Isolation, fatty acid profiles and cryopreservation of marine thraustochytrids from mangrove habitats in Thailand

Unagul, Panida; Suetrong, Satinee; Preedanon, Sita; Klaysuban, Anupong; Gundool, Wunna; Suriyachadkun, Chanwit; Sakayaroj, Jariya

doi:10.1515/bot-2016-0111

Cited by 21 publications

(12 citation statements)

References 38 publications

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“…Microscopic observation revealed opaque white colonies of A. limacinum strain BUCHAXM 122 on GYP agar with dominant morphological characteristics similar to those of A. limacinum strain SR21 [20] and A. limacinum previously reported in Thailand [5,21]. A. limacinum differs from other Aurantiochytrium species in its characteristic feature of colony margins and amoeboid cells.…”

Section: Discussionsupporting

confidence: 53%

“…The content of other polyunsaturated fatty acids, such ARA, EPA and DPA in A. limacinum BUCHAXM 122 was lower than in Parietichtrium sarkarianum [21], Ulkenia [31] and Thraustochytrium [21,31]. Although PUFA amounts found in A. limacinum BUCHAXM 122 were less than in other microorganisms, an advantage remains for its future use: PUFA from thraustochytrids, especially Aurantiochytrium sp.…”

Section: Discussionmentioning

confidence: 94%

“…Rough colony margins were probably a result of creeping of amoeboid cells. Vegetative cell size of A. limacinum strain BUCHAXM 122 (5-10 µm) was smaller than A. limacinum SR21 (7-15 µm) [20] and A. limacinum (5-20 µm) [21].…”

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confidence: 80%

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Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Sirirak¹,

Suanjit²,

Powtongsook³

et al. 2020

ScienceAsia

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Aurantiochytrium limacinum BUCHAXM 122, a strain of thraustochytrid microorganism is potentially an alternative source of essential nutrients for commercial products. In this study, the strain was screened for its characterization and determination of polyunsaturated fatty acid (PUFA) production. Cells were isolated from fallen mangrove leaves, and grown in a glucose-yeast extract-peptone (GYP) medium. Their morphology, life cycle, biomass, fatty acid, extracellular enzymes and major elements were also investigated. Colonies occurred prominently on the fourth day of cultivation, appearing opaque white in color and containing large numbers of amoeboid cells. These thraustochytrids were observed to have a multi-stage life cycle, developing through zoospore, vegetative and zoosporangium stages with a life span of approximately 24 h. Zoospores developed into vegetative cells within 10-12 h and vegetative cells developed into zoosporangia, releasing zoospores, within approximately 10-15 h. Results showed that A. limacinum BUCHAXM 122 produced highest biomass at 108 h, with 23.85 ± 1.02 g/l dry weight. The isolates had high content of docosahexaenoic acid (DHA; 30.31 ± 5.88% of total fatty acids), indicating that they could serve as a fatty acid source for human consumption and aquaculture feeds. Major elemental analysis showed that cells contained 57% carbon, 29% oxygen, 9% hydrogen, 4% nitrogen and 1% sulfur. Extracellular degradative enzymes including protease, lipase, urease, α-glucosidase and phosphatase activities were also detected.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 94%

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Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Sirirak¹,

Suanjit²,

Powtongsook³

et al. 2020

ScienceAsia

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“…Labyrinthulomycetes are the most ubiquitous and widespread straminipilan protists in oceans with a total of 13 known genera and 30 described species so far [10]. They have been isolated, using the direct-plating and pollen-baiting methods, from various habitats (e.g., marine seawater, estuarine water, sediments, decaying mangrove leaves, and senescent macroalgae) [11,12,13,14,15,16]. Yet, the majority of the species described are isolated from only a limited number of coastal regions, for example, Japan [17], Australia [12,18], Canada [19], India [20,21,22], Chile [11], China [23], Malaysia [24,25], and Thailand [26].…”

