Isolation, expression analysis and chromosomal location of P5CR gene in common wheat (Triticum aestivum L.)

Ma, Lin; Zhou, Erfeng; Gao, Lifeng; Mao, Xinguo; Zhou, Rong; Jia, Jizeng

doi:10.1016/j.sajb.2008.05.003

Cited by 41 publications

(17 citation statements)

References 41 publications

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“…). The transcript levels of P5CR increased with osmotic stress in prairie Junegrass and Arabidopsis . De Ronde et al .…”

Section: Discussionmentioning

confidence: 98%

“…The enzyme P5CS is rate‐limiting in proline synthesis. In many plant species, P5CS is encoded by two genes that have different expression patterns, and the enzymes have nonredundant roles of decreasing stress damage to the cell during development . Drought‐induced upregulation of P5CS1 and P5CS2 was detected in many plants including Kosteletzkya virginica (L.) C. Presl ex A.…”

Section: Discussionmentioning

confidence: 99%

“…3). The transcript levels of P5CR increased with osmotic stress in prairie Junegrass [8] and Arabidopsis [55]. De Ronde et al [58] overexpressed Arabidopsis P5CR in soybean, and the transgenic plant showed improved drought tolerance, indicating that expression of antisense P5CR leads to stress sensitivity.…”

Section: Effects Of Water Stress On Proline Metabolic Genesmentioning

confidence: 99%

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Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.

Soni

Abdin

2017

FEBS Open Bio

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Water stress is one of the most critical abiotic stresses that restricts growth, development, and alters physiological and biochemical mechanisms of plant. The effects of long‐term water shortage‐induced oxidative stress on morphophysiological parameters, proline metabolic genes, and artemisinin content were studied in Artemisia annua L. under greenhouse conditions. Plant growth, biomass accumulation, relative water content, and chlorophyll content were reduced under drought. Leaf water potential ranged from −0.3248 MPa to −1.22 MPa in stress conditions. Increased levels of proline accumulation, protein concentration, and lipid peroxidation were detected in water‐stressed plants. Stage‐dependent increases in activity of antioxidants including superoxide dismutase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase were observed. The expression of proline biosynthetic genes including pyrroline‐5‐carboxylase synthase1, 1‐pyrroline‐5‐carboxylase synthase2, and 1‐pyrroline‐5‐carboxylase reductase was induced, while the ornithine aminotransferase transcript showed a variable response and the expression of proline catabolic genes including proline dehydrogenase1, proline dehydrogenase1, and proline 5‐carboxylate dehydrogenase was reduced by water stress. Our results indicate that the glutamine pathway is predominant under drought stress in A. annua and a reduction of catabolic gene expression is adopted as a defense strategy in adverse conditions. Higher expression of biosynthetic genes and lower expression of catabolic genes at the preflowering stage confirmed the important role of proline in flower development. Artemisinin content decreased owing to water stress, but the slightly higher amounts were detected in leaves of severely stressed plants compared with moderately stressed plants. The artemisinin content of A. annua might be regulated by controlling irrigation regimes.

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“…). The transcript levels of P5CR increased with osmotic stress in prairie Junegrass and Arabidopsis . De Ronde et al .…”

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Effects Of Water Stress On Proline Metabolic Genesmentioning

confidence: 99%

See 1 more Smart Citation

Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.

Soni

Abdin

2017

FEBS Open Bio

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“…Though, several expression studies related to P5CS gene have been reported, a few are related to P5CR and GOGAT. Ma et al (2008) reported an upregulation of TaP5CR gene in wheat under salt, ABA, PEG and heat stress.…”

Section: Proline Accumulation and Gene Expression Analysismentioning

confidence: 98%

Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Rana

Ram

Nehra

et al. 2016

Cereal Research Communications

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In the present investigation, expression of genes related to Na + exclusion such as salt overly sensitive (TaSOS1) and Na + /H + antiporter (TaNHX1) and proline accumulation such as pyrroline-5-carboxylate reductase (P5CR) and glutamate synthase (GOGAT) was studied in seedlings of Kharchia 65 (Kh 65, salt tolerant) and HD 2009 (sensitive) under salt stress (ECe, 12 dSm -1 ) and controlled conditions. As compared to HD 2009, Kh 65 showed significantly lower accumulation of Na + (p < 0.01) and higher accumulation of proline (p < 0.05) in leaf blade under salt stress. The relative expression of TaSOS1 increased significantly (p < 0.001) in roots of Kh 65 (4.31-fold) while it decreased in HD 2009. There was significantly higher (p < 0.01) relative expression of TaNHX1 (27.57-fold) in leaf and 3.07-fold in roots of Kh 65 as compared to 3.65-and 0.87-fold increase in leaf and roots of HD 2009, respectively, under salt stress. There was significantly (p < 0.05) higher accumulation of proline as compared to HD 2009 in leaf tissues. There was significantly higher (p < 0.01) expression of P5CR (5.23-fold in leaf and 8.77-fold in the root) and glutamate synthase (6.0-fold in roots) in Kh 65 as compared to HD 2009. The study demonstrated that upregulation of genes for Na + exclusion in root and compartmentation in leaf and increased proline concentration are associated with tolerance to salinity stress in wheat. The information will be useful for improving wheat genotypes for salt tolerance.

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“…In the present case, E. coli cells transformed with P5CR, showed ∼10 % increment in growth. Increased P5C production against salinity and proline accumulation was attributed to enhanced P5CR activity (Ma et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance

Singh

Tiwari

Singh

et al. 2013

Physiol Mol Biol Plants

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Drought is the most important abiotic stress, challenging sustainable agriculture globally. For desiccation being the multigenic trait, a combination of identified genes from the appropriate organism may render crop tolerant to the water stress. Among the compatible solutes, proline plays multifaceted role in counteracting such stress. The genes encoding proline biosynthesizing enzymes, glutamate 5-kinase (G5K), and pyrroline-5-carboxylate reductase (P5CR) from the low-desiccation-tolerant cyanobacterium Anabaena sp. PCC 7120, were cloned and overexpressed in Escherichia coli BL21(DE3) individually. The recombinant E. coli cells harboring G5K, failed to exhibit enhanced desiccation tolerance relative to those with P5CR that showed increased growth/survival over the wild type. This may be ascribed to the overexpression of the reductase gene. Multiple sequence alignment showed P5CR to be conserved in all the organisms. We hypothesize that P5CR gene from high-desiccation-tolerant cyanobacteria may be adopted as the candidate for making transgenic N 2 -fixing cyanobacterium for paddy fields and/or crop development in future.

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Isolation, expression analysis and chromosomal location of P5CR gene in common wheat (Triticum aestivum L.)

Cited by 41 publications

References 41 publications

Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.

Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.

Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance

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Isolation, expression analysis and chromosomal location of P5CR gene in common wheat (Triticum aestivum L.)

Cited by 41 publications

References 41 publications

Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.

Water deficit‐induced oxidative stress affects artemisinin content and expression of proline metabolic genes in Artemisia annua L.

Expression of genes related to Na+exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance

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Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress