Isolation, Identification and Optimization of a New Extracellular Lipase Producing Strain of Rhizopus sp.

Kantak, Jayshree B.; Bagade, Aditi V.; Mahajan, Siddharth; Pawar, Sharad; Shouche, Yogesh S.; Prabhune, Asmita

doi:10.1007/s12010-011-9188-0

Cited by 16 publications

(12 citation statements)

References 24 publications

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“…Enzyme production with relatively high activity is possible only after optimizing growth parameters. Optimization of the various nutritional and physical parameters is known to significantly increase product yield, as culture environment is known to have a dramatic influence on enzyme production [55]. By providing the suitable cultural conditions, yield of the enzyme can be enhanced several folds.…”

Section: Resultsmentioning

confidence: 99%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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This study investigated the screening, production and optimization of an extracellular lipase from a fungus isolated from the contaminated soil of a palm oil processing shed. This was with a view to obtaining a strain that can secrete lipase with biochemical properties exploitable for biotechnological applications such as bioremediation of oil contaminated sites. Soil samples were collected from palm oil contaminated sites in Gbogan, Osun State, Nigeria (Latitude N 7°29.1481´ and Longitude E 4°20.7587´). The isolated fungal strains were screened on tributyrin agar for exogenous lipolytic activity. Molecular identification was carried out by amplifications of ITS-1, 5.8S and ITS-2 regions. The effects of incubation time, inducers, pH, temperature, carbon and nitrogen sources were varied for optimal lipase production using one factor at a time approach. Rhizopus oryzae ZAC3 (NCBI accession No: KX035094) was identified as the highest lipaseproducing strain. Maximum lipase production was observed on the fourth day, pH 5.0 and a temperature of 45 o C. Olive oil, xylose and yeast extract were the best inducer, carbon and nitrogen sources respectively for lipase production. There was a 2.02 fold increase in lipase production under these optimized conditions. In conclusion, Rhizopus oryzae ZAC3 lipase has properties exploitable for industrial and biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Ayinla¹,

Ademakinwa²,

Agboola³

2017

J App Biol Biotech

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“…They are present in plants and animals, as well as in a number of microorganisms. However, for industrial applications, microbial, that is, bacterial , yeast , and fungal , lipases are mainly exploited because of their easy extraction and their potential for an unlimited modification of culture conditions.…”

Section: Introductionmentioning

confidence: 99%

“…They are present in plants and animals, as well as in a number of microorganisms. However, for industrial applications, microbial, that is, bacterial [1], yeast [2,3], and fungal [4,5], lipases are mainly exploited because of their easy extraction and their potential for an unlimited modification of culture conditions. Because of their widely diversified enzymatic properties, in particular, the ability to perform enantioselective hydrolytic reactions and formation of a wide range of esters and amide bonds, microbial lipases seem to be very attractive for a number of industrial applications [6].…”

Section: Introductionmentioning

confidence: 99%

New alkaline lipase from Rhizomucor variabilis: Biochemical properties and stability in the presence of microbial EPS

Bancerz

Osińska-Jaroszuk

Jaszek

et al. 2015

Biotech and App Biochem

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A new strain of Rhizomucor variabilis producing an active extracellular lipase was identified and characterized in the present studies. The culture conditions were optimized and the highest lipase production amounting to 136 U/mL was achieved after 4 days of cultivation. The optimum pH (5.5) and temperature (28 °C) were determined as the best conditions for R. variabilis lipase production. The isolated enzyme preparation exhibited maximum activity at 40 °C and pH 8.0. Lipase from R. variabilis was stable up to 50 °C during 2 H retaining 80% of its initial activity. The enzyme was highly stable in the pH range of 7.0-9.0. Moreover, the addition of naturally obtained exopolysaccharides (EPS) significantly enhanced lipase activity. The presence of EPS derived from Ganoderma applanatum and Rhizobium leguminosarum enhanced the lipase activity, which was 22% and 31%, respectively, higher than that in the control experiments. Simultaneously, the pH activity profiles remained unchanged. The Michaelis-Menten constant and the turnover number of the enzyme for p-nitrophenyl palmitate in the standard assay conditions were estimated at a level of 0.631 mM and 0.674 Sec(-1) . In conclusion, the results obtained in this work present a newly isolated lipase preparation stabilized with EPS or without modification as a very effective tool for industrial application.

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“…The significance of each coefficient was determined by Student's t ‐test and p ‐values, which are listed in Table . Larger the magnitude of t ‐value and smaller p ‐value, more significant is the corresponding coefficient . This implies that the values of factors having ‘ p ’ values lesser than 0.05 are statistically more significant.…”

Section: Multiple Responses Optimization and Model Buildingmentioning

confidence: 96%

Surface immobilization of Rhizopus oryzae (ATCC 96382) for enhanced production of lipase enzyme by multiple responses optimization

Potumarthi

Liu

Wan

et al. 2012

Asia-Pacific J Chem Eng

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A modified Response Surface Methodology was used for the optimization of lipase production by immobilized cells of Rhizopus oryzae (ATCC 96382). Multiple responses optimization strategy was adopted to optimize process condition of surface immobilization technique for the optimization of lipase productivity. Five parameters, pH, Temperature, revolutions per minute, olive oil and glucose were considered, and their effect was evaluated both qualitatively and quantitatively by using a modified central composite design approach and model validation. The second order regression equation was developed for the three yield parameters are YP/B, YL/P and YL/B, where Y is the yield parameter at their corresponding values of L: Lipase (U/mL), P: Protein (mg/mL) and B: Biomass (mg/mL). The regression equation was fitted and model fit (R2) was 0.604 (YP/B), 0.746 (YL/P) and 0.618 (YL/B), respectively showing an effective fit. Further, the model was validated theoretically and experimentally at higher level of production. Different growth patterns were observed during 120 h of fermentation period. © 2012 Curtin University of Technology and John Wiley & Sons, Ltd.

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Isolation, Identification and Optimization of a New Extracellular Lipase Producing Strain of Rhizopus sp.

Cited by 16 publications

References 24 publications

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

Studies on the Optimization of Lipase Production by Rhizopus sp. ZAC3 Isolated from the Contaminated Soil of a Palm Oil Processing Shed

New alkaline lipase from Rhizomucor variabilis: Biochemical properties and stability in the presence of microbial EPS

Surface immobilization of Rhizopus oryzae (ATCC 96382) for enhanced production of lipase enzyme by multiple responses optimization

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