Isolation, identification and characterization of four cellulolytic actinomycetes and their cellulases

Abstract

Four highly cellulolytic actinomycetous isolates namely SG 1 , SG 2 , SG 3 and SS 1 were isolated from soil samples and provisionally identified as Streptomyces almquistii, S. caeruleus , S. hirsutus and S. endus, respectively. All the isolates showed heavy growth and liquefaction at 50˚C and pH 6.5 in Winstead's medium having 1.2% of CMC. The isolates were allowed to grow in Winstead's medium having Asparagine as a nitrogen source with different carbon sources for the maximum production of cellulases. The extracellular protein of the culture supernatant ranged from 1.14 μg /ml (SG 1 ) to 879.39 μg /ml (SG 3 ). The reducing sugar level of the culture supernatant ranged from 0.76 μg /ml (SG 2 ) to 558.33 μg /ml (SG 1 ). The highest CMC-ase activity (1431.81U/ml) was found with the crude enzyme of the strain SG 3 . The highest FP-ase activity (1087.11 U/ml) and Avicelase activity (1287.87U/ml) were found with the crude enzyme of SS 1 . To detrermine the optimum nitrogen sources, the isolates were allowed to grow in Winstead's medium having saw dust for SG 1 and SG 3 , dry leaf for SS 1 and SG 2 as a carbon source with different nitrogen sources for the maximum production of cellulases. The extracellular protein of the culture supernatant ranged from 35.50 μg /ml (SG 1 ) to 328.62 μg /ml (SS 1 ) and the reducing sugar level of the culture supernatant ranged from 3.79 μg /ml (SG 1 ) to 114.39 μg /ml (SG 3 ). However the highest CMC-ase activity (1353.78 U/ml), and FP-ase activity (215.90 U/ml) were found with the crude enzyme of the strain SG 2 and Avicelase activity (356.06U/ml) was found with the crude enzyme of the isolate SS 1 .

Production and Optimization of Cellulase Activity of Thermomonospora Viridis Isolated From Rice Straw

“…NEAE-D isolated from rice straw showed highest cellulase production at pH 6.5 (El-Naggar and Abdelwahed 2012) and similar report was made by other workers with four Streptomyces spp. isolated from different cellulosic substrates (Alam et al 2011).…”