Isolation, identification and characterization of Bacillus subtilis ZJB-063, a versatile nitrile-converting bacterium

Zheng, Yu‐Guo; Chen, Jing; Liu, Zhi‐Qiang; Wu, Minghuo; Xing, Liang-Ying; Shen, Yin‐Chu

doi:10.1007/s00253-007-1236-x

Cited by 66 publications

(39 citation statements)

References 29 publications

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“…Our experiments also indicated inducible nature of nitrile-hydrolysing activity like many nitrilases reported in literature [9]. However, there are also reports on the constitutive nature of nitrilases as observed in Bacillus subtilis [2] and in Rhodococcus ruber [7]. Choice of inducer may affect the substrate specificity of nitrilase [16,17].…”

Section: Selection and Identification Of A Nitrile-utilizing Strainsupporting

confidence: 75%

“…Nitriles are toxic in nature, but can be hydrolysed to corresponding less-toxic and valuable carboxylic acids either by nitrilase (EC 3.5.5.1), or by nitrile-hydratase (EC 4.2.1.84) and amidase (EC 3.5.1.4). Nitrilases have been reported in a variety of microbes including Alcaligenes [1], Bacillus [2], Burkholderia [3], Labrenzia [4], Pseudomonas [5], Pyrococcus [6] and Rhodococcus [7]. In spite of many nitrilases reported in literature, the quest for their new sources has never ended.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Characterization of a Nitrile-Hydrolysing Bacterium Isoptericola variabilis RGT01

et al. 2014

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A nitrile-hydrolysing bacterium, identified as Isoptericola variabilis RGT01, was isolated from industrial effluent through enrichment culture technique using acrylonitrile as the carbon source. Whole cells of this microorganism exhibited a broad range of nitrile-hydrolysing activity as they hydrolysed five aliphatic nitriles (acetonitrile, acrylonitrile, propionitrile, butyronitrile and valeronitrile), two aromatic nitriles (benzonitrile and m-Tolunitrile) and two arylacetonitriles (4-Methoxyphenyl acetonitrile and phenoxyacetonitrile). The nitrile-hydrolysing activity was inducible in nature and acetonitrile proved to be the most efficient inducer. Minimal salt medium supplemented with 50 mM acetonitrile, an incubation temperature of 30°C with 2 % v/v inoculum, at 200 rpm and incubation of 48 h were found to be the optimal conditions for maximum production (2.64 ± 0.12 U/mg) of nitrile-hydrolysing activity. This activity was stable at 30°C as it retained around 86 % activity after 4 h at this temperature, but was thermolabile with a half-life of 120 min and 45 min at 40°C and 50°C respectively.

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Section: Selection and Identification Of A Nitrile-utilizing Strainsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Nitrile-Hydrolysing Bacterium Isoptericola variabilis RGT01

et al. 2014

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“…The carbon-source utilization was examined by a standardized micromethod, employing with the Biolog microstation (GP2; Biolog Hwyward, USA). 16S rDNA identification was performed according to Zheng et al (2008).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Multiple-stress Tolerance Tetragenococcus halophilus and Application as Starter Culture in Chinese Horsebean-Chili-Paste Manufacture for Quality Improvement

