Isolation, identification and characterization of Shewanella algae from reared tongue sole, Cynoglossus semilaevis Günther

Han, Zhuoran; Sun, Jingfeng; Lv, Aijun; Sung, Yeong Yik; Shi, Hongyue; Hu, Xiucai; Xing, Kun

doi:10.1016/j.aquaculture.2016.10.038

Cited by 51 publications

(32 citation statements)

References 21 publications

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“…Deaths occurring at 12 h after challenge indicated that the infection caused by this pathogen had a short incubation period. Similar phenomena in tongue soles infected with a pathogenic Shewanella algae were reported by Han et al (2017), who noted that the short incubation period resulted in rapid disease progression.…”

Section: Discussionsupporting

confidence: 83%

“…A BLAST search for sequences was carried out via the NCBI website. Phylogenetic trees were established using the neighborjoining method in the MEGA 5.1 software package following Han et al (2017).…”

Section: S Rrna and Gyrb Gene Sequences Analysismentioning

confidence: 99%

“…Growing characteristics were explored according to a previous study (Han et al, 2017). The impact of pH on growth was investigated within a 3-11 value range in LB broth at 37 • C; the effect of temperature was assessed at 27 • C, 32 • C, 37 • C and 42 • C in LB broth at pH 7.…”

Section: Growing Characteristicsmentioning

confidence: 99%

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Isolation, Identification and Characteristics of Aeromonas veronii From Diseased Crucian Carp (Carassius auratus gibelio)

et al. 2019

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Aeromonas species often cause disease in farmed fish. In the present study, dominant bacteria were isolated from diseased crucian carp (Carassius auratus gibelio). Based on this, a bacterial isolate was tentatively named CFJY-623. This isolate was identified as Aeromonas veronii based on analysis of its morphological, physiological, and biochemical features, as well as 16S rRNA and gyrB gene sequences. Six virulence genes related to pathogenicity including aerolysin, cytotonic enterotoxins, elastase, glycerophospholipid: cholesterol acyltransferase, lipase, and serine protease were identified in this A. veronii isolate. The median lethal dosage (LD50) of the CFJY-623 isolate for crucian carp was determined as 1.31 × 10 7 CFU/mL. Artificial experimental infection showed that the CFJY-623 isolate caused considerable histological lesions in the fish, including tissue cell degeneration, necrosis, and inflammatory cell infiltrating. Drug sensitivity testing showed that the isolate was susceptible to aminoglycosides, carbapenemes, and nitrofurans. Exploring its growing features showed that this isolate exhibited a high level of environmental adaptability. These results provided a scientific basis for the identification of A. veronii and treatment for fish infected by this pathogen.

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Section: Discussionsupporting

confidence: 83%

Section: S Rrna and Gyrb Gene Sequences Analysismentioning

confidence: 99%

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Isolation, Identification and Characteristics of Aeromonas veronii From Diseased Crucian Carp (Carassius auratus gibelio)

et al. 2019

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“…The PCR reaction of the samples underwent an initial denaturation of 4 min at 95 °C, and then 30 cycles of 45 s at 94 °C, 45 s at 55 °C and 1 min at 72 °C, followed by 10 min at 72 °C. Reaction products were purified and cloned according to the report of Han et al (2017). The nucleotide sequences were compared with known sequences in the NCBI database by using the Blast tool (NCBI, http://www.ncbi.nih.gov/BLAST /).…”

Section: Genotypic Identificationmentioning

confidence: 99%

“…Growth characteristics were tested according to the method of Han et al (2017). The temperature, pH value and NaCl concentration were adjusted to various conditions based on LB broth (Beijing Aoboxing Bio-Technology Co., Ltd., Beijing, China).…”

Section: Growth Characteristicsmentioning

confidence: 99%

A candidate probiotic strain of Enterococcus faecium from the intestine of the crucian carp Carassius auratus

Mao

Sun

et al. 2020

AMB Expr

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In the present study, a Gram-positive bacterium was isolated from the intestine of healthy crucian carp Carassius auratus and named strain R8. It was initially identified as Enterococcus faecium according to its morphological, physiological and biochemical characteristics. Further identification by using 16S rRNA gene sequence analysis confirmed the R8 strain (Genbank accession no. MF928076) as E. faecium. Challenge and hemolysis experiments showed that the E. faecium R8 strain had no toxicity to the crucian carp. Bacteriostatic experiment showed that this isolate obviously inhibited the growth of Aeromonas veronii and Staphylococcus haemolyticus. The proliferation of E. faecium R8 strain occurred after exposure to various growth conditions such as at pH values from 2.0 to 4.0 for 8 h, bile concentrations from 0.2 to 1.2% and high temperature of 80 °C. This bacterial strain grew best under the condition of 37 °C, pH 7.0 and salinity 30 ppt, and its growth curve exhibited four distinct phases. These results showed that the E. faecium R8 strain had potential probiotic characteristics and could be used as a candidate strain for aquatic probiotics.

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Characterization of Pathogenic Pseudomonas alcaligenes Isolated from Koi Carp in China

Bai

Huo

et al. 2021

J Aqua Anim Hlth

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Pseudomonas alcaligenes infection is rare in aquaculture. In this study, we provide the first report on the characterization of P. alcaligenes from koi (a variant of Common Carp Cyprinus carpio) in China. A gram‐negative bacterium was isolated from the diseased koi and was named KCP‐516. Morphological and biochemical tests as well as phylogenetic tree analyses derived from 16S ribosomal RNA, gyrase subunit A, and gyrase subunit B gene sequencing all strongly indicated that the isolate KCP‐516 was P. alcaligenes. In liquid medium, the optimal growth conditions were 25°C, 2.5% NaCl, and pH 8. The pathogenicity of the isolate was demonstrated in koi, with 7.0 × 104 CFU/g fish weight identified as the dose lethal to 50% of test fish. The results will provide a scientific reference for the diagnosis and treatment of P. alcaligenes infection.

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Isolation, identification and characterization of Shewanella algae from reared tongue sole, Cynoglossus semilaevis Günther

Cited by 51 publications

References 21 publications

Isolation, Identification and Characteristics of Aeromonas veronii From Diseased Crucian Carp (Carassius auratus gibelio)

Isolation, Identification and Characteristics of Aeromonas veronii From Diseased Crucian Carp (Carassius auratus gibelio)

A candidate probiotic strain of Enterococcus faecium from the intestine of the crucian carp Carassius auratus

Characterization of Pathogenic Pseudomonas alcaligenes Isolated from Koi Carp in China

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