Isolation, evaluation and characterization of Bacillus subtilis from cotton rhizospheric soil with biocontrol activity against Fusarium oxysporum

Abstract

Present investigation is based on the isolation of Bacillus subtilis from cotton rhizosphere and their evaluation as biocontrol agent against Fusarium oxysporum. The production of extracellular hydrolytic enzyme was studied for determining the antagonism. 43% of 21 isolates were identified under the B. subtilis group on the basis of biochemical characterization. 38% isolates showed competitive activity against Fusarium oxysporum and exhibit more than 50% mycelial inhibition in dual culture bioassay. The pot assay of cotton by seed treatment and soil amendment technique under green house condition showed the competent activity of the isolates in preventing the wilting of cotton seedlings due to F. oxysporum infection. SVI values of 30 day old seedlings indicated that the soil inoculation with B. subtilis BP-2 and seed treatment with B. subtilis BP-9 significantly promoted the growth of cotton seedlings. RAPD profiling revealed the diversity in the Bacillus subtilis group, ranging from 10 to 32%. The discriminative pattern among the isolates belonging to the same species was validated by 16S rDNA partial sequencing which identified them into four different strains of B. subtilis.

Plant growth-promoting endophytic bacteria versus pathogenic infections: an example ofBacillus amyloliquefaciensRWL-1 andFusarium oxysporumf. sp.lycopersiciin tomato

“…Members of Bacillus and Pseudomonas are most reported for plant growth promoting and stress mediating seed endophytes (Chaves-López et al, 2015; Sundaramoorthy & Balabaskar, 2013; Choudhary & Johri, 2009). The species belonging to Bacillus are known as plant growth promoters that can increase crop growth and productivity (Quan et al, 2006; Gajbhiye et al, 2010). The bio-control potential of Bacillus licheniformis, Bacillus subtilis, Bacillus cereus, Bacillus pumilus , and Bacillus amyloliquefaciens has been documented in numerous reports (Han et al, 2016; Pane & Zaccardelli, 2015).…”

“…These microbial resources can influence the fitness and survival of plants, either beneficially or antagonistically (Bardgett & van der Putten, 2014). Soil-borne plant pathogens are hazardous to the plant growth and productivity (Gajbhiye et al, 2010). Most soil-borne pathogens survive in soil for long periods of time where they remain dormant until they find a suitable host (Vurro & Gressel, 2006).…”

Effectiveness of Resistant Germplasm and Biological Control Agents as a Sustainable Management for Fusarium Wilt Disease on Chickpea

“…harzianum (Whipps et al, 2001) and B. subtilis (Wulff et al, 2002;Gajbhiye et al, 2010;Chen et al, 2010) as effective bio-control agents against F. oxysporum f. sp. ciceris.…”

Characterization of plant growth-promoting traits of Penicillium species against the effects of high soil salinity and root disease

“…Harmful fungi in soil can cause plant diseases, and among these, Fusarium spp. infect the roots of crop plants (Gajbhiye et al 2010;Hariprasad et al 2011). Fusarium spp.…”