Isolation, identification and characterization of Paenibacillus polymyxa CR1 with potentials for biopesticide, biofertilization, biomass degradation and biofuel production

Abstract

Background Paenibacillus polymyxa is a plant-growth promoting rhizobacterium that could be exploited as an environmentally friendlier alternative to chemical fertilizers and pesticides. Various strains have been isolated that can benefit agriculture through antimicrobial activity, nitrogen fixation, phosphate solubilization, plant hormone production, or lignocellulose degradation. However, no single strain has yet been identified in which all of these advantageous traits have been confirmed.Results P. polymyxa CR1 was isolated from degrading corn roots from southern Ontario, Canada. It was shown to possess in vitro antagonistic activities against the common plant pathogens Phytophthora sojae P6497 (oomycete), Rhizoctonia solani 1809 (basidiomycete fungus), Cylindrocarpon destructans 2062 (ascomycete fungus), Pseudomonas syringae DC3000 (bacterium), and Xanthomonas campestris 93-1 (bacterium), as well as Bacillus cereus (bacterium), an agent of food-borne illness. P. polymyxa CR1 enhanced growth of maize, potato, cucumber, Arabidopsis, and tomato plants; utilized atmospheric nitrogen and insoluble phosphorus; produced the phytohormone indole-3-acetic acid (IAA); and degraded and utilized the major components of lignocellulose (lignin, cellulose, and hemicellulose).Conclusions P. polymyxa CR1 has multiple beneficial traits that are relevant to sustainable agriculture and the bio-economy. This strain could be developed for field application in order to control pathogens, promote plant growth, and degrade crop residues after harvest.

Current knowledge and perspectives of Paenibacillus: a review

“…Like other plant growth-promoting bacteria, they accomplish this through various facets. Plant-associated species of Paenibacillus can directly influence plant growth by producing indole-3-acetic acid (IAA) and other auxin phytohormones, solubilizing inaccessible phosphorous into form that can be taken up by plant roots, and some species can also fix atmospheric nitrogen [18]. In addition, Paenibacillus helps to control phytopathogens by triggering induced systemic resistance (ISR) and/or producing a variety of biocidal substances (see “Biocontrol” and “Antimicrobial peptides” sections).…”

Inhibition of Klebsiella pneumonia by a Novel Strain of Paenibacillus

“…Fortunately, we isolated a strain of Paenibacillus from the feces of the healthy dogs in the kennel with the outbreak of Klebsiella infection, and the Paenibacillus strain demonstrated potent activity against the K. pneumoniae pathogen. P. polymyxa is well known for its ability to produce a variety of metabolites with antimicrobial activities(34–36). By combining transposon mutagenesis and bioinformatics analysis, we located the gene cluster involved in the antagonism.…”

Local decomposer increase composting rate and produce quality rice straw compost

“…Pseudomonas fluorescens is one of the bacteria that has the ability to dissolve phosphate (Soesanto et al, 2011;Alemu, 2013) and is also ideal as a biofertilizer (Oteino et al, 2015). There are also Paenibacillus polymyxa bacteria which in addition have the ability to degrade lignocellulose also have the ability to dissolve phosphate and nitrogen fixation (Weselowski et al, 2016). Aspergillus niger is one of the fungi have the ability to provide phosphorus for plants (Salih et al, 1989).…”