Isolation, identification, and classification of a novel rhabdovirus from diseased Chinese rice-field eels (Monopterus albus)

Liu, Wenzhi; Fan, Yuding; Li, Zhong; Zhao, Jianqing; Zhou, Yong; Jiang, Nan; Zeng, Jia; Cain, Kenneth D.; Zeng, Lingbing

doi:10.1007/s00705-018-4054-9

Cited by 19 publications

(26 citation statements)

References 28 publications

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“…Tissue samples of liver, spleen, kidney, digestive tract, gill, heart and brain from diseased tilapia were fixed in 4% paraformaldehyde (PFA) for 24 hours at 4°C, and washed with Dulbecco’s phosphate-buffered saline (DPBS, Sigma, USA), dehydrated with 30% sucrose/DPBS, and embedded in optimum cutting temperature compound (OCT). Samples were cut (8 μm thick) at -20°C with cryostat (CM1950, Leica, Germany), stained with Hematoxylin-Eosin (HE) and examined by light microscopy with CCD picture system (DM2500, Leica, Germany) [ 29 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…The 5′ and 3′ regions of the NS of parvovirus in the diseased tilapia was identified by performing rapid amplification of cDNA ends (RACE) PCR with the Clontech SMART cDNA synthesis kit (TaKaRa, Japan). To extend the 5′ region of the cDNA sequence, 5′ RACE was performed using a gene-specific primer (TiPV-rR1–rR2; Table 1 ) [ 30 ]. 3′ RACE was performed using an oligo (dT) adaptor primer with the TaKaRa RNA PCR Kit (TaKaRa, Japan) and was directly used as a template for two round PCR detection with the F2027 for NS1 or F3912 for VP1 and a reverse adaptor primer (TiPV-rF1–rF2; Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Amplified products were analyzed by electrophoresis using a 1.5% agarose gels and ethidium bromide staining. The expected PCR products at 534bp were excised, purified and sequenced as previously described [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

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Determination of a novel parvovirus pathogen associated with massive mortality in adult tilapia

Liu

Zhang

et al. 2020

PLoS Pathog

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Tilapia is one of the most important economic and fastest-growing species in aquaculture worldwide. In 2015, an epidemic associated with severe mortality occurred in adult tilapia in Hubei, China. The causative pathogen was identified as Tilapia parvovirus (TiPV) by virus isolation, electron microscopy, experimental challenge, In situ hybridization (ISH), indirect immunofluorescence (IFA), and viral gene sequencing. Electron microscopy revealed large numbers of parvovirus particles in the organs of diseased fish, including kidney, spleen, liver, heart, brain, gill, intestine, etc. The virions were spherical in shape, non-enveloped and approximately 30nm in diameter. The TiPV was isolated and propagated in tilapia brain cells (TiB) and induced a typical cytopathic effect (CPE) after 3 days post-infection (dpi). This virus was used to experimentally infect adult tilapia and clinical disease symptoms similar to those observed naturally were replicated. Additionally, the results of ISH and IFA showed positive signals in kidney and spleen tissues from TiPV-infected fish. To identify TiPV-specific sequences, the near complete genome of TiPV was obtained and determined to be 4269 bp in size. Phylogenetic analysis of the NS1 sequence revealed that TiPV is a novel parvovirus, forms a separate branch in proposed genus Chapparvovirus of Parvoviridae . Results presented here confirm that TiPV is a novel parvovirus pathogen that can cause massive mortality in adult tilapia. This provides a basis for the further studies to define the epidemiology, pathology, diagnosis, prevention and treatment of this emerging viral disease.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Determination of a novel parvovirus pathogen associated with massive mortality in adult tilapia

Liu

Zhang

et al. 2020

PLoS Pathog

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“…At present, though artificial propagation of rice field eel has been successfully practised, its larvae for artificial culture have been mainly supplied from wild resource (Guan, Zhou, Cui, & Feng, 1996). During the intensive cage‐culture conditions, harmful diseases from parasitic, bacterial and viral infections could often result in great economic losses of aquaculture producers (Liu et al., 2019; Shao et al., 2016; Xia et al., 2019). It is necessary that high‐quality breeds would be screened or cultivated to meet the aquaculture producers’ demands.…”

Section: Discussionmentioning

confidence: 99%

“…It is therefore concluded that the three strains possess significant differences at growth rate, fecundity and nutritive composition. Up to now, studies on the rice field eel have been mainly focused on molecular mechanisms of sex reverse (Cai et al., 2017; Gao, Dan, Hu, & Li, 2016; He et al., 2014; Sheng et al., 2015), population genetics (Cai et al., 2008; Li, Sun, Fan, & Zhang, 2013; Liang, Guo, Li, Luo, & Zou, 2016), disease (Liu et al., 2019; Shao, Yuan, Shen, Hu, & Gu, 2016; Xia et al., 2019) and immune genes (Gao et al., 2019; Tang et al., 2019; Xu et al., 2016). Limited information of molecular mechanism, however, was available on the differences at growth rate, fecundity and nutritive composition among the three strains with different body colours.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis of livers from three strains of Chinese swamp eels

et al. 2020

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Swamp eel or rice field eel (Monopterus albus) is one of the most economically valuable freshwater fishes in China. Based on the body colour patterns, Chinese rice field eels are commonly classified into three strains, including deep‐yellow strain (DY), pale‐yellow strain (PY) and steel‐grey strain (SG). Reports have shown that DY strain is superior to the other two strains at growth rate and fecundity. In the present study, RNA‐Seq technology was employed to profile liver transcriptomes of these three strains, and differentially expressed genes (DEGs) were identified. The hierarchical clustering analysis showed that expression patterns of liver transcripts among the three strains were different. A total of 3,296 DEGs, 3,043 DEGs and 143 DEGs were identified, respectively, from DY versus SG, DY versus PY and PY versus SG. The three comparison groups shared three DEGs, of which one DEG (batf) played a vital role in immune system. Functional enrichment analyses of DEGs in DY versus PY and DY versus SG revealed that significantly enriched Gene Ontology terms and KEGG pathways were mainly involved in innate immune responses and lipid metabolism. Additionally, most of DEGs related to immunity were mainly up‐regulated in DY compared to PY and SG. The above results indicated that the DY strain might have strong adaptability and resistance to disease, which might support their superiority for better growth and reproduction.

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2022 taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales

et al. 2022

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In March 2022, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by two new families (bunyaviral Discoviridae and Tulasviridae), 41 new genera, and 98 new species. Three hundred forty-nine species were renamed and/or moved. The accidentally misspelled names of seven species were corrected. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.

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Isolation, identification, and classification of a novel rhabdovirus from diseased Chinese rice-field eels (Monopterus albus)

Cited by 19 publications

References 28 publications

Determination of a novel parvovirus pathogen associated with massive mortality in adult tilapia

Determination of a novel parvovirus pathogen associated with massive mortality in adult tilapia

Comparative transcriptome analysis of livers from three strains of Chinese swamp eels

2022 taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales

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