Isolation, Identification and Genetic Characterisation of a Microsporidium Isolated from Carob Moth,<i>Ectomyelois ceratoniae</i>(Zeller) (Lepidoptera: Pyralidae)

Lloyd, Melissa

doi:10.4001/003.025.0529

Cited by 2 publications

(1 citation statement)

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“…In South Africa, Lloyd and friends reported the second microsporidiosis from the A. ceratoniae in 2017. Although this study mentioned the presence of microsporidial spores with similar size and morphological characteristics as those reported previously by Lange, there was no data or figure in this study put forward to demonstrate this.…”

Section: Discussioncontrasting

confidence: 49%

A new isolate ofNosema fumiferanae(Microsporidia: Nosematidae) from the date mothApomyelois(Ectomyelois)ceratoniae, Zeller, 1839 (Lepidoptera: Pyralidae)

Tosun¹

2020

Parasitology

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In this study, a microsporidian pathogen of the date moth (Apomyelois (Ectomyelois) ceratoniae, Zeller, 1839) also known as the carob moth, is described based on light microscopy, ultrastructural characteristics and comparative molecular analysis. The pathogen infects the gut and hemolymph of A. ceratoniae. All development stages are in direct contact with the host cell cytoplasm. Fresh spores with nuclei arranged in a diplokaryon are oval and measured 3.29 ± 0.23 μm (4.18–3.03 μm, n = 200) in length and 1.91 ± 0.23 μm (2.98–1.66 μm, n = 200) in width. Spores stained with Giemsa's stain measured 3.11 ± 0.31 μm (3.72–2.41 μm, n = 150) in length and 1.76 ± 0.23 μm (2.16–1.25 μm, n = 150) in width. Spores have an isofilar polar filament with 10-12 coils. An 1110 bp long alignment of the current microsporidium showed an SSU rRNA gene difference of only 0.0009, corresponding to >99.91% sequence similarity with Nosema fumiferanae, while RPB1 gene sequences were 98.03% similar within an alignment of 969 bp. All morphological, ultrastructural and molecular features indicate that the microsporidian pathogen of A. ceratoniae is the new isolate of the N. fumiferanae and is named here as Nosema fumiferanae TY61.

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Section: Discussioncontrasting

confidence: 49%