Isolation, Identification and comparatative analysis of SDA and DTM for dermatophytes from clinical samples in a tertiary care hospital

Nasimuddin, Sowmya; Appalaraju, B; P, Surendran; CR, Srinivas

doi:10.9790/0853-131126873

Cited by 8 publications

(8 citation statements)

References 2 publications

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“…Nasimuddin et al . [26] found that only 34.35% of the 300 skin scrapings processed for mycology were positive by direct examination while 49.0% were culture positive. Furthermore, it is not possible to identify a fungus up to species level by this method [17].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and distribution of dermatophytosis lesions on cattle in Plateau State, Nigeria

et al. 2019

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Background and Aim:Dermatophytosis is an infection of the superficial, keratinized structures of the skin, nails, and hair of man and animals caused by a group of fungi called dermatophytes in the genera Trichophyton, Microsporum, and Epidermophyton. The prevalence of dermatophytosis among cattle in Nigeria and Plateau State, in particular, is yet to be fully determined. This study aimed to determine the prevalence and the distribution of dermatophytosis lesions on cattle in Plateau State, Nigeria.Materials and Methods:Four hundred and thirty-seven cattle showing visible skin lesions suggestive of dermatophytosis were drawn from nine local government areas (three each) from the three senatorial districts of Plateau State, Nigeria. Skin scrapings were aseptically collected using a cross-sectional study, in which sampling units were selected using purposive sampling method. Samples were processed for both direct microscopic examination and isolation of dermatophytes in culture. The isolates were stained with lactophenol cotton blue and identified microscopically based on the size, shape, and arrangement of macro- and micro-conidia. The dermatophytes were further identified by determining the sequences of the internal transcribed spacer regions of their ribosomal DNA. Data were analyzed and presented as percentages, bar graph, and Chi-square test of association. p≤0.05 was considered statistically significant.Results:The overall prevalence rate of bovine dermatophytosis in Plateau State was found to be 11.0%. Trichophyton verrucosum was more frequently isolated (54.2%) than Trichophyton mentagrophytes (45.8%). Age, breed, management practice, and season were significantly associated with the occurrence of the disease (p<0.05).Conclusion:Dermatophytosis among cattle may be of public health significance in Plateau State, Nigeria. This is the first report on the prevalence and distribution of dermatophytosis lesions on cattle from Plateau State, Nigeria.

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Section: Discussionmentioning

confidence: 99%

Prevalence and distribution of dermatophytosis lesions on cattle in Plateau State, Nigeria

et al. 2019

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“…A study by Nasimuddin et al reported 49% culture positivity, out of which 14.7% were negative by KOH mount. 22 Mahale et al 7 gave explanation that it may be due to the inactive sporulating phase of the fungi which is difficult to be seen by microscopy. They concluded that findings of direct microscopy by KOH also depend on the skill of the observer.…”

Section: Discussionmentioning

confidence: 99%

Clinicomycological study of tinea infections in and around Pune

et al. 2019

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Background: Tinea is a common fungal infection seen in the tropical and subtropical countries affecting the skin and its appendages. The presentation may vary from mild scaling to severe inflammation with bacterial super infection. It may be confused with other manifestation such as psoriasis, seborrhea, drug eruptions, eczema, and contact dermatitis. Hence correct diagnosis is necessary for appropriate treatment, which will reduce morbidity, discomfort and lessens possibility of transmissions. The aims and objectives were to determine clinicomycological profile of Tinea infections in patients attending dermatology OPD of B. J. Govt. Medical College and Sassoon General Hospital, Pune. Methods: Skin scrapings, nail clippings; hair samples from clinically suspected cases of tinea were collected. Identification of dermatophytes from these samples was done by conventional technique. Results: 119 clinically suspected cases of Tinea infections were processed over a period of one year. Out of these cases mixed infection of Tinea cruris with corporis was the predominant (27.73%) clinical presentation. Among all the samples, fungal filaments were seen by KOH mount in 48 (40.33%) whereas 35 (29.41%) samples were confirmed as dermatophytes by culture. Among these 35 isolates of dermatophytes 20 were T. rubrum, 7 isolates were T. tonsurans. 8 isolates were of T. mentagrophytes. Conclusions: In present study mixed infections of tinea cruris with corporis was the predominant clinical presentation and T. rubrum was the most common dermatophyte isolated.

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“…Ose dicelupkan di dalam alkohol dan dibakar pada api bunsen kemudian didiamkan sesaat. Selanjutnya ose digoreskan pada koloni fungi dermatofita yang tumbuh pada media SDA kemudian diusapkan pada gelas objek yang sebelumnya telah ditetesi LPCB dan ditutup dengan gelas penutup, kemudian diperiksa menggunakan mikroskop cahaya terhadap adanya hifa, makrokonidia, dan mikrokonidia (Nasimuddin et al, 2014;El-ashmawy et al, 2015). Data yang diperoleh dianalisis secara deskriptif.…”

Section: Identifikasi Spesies Dermatofitaunclassified

Identifikasi Spesies Fungi Microsporum gypseum dan M. nanum Penyebab Ringworm pada Sapi Bali (IDENTIFICATION OF SPECIES FUNGI MICROSPORUM GYPSEUM

Putriningsih¹,

Arjentinia²

2018

JVet

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Ringworm is an zoonotic infectious skin disease that can infect many types of animals. This disease is caused by dermatophytes fungi. Ringworm cases in cattle were quite widely reported in various countries but a report about the dermatophytes fungi that caused ringworm in bali cattle have never been published. It is very important in the efforts to give more effective therapies. This study aimed to identify the species of dermatophytes fungi that caused ringworm in bali cattle. Eight bali cattles suspected ringworm samples were taken using superficial skin scraping and trichogram (hair pluck) technique. The samples of skin scrapings dan hair on the area of the lesion were taken for direct microscopic examination to find the presence of fungal elements (hyphae or arthrospora). They were dropped with 10% KOH, were allowed for 10-15 minutes, then were observed using microscope. Skin scrapings dan hair samples that showed positive results were cultured on Sabauroud’s Dextrose Agar (SDA) medium for 1-3 weeks dan were identified using Lactophenol Cotton Blue with microscope. The data were analyzed descriptively. Dermatophytes fungi that isolated dan identified were Microsporum gypseum (75% or 6/8) and Microsporum nanum (25% or 2/8). Both of these fungi have ectothrix invasion/infection tipe, which is the forming of arthrospores/arthroconidia is only on the surface/superficial hair shaft therefore the topical therapy is sufficient to resolve the infection.

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Isolation, Identification and comparatative analysis of SDA and DTM for dermatophytes from clinical samples in a tertiary care hospital

Cited by 8 publications

References 2 publications

Prevalence and distribution of dermatophytosis lesions on cattle in Plateau State, Nigeria

Prevalence and distribution of dermatophytosis lesions on cattle in Plateau State, Nigeria

Clinicomycological study of tinea infections in and around Pune

Identifikasi Spesies Fungi Microsporum gypseum dan M. nanum Penyebab Ringworm pada Sapi Bali (IDENTIFICATION OF SPECIES FUNGI MICROSPORUM GYPSEUM

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