Section: Introductionmentioning

confidence: 99%

Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China

Wang

Xie

et al. 2019

Marine Drugs

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Labyrinthulomycete protists have gained significant attention in the recent past for their biotechnological importance. Yet, their lipid profiles are poorly described because only a few large-scale isolation attempts have been made so far. Here, we isolated more than 200 strains from mangrove habitats of China and characterized the molecular phylogeny and lipid accumulation potential of 71 strains. These strains were the closest relatives of six genera namely Aurantiochytrium, Botryochytrium, Parietichytrium, Schizochytrium, Thraustochytrium, and Labyrinthula. Docosahexaenoic acid (DHA) production of the top 15 strains ranged from 0.23 g/L to 1.14 g/L. Two labyrinthulid strains, GXBH-107 and GXBH-215, exhibited unprecedented high DHA production potential with content >10% of biomass. Among all strains, ZJWZ-7, identified as an Aurantiochytrium strain, exhibited the highest DHA production. Further optimization of culture conditions for strain ZJWZ-7 showed improved lipid production (1.66 g/L DHA and 1.68 g/L saturated fatty acids (SFAs)) with glycerol-malic-acid, peptone-yeast-extract, initial pH 7, 28 °C, and rotation rate 150 rpm. Besides, nitrogen source, initial pH, temperature, and rotation rate had significant effects on the cell biomass, DHA, and SFAs production. This study provides the identification and characterization of nearly six dozen thraustochytrids and labyrinthulids with high potential for lipid accumulation.

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“…Dried cells of microorganisms were extracted twice by maceration in hexane overnight at room temperature. Crude fat extract was individually transesterified with 4% sulphuric acid in methanol 64 . A fat extract was dissolved in methanol containing 4% of sulphuric acid, and the mixture was heated at 90 °C for 1 h. Nonadecanoic acid (C19:0) was used as an internal standard.…”

Section: Methods Cultivation Of Microorganismsmentioning

confidence: 99%

An anticonvulsive drug, valproic acid (valproate), has effects on the biosynthesis of fatty acids and polyketides in microorganisms

Poolchanuan

Unagul

Thongnest

et al. 2020

Sci Rep

Self Cite

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Valproic acid or valproate (VpA) is an anticonvulsive drug used for treatments of epilepsy, bipolar disorder, and migraine headaches. VpA is also an epigenetic modulator, inhibiting histone deacetylase, and it has been subjected to clinical study for cancer treatment. During the investigation of VpA on a metabolite profile in a fungus, we found that VPA has significant effects on the production of some fatty acids. Further exploration of VPA on fatty acid profiles of microorganisms, fungi, yeast, and bacteria, as well as representative gut microbiome, revealed that VpA could enhance or reduce the production of some fatty acids. VpA was found to induce the production of trans-9-elaidic acid, a fatty acid that was previously reported to have cellular effects in human macrophages. VPA could also inhibit the production of some polyketides produced by a model fungus. the present work suggests that the induction or inhibition of fatty acid biosynthesis by VPA (100 µM) in gut microbiome could give effects to patients treated with VPA because high doses of VPA oral administration (up to 600 mg to 900 mg) are used by patients; the concentration of VPA in the human gut may reach a concentration of 100 µM, which may give effects to gut microorganisms. Valproic acid (VPA) or valproate, an anticonvulsive drug, has been used as drug for the treatment of epilepsy, bipolar disorder, and for the prevention of migraine headaches. VPA has side effects, for example, hepatic steatogenesis in rats 1 , and negative influence on hepatic carbohydrate and lipid metabolism 2. There were a number of fatal cases of hyperammonemia for patients treated with VPA 3. In mitochondria, VPA undergoes fatty acid β-oxidation pathway, causing toxicity due to interference with mitochondrial β-oxidation, and many serious inborn errors of metabolism are caused by VPA treatment 4. VPA interferes with carnitine palmitoyl-transferase I, a key enzyme in mitochondrial fatty acid β-oxidation, and thus inducing hepatotoxicity and weight gain for patients under VPA therapy 5. VPA could inhibit N-acetyl glutamate synthetase, and thus inhibiting urea synthesis 6,7. VPA was found to induce abnormal autism-like behaviors in mice 8. Recently, a number of research groups paid attention on the risk of VPA on autism spectrum disorders 9-14. The discovery that VPA is an inhibitor of histone deacetylase, a promising anticancer drug target, has stimulated the scientific community worldwide to investigate the detailed mechanisms of VPA in various aspects 15,16. Recently, drug repurposing of VPA has been intensively explored for the treatment of various diseases, for example, the treatment of breast cancer 17 , colon cancer associated with diabetes mellitus 18 , diffuse intrinsic pontine glioma 19 , high-fat diet-induced hypertension 20 ,

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Isolation, fatty acid profiles and cryopreservation of marine thraustochytrids from mangrove habitats in Thailand

Cited by 21 publications

References 38 publications

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Culturable Diversity and Lipid Production Profile of Labyrinthulomycete Protists Isolated from Coastal Mangrove Habitats of China

An anticonvulsive drug, valproic acid (valproate), has effects on the biosynthesis of fatty acids and polyketides in microorganisms

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