Liu

et al. 2013

FSTR

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The aim of this study was to investigate the effect of Tetragenococcus halophilus addition on the quality of Chinese Horsebean-Chili-Paste (CHCP). T. halophilus SZ-B-2 isolated from soy sauce moromi was identified based on physiological tests, carbon substrate utilization patterns (Biolog) and the 16S rDNA sequence and deposited as T. halophilus CGMCC 3792. The strain SZ-B-2 can grow at 25% NaCl and exhibited relatively broad ranges of pH. The potential of T. halophilus as a starter culture for quality improvement in CHCP fermentation was elucidated. As a result, T. halophilus inoculated sample exhibited higher total titratable acid, formol nitrogen and reducing sugar contents compared with the control. In addition, the addition of T. halophilus decreased the content of nitrite by 39.4%. Major volatile compounds were 9,12-octadecadienoic acid, 1,2-propanediol, ethyl 9,12-octadecadienoate, ethyl 9-hexadecenoate. The inoculated sample showed higher contents of volatile compounds including acids, alcohols, esters and aromatic compounds. Thus, T. halophilus addition may promote the formation of desirable odor and improve the flavor of CHCP.Keywords: Tetragenococcus halophilus, Chinese horsebean-chili-paste, nitrite, volatile compounds *To whom correspondence should be addressed. E-mail: zhourqing@scu.edu.cn IntroductionChinese horsebean-chili-paste (CHCP, also called as Doubanjiang) is a traditionally fermented condiment. The manufacture process of CHCP includes three phases: koji manufacturing, horsebean grain as well as smashed chili fermentation and ripening of horsebean-chili mixture. For the first phase, the shelled horsebean was soaked in boiled water for less than 3 min, and then the horsebean was cooled and mixed with raw wheat powder (horse bean : raw wheat powder 4:1, w/w) to make koji. In the second phase, the koji was mixed with brine (1:1) to create a mixture known as horsebean grain, and the horsebean grain fermentation was conducted for more than 8 months under room temperature. The fresh chili was chopped and mixed with salt (13 − 15% of chili weight) and subsequently, fermented for more than 3 months at ambient temperature to yield chili moromi. In the third phase, horsebean grain and chili moromi were mixed at the ratio of 3:7 (called as initial Jiang pei or paste), and the mixture was ripened in exposure environment at day and light for more than 18 months. The distinct technique during the whole manufacture process contributes to the particular flavor, and the CHCP is recognized as "the soul of Sichuan dishes". Recently, CHCP is gaining increasing interest due to its characteristic flavor and promising market. Traditionally, the production of CHCP solely relies on the natural fermentation process, which leads to the control of the physiochemical and flavor quality rather limited. In addition, the long period of fermentation also limited the development of CHCP industries. The use of starter culture has been reported to improve the quality of many fermented foods as well as shorten the fermentatio...

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“…Methods employed in isolating antagonistic organisms were the soil plate method (Warcup, 1950) and soil dilution plate method (Tuite, 1969). Identifications were performed by using the keys provided by Rifai (1969) and Bissett (1991) for the Trichoderma species, and B. subtilis was identified using Zheng et al (2008) method.…”

Section: Isolation Of F Verticillioides and Antagonistic Organismsmentioning

confidence: 99%

Evaluation of efficacy of some botanicals and bioagents against stalk and ear rot pathogen of maize, Fusarium verticillioides

Akinbode¹,

Ikotun²,

Odebode³

et al. 2014

Afr. J. Microbiol. Res.

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The efficacy of some botanicals and bio-agents were assessed in vitro as the potential biofungicides against Fusarium verticillioides, the causal organism of stalk rot disease of maize plant. The botanicals used in this study were Morinda lucida (Oruwo) and Tithonia diversifolia (Mexican Sunflower) and the bio-agents used were Trichoderma viride, Trichoderma harzianum, Trichoderma pseudokoningii and Bacillius subtilis. All the plant extracts and antagonistic organisms significantly inhibited the growth of Fusarium verticillioides. However, out of the antagonists, T. viride provided the highest inhibitory effect followed by T. pseudokoningii, T. harzianum, and B. subtilis which was the lowest suppressive antagonist. The extract from T. diversifolia, provided the highest inhibitory effect followed by M. lucida.

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Isolation, identification and characterization of Bacillus subtilis ZJB-063, a versatile nitrile-converting bacterium

Cited by 66 publications

References 29 publications

Isolation and Characterization of a Nitrile-Hydrolysing Bacterium Isoptericola variabilis RGT01

Isolation and Characterization of a Nitrile-Hydrolysing Bacterium Isoptericola variabilis RGT01

Characterization of a Multiple-stress Tolerance Tetragenococcus halophilus and Application as Starter Culture in Chinese Horsebean-Chili-Paste Manufacture for Quality Improvement

Evaluation of efficacy of some botanicals and bioagents against stalk and ear rot pathogen of maize, Fusarium verticillioides

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