Isolation, Identification and Antimicrobial Susceptibility Pattern of Tatumella ptyseos Strains Isolated From Powdered Infant Formula Milk Consumed in Neonatal Intensive Care Unit: First Report From Iran

Abstract

Background:Tatumella ptyseos is a rod-shaped, Gram-negative, facultative, and anaerobic bacteria categorized in the Enterobacteriaceae family. It is a rare food-borne opportunistic pathogen which causes neonatal sepsis, bacteremia, and urinary tract infections. T. ptyseos has been also cultured from various food sources around the world.Objectives:It is difficult to determine the source of the infection in the patients (especially newborns) due to low information about the epidemiology of T. ptyseos. The current study aimed to investigate the isolation, identification and antimicrobial susceptibility pattern of T. ptyseos strains from the consumed powdered infant formula milk (PIF) in hospital neonatal intensive care unit (NICU).Materials and Methods:A total of 125 powdered infant formula milk (PIF) samples were purchased from drug stores from June 2011 to March 2012. T. ptyseos was isolated according to food and drug administration (FDA) method. For final confirmation, biochemical testes embedded in API-20E system were used. Drug susceptibility test was performed using the disc diffusion method, according to clinical and laboratory standard institute (CLSI) recommendations.Results:Results of the study showed that, out of 125 samples, T. ptyseos was isolated from four (3/2%) PIF samples. All isolated strains (100%) were resistant to ampicillin, carbenicillin, cotrimoxazole and amoxicillin.Conclusions:The present study was the first report on the isolation and identification of T. ptyseos from PIF in Iran. T. ptyseos are frequently present in various kinds of foods; therefore, further investigation on these samples is required. It is necessary to track the T. ptyseos in a wide variety of foods and individuals especially in immunocompromised people such as human immunodeficiency virus (HIV)-positive patients to reveal the possible routes of transmission of this pathogen to humans. In addition, molecular studies are required to determine the genetic relationship between T. ptyseos strains isolated from different sources.

Molecular Detection of Diffusely Adherent Escherichia coli Strains Associated with Diarrhea in Shiraz, Iran

“…Our findings are similar to those of the studies from Iran, Peru, Vietnam, Brazil and Mexico (29)(30)(31)(32)(33)(34). It should be also noted that in many cases, antibiotic resistance is transmitted to humans, hospitalized patients and the hospital environment through other sources, including food plants, animals, fish, poultries, and other industries, in which antibiotics are used for different purposes and may lead to the emergence of resistant strains (35)(36)(37)(38). The isolation, identification and antimicrobial susceptibility of pathogens can be helpful in optimizing the use of antimicrobial agents.…”

Study of Cronobacter sakazakii Strains Isolated from Powdered Milk Infant Formula by Phenotypic and Molecular Methods in Iran

“…The reason is that they are usually fed with PIF, which is the most common route of transmission of the bacterium (22,29). Different foodborne disease-causing opportunistic bacteria are included in the Enterobacteriaceae family, and the point to be noted is that immunosuppressed persons are prime candidates for infections caused by these opportunistic pathogens (30)(31)(32)(33)(34).…”

Draft Genome Sequence of a Pantoea sp. Isolated from a Preterm Neonatal Blood Sepsis Patient

“…are Gram-negative, rare, opportunistic pathogens that can infect immune-compromised patients. They cause urinary infections and blood sepsis (1–3) and specifically Pantoea agglomerans has been linked to several outbreaks in neonatal units (1, 4, 5). Preterm neonates are a highly susceptible patient group for bacterial infections (6–8) and rapid detection of blood sepsis and identification of the causative agent are critical to enable proper treatment (9–11).…”