Isolation, identification, and characterization of pathogenic Aeromonas hydrophila from critically endangered Acipenser baerii

Bakiyev, Serik; Smekenov, Izat; Zharkova, Irina; Kobegenova, Saidina; Sergaliyev, Nurlan; Absatirov, Gaisa; Bissenbaev, Amangeldy K.

doi:10.1016/j.aqrep.2022.101293

Cited by 4 publications

(2 citation statements)

References 55 publications

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“…This pathogen contributes to various diseases in aquaculture animals, including motile Aeromonas septicemia and red sore disease, which can lead to significant economic losses due to mass mortality [ 3 ]. Moreover, Aeromonas hydrophila is an emerging enteric and foodborne pathogen causing infectious diseases in humans, such as cholestasis, necrotizing fasciitis, acute gastroenteritis, ascites, and cloacal hemorrhage, thus posing a threat to the quality and safety of aquatic products and human health [ 4 , 5 , 6 , 7 , 8 ]. At present, antibiotic therapy is the primary treatment for Aeromonas hydrophila infections, and no effective cure exists.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Rapid LAMP Assay for Aeromonas hydrophila and Comparison with the Application of qPCR

et al. 2023

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The development of an exceptionally sensitive diagnostic technique for early identification of aquaculture diseases, specifically Aeromonas hydrophila, is essential for efficient management of disease outbreaks at aquaculture locations. In this research, a swift and sensitive diagnostic assay employing Loop-mediated isothermal amplification (LAMP) of Aeromonas hydrophila was devised and compared to the conventional qPCR method documented by Rong Wang. Validation of the diagnostic assay was carried out using actual samples obtained from aquaculture fish. The findings revealed that based on the rapid detection of crude bacterial genomic DNA, the fluorescent LAMP assay possessed a lower limit of detection (LOD) of 0.559 ng/μL (0.315–1.693, 95% CI), while the LOD for qPCR stood at 4.301 ng/μL (2.084–8.876, 95% CI). Both techniques demonstrated outstanding specificity, exhibiting no cross-reactivity with bacteria from the same or closely related genera. A total of 74 fish samples suspected to be infected with the fish disease were gathered, with 26 and 23 samples testing positive for Aeromonas hydrophila via LAMP and qPCR, respectively. The concordance analysis for LAMP and qPCR methods generated a Kappa value of 0.909 (0.778–1.000, 95% CI), signifying a high degree of diagnostic consensus. This study highlights that the LAMP assay eliminates the thermal cycle temperature change process of qPCR, uses lysate to crudely extract bacterial genomic DNA, and can complete the detection within 40 min, rendering it a practical and efficient alternative for monitoring disease outbreaks at aquaculture sites.

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Section: Introductionmentioning

confidence: 99%

Establishment of a Rapid LAMP Assay for Aeromonas hydrophila and Comparison with the Application of qPCR

et al. 2023

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“…Aquatic animals infected by MAS exhibit symptoms such as cloacal hemorrhage ( 10 ), ascites ( 11 ), gastroenteritic hemorrhage ( 12 ), and septicemia ulceration on the skin ( 13 ). Furthermore, the A. hydrophila strain will determine the appearance of the symptoms in the infected fish ( 14 ). Serine protease (ser), cytotoxins and aerolysin (aer) are the common virulence factors that contribute to A. hydrophila pathogenicity ( 15 ).…”

Section: Introductionmentioning

confidence: 99%

Role of phytobiotics in relieving the impacts of Aeromonas hydrophila infection on aquatic animals: A mini-review

Kari

Wee²,

Sukri³

et al. 2022

Front. Vet. Sci.

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Aeromonas hydrophila is a ubiquitous bacterium with various hosts that causes mass mortality in farm-raised fish species and significant economic losses. The current antibiotic treatment is ineffective in controlling this bacterium infection in aquaculture species. Therefore, an evaluation of potential phytobiotics is needed to find an alternative antimicrobial agent to reduce the over-reliance on antibiotics in aquaculture and safeguard public and environmental health. Furthermore, the rise in antibiotic resistance cases among pathogenic bacteria indicates an urgent need for new fish and shellfish health management solutions. In this context, phytobiotics applications in aquaculture can be defined as any medicinal plant-based antimicrobial agent used in fish and shellfish health management. This review will focus on the impacts of Motile Aeromonas Septicemia (MAS) due to A. hydrophila in aquaculture, the potential of phytobiotics in enhancing the tolerance of aquaculture species against MAS and the combination of phytobiotics with other antimicrobial and therapeutic agents against MAS.

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Dietary chitosan alleviates intestinal and liver injury of hybrid sturgeon (Acipenser baerii♀ × A. schrenckii♂) induced by Aeromonas hydrophila infection

Wang

et al. 2023

Animal Feed Science and Technology

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Isolation, identification, and characterization of pathogenic Aeromonas hydrophila from critically endangered Acipenser baerii

Cited by 4 publications

References 55 publications

Establishment of a Rapid LAMP Assay for Aeromonas hydrophila and Comparison with the Application of qPCR

Establishment of a Rapid LAMP Assay for Aeromonas hydrophila and Comparison with the Application of qPCR

Role of phytobiotics in relieving the impacts of Aeromonas hydrophila infection on aquatic animals: A mini-review

Dietary chitosan alleviates intestinal and liver injury of hybrid sturgeon (Acipenser baerii♀ × A. schrenckii♂) induced by Aeromonas hydrophila infection

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