Isolation, endocrine regulation and transcript distribution of a putative primary JH-responsive gene from the pine engraver, Ips pini (Coleoptera: Scolytidae)

Bearfield, Jeremy C.; Box, C.D.; Keeling, Christopher I.; Young, Sharon M.; Blomquist, Gary J.; Tittiger, Claus

doi:10.1016/j.ibmb.2007.11.009

Cited by 4 publications

(3 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strong induction of gene expression of members of this gene family, together with well-known detoxification genes such as cytochrome P450 or glutathione-S-transferase, by xenobiotics such as phenobarbital and piperonyl butoxide has also been found in D. melanogaster (King-Jones et al, 2006;Willoughby et al, 2007). One of the early genes induced by juvenile hormone in the midgut of larval pine engraver beetle I. pini is a member of this gene family (Bearfield et al, 2008). In Caenorhabditis elegans, the transcriptional coregulator MDT-15 regulates genes implicated in metabolic responses to ingested food including specific detoxicative genes and a member of the DUF227 family.…”

Section: Discussionmentioning

confidence: 99%

Chromatographic and electrophoretic resolution of proteins and protein complexes from the larval midgut microvilli of Manduca sexta

Pauchet

Muck

Svatoš

et al. 2009

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Chromatographic and electrophoretic resolution of proteins and protein complexes from the larval midgut microvilli of Manduca sexta

Pauchet

Muck

Svatoš

et al. 2009

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

“…JhI-26 is an EcKL in Drosophila melanogaster (Diptera) that responds to juvenile hormone (JH) signalling (Dubrovsky et al 2000) and may play a role in Wolbachia-mediated cytoplasmic incompatibility (Liu et al 2014). Ipi10G08 (Genbank: AY875646.1) is a male-biased EcKL in the pine engraver beetle Ips pini (Coleoptera) that is also strongly inducible by JH (Bearfield et al 2008). Lastly, CHKov1 and CHKov2 are EcKLs in D. melanogaster implicated in resistance to sigma virus infection through an unknown molecular mechanism (Magwire et al 2011(Magwire et al , 2012Duxbury et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Phylogenomics of the ecdysteroid kinase-like (EcKL) gene family in insects highlights roles in both steroid hormone metabolism and detoxification

Scanlan

Robin

2023

Preprint

View full text Add to dashboard Cite

The evolutionary dynamics of large gene families can offer important insights into the functions of their individual members. While the ecdysteroid kinase-like (EcKL) gene family has previously been linked to the metabolism of both steroid moulting hormones and xenobiotic toxins, the functions of nearly all EcKL genes are unknown and there is little information on their evolution across all insects. Here, we perform comprehensive phylogenetic analyses on a manually annotated set of EcKL genes from 140 insect genomes, revealing the gene family is comprised of at least 13 subfamilies that differ in retention and stability. Our results show the only two genes known to encode ecdysteroid kinases belong to different subfamilies and therefore ecdysteroid metabolism functions must be spread throughout the EcKL family. We also provide comparative phylogenomic evidence that EcKLs are involved in detoxification across insects, with positive associations between family size and dietary chemical complexity, and also find similar evidence for the cytochrome P450 and glutathione S-transferase gene families. Unexpectedly, we find that the size of the clade containing a known ecdysteroid kinase is positively associated with host plant taxonomic diversity in Lepidoptera, possibly suggesting multiple functional shifts between hormone and xenobiotic metabolism. This work provides a robust framework for future functional studies into the EcKL gene family and opens promising new avenues for exploring the genomic basis of dietary adaptation in insects, including the classically studied co-evolution of butterflies with their host plants.

show abstract

“…JhI-26 is an EcKL in Drosophila melanogaster (Diptera) that responds to juvenile hormone (JH) signaling ( Dubrovsky et al 2000 ) and may play a role in Wolbachia -mediated cytoplasmic incompatibility ( Liu et al 2014 ). Ipi10G08 (Genbank: AY875646.1 ) is a male-biased EcKL in the pine engraver beetle Ips pini (Coleoptera) that is also strongly inducible by JH ( Bearfield et al 2008 ). Lastly, CHKov1 and CHKov2 are EcKLs in D. melanogaster implicated in resistance to sigma virus infection through an unknown molecular mechanism ( Magwire et al 2011 , 2012 ; Duxbury et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Phylogenomics of the Ecdysteroid Kinase-like (EcKL) Gene Family in Insects Highlights Roles in Both Steroid Hormone Metabolism and Detoxification

Scanlan,

Robin

2024

Genome Biology and Evolution

View full text Add to dashboard Cite

The evolutionary dynamics of large gene families can offer important insights into the functions of their individual members. While the ecdysteroid kinase-like (EcKL) gene family has previously been linked to the metabolism of both steroid moulting hormones and xenobiotic toxins, the functions of nearly all EcKL genes are unknown and there is little information on their evolution across all insects. Here, we perform comprehensive phylogenetic analyses on a manually annotated set of EcKL genes from 140 insect genomes, revealing the gene family is comprised of at least 13 subfamilies that differ in retention and stability. Our results show the only two genes known to encode ecdysteroid kinases belong to different subfamilies and therefore ecdysteroid metabolism functions must be spread throughout the EcKL family. We provide comparative phylogenomic evidence that EcKLs are involved in detoxification across insects, with positive associations between family size and dietary chemical complexity, and we also find similar evidence for the cytochrome P450 and glutathione S-transferase gene families. Unexpectedly, we find that the size of the clade containing a known ecdysteroid kinase is positively associated with host plant taxonomic diversity in Lepidoptera, possibly suggesting multiple functional shifts between hormone and xenobiotic metabolism. Our evolutionary analyses provide hypotheses of function and a robust framework for future experimental studies of the EcKL gene family. They also open promising new avenues for exploring the genomic basis of dietary adaptation in insects, including the classically studied co-evolution of butterflies with their host plants.

show abstract

Isolation, endocrine regulation and transcript distribution of a putative primary JH-responsive gene from the pine engraver, Ips pini (Coleoptera: Scolytidae)

Cited by 4 publications

References 67 publications

Chromatographic and electrophoretic resolution of proteins and protein complexes from the larval midgut microvilli of Manduca sexta

Chromatographic and electrophoretic resolution of proteins and protein complexes from the larval midgut microvilli of Manduca sexta

Phylogenomics of the ecdysteroid kinase-like (EcKL) gene family in insects highlights roles in both steroid hormone metