challenge to the aquaculture industry worldwide and have led to great economic losses in the fish culture industry every year" [3] and [4]. "The common fish pathogen Aeromonas hydrophila outbreaks cause high mortality rates in fish farming by hemorrhagic septicemia (acute form), skin ulcers, and underlying necrosis of the musculature (chronic form)" [5].…”

Section: Introductionmentioning

confidence: 99%

Dietary Probiotic Supplementation on Hematological and Immunological Parameters of Indian Major Carp (Labeo rohita)

Priyadharshini,

Margaret,

Nisha

et al. 2024

UPJOZ

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A 60-day feeding trial was conducted to evaluate the influence of dietary supplementation of probiotic bacteria (Lactobacillus acidophilus) to improve the hematological and immunological parameters of Indian Major carp Rohu (Labeo rohita). The rohu Fingerlings (mean body weight 6.0±0.6g) were randomly distributed into Four treatment groups each having 10 fishes fed with basal diet and a probiotic supplement containing L. acidophilus (1.0×107 CFU/g), then challenged with A. hydrophila (0.1 × 107 CFU/mL) was injected intraperitoneally with 0.1ml concentration of 107 CFU/ml on 55th day. After 72 hours, the blood samples were collected from five fish from each group. The hematological parameters such as hemoglobin (Hb; g/dL), red blood cell (RBC; ×106/mm3), white blood cell (WBC; ×103/mm3), and Packed corpuscular volume (PCV) along with immunological parameters of total immunoglobulin, serum lysozyme activity, and Aminotransferases were examined and compared with the control group. The fish fed with probiotics showed significant improvement in hematological and Immunological Parameters (P≤0.05) as compared to control. This study proved that probiotic bacteria L. acidophilus are resistant to pathogenic A. hydrophila and can contribute to improving the immune system, survival rate, and growth of experimental fish against the fish pathogen A. hydrophila.

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Isolation, identification and pathogenicity patterns of Mucor hiemalis in cultured Cyprinis carpio communis using challenged system

Cited by 6 publications

References 24 publications

Molecular identification and phylogenetic analysis of fungal pathogens isolated from diseased fish in Xinjiang, China

Molecular identification and phylogenetic analysis of fungal pathogens isolated from diseased fish in Xinjiang, China

A Culture-Based Study of Micromycetes Isolated from the Urban Nests of Grey Heron (Ardea cinerea) in SW Poland

Dietary Probiotic Supplementation on Hematological and Immunological Parameters of Indian Major Carp (Labeo rohita)

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