Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein

Abstract

A search for variant endogenous cat viruses led to a novel isolate. Although the major envelope glycoprotein of this virus was similar in size to that of an RD-114-like virus that was coisolated, it was unrelated to RD-114 or feline leukemia virus by immunological and biological criteria. This degree of dissimilarity suggests a different evolutionary progenitor from that for the RD-114 and feline leukemia virus viral envelopes. The novel virus did, however, code for gag gene polypeptides which are closely related to RD-114 virus. Neither the novel isolate nor the RD-114-like coisolate induced foci in S'Lcat cells which restrict focus induction by RD-114 virus. This suggests that the two viruses share a common genomic target of restriction which resides outside of the env region.

Isolation of an Infectious Endogenous Retrovirus in a Proportion of Live Attenuated Vaccines for Pets

“…ERVs are in general nonpathogenic for their host species, and RD-114 replication has been found to be restricted in some cat cell lines (4,5,12). However, cat cell lines fully susceptible to RD-114 replication have also been described (7). Thus, one would expect that exposure to RD-114 will be of little consequence to cats, given that RD-114 is an ERV of this species.…”

Identification of a retinoic acid-inducible endogenous retroviral transcript in the human teratocarcinoma-derived cell line PA-1

“…assay (12). RA-treated but not control dimethyl sulfoxidetreated cells were highly permissive for infection ( Table 1).…”

“…The cells were washed twice with 4 ml of medium, and then 5 ml of fresh medium with RA was added and the incubation was continued. When the cells were about to reach confluent growth, medium was collected and a PG-4 assay was performed as described previously (12). The cells were replated at 2 x i05 cells per 60-mm-diameter dish.…”

RevM10-expressing T cells derived in vivo from transduced human hematopoietic stem-progenitor cells inhibit human immunodeficiency virus replication

“…Amphotropic retrovirus vector supernatants were prepared as described previously (16), using the ProPak-A packaging cell line (36). All producer cells tested negative for the presence of replication-competent retroviruses by S ϩ L Ϫ assay on PG4 cells (18).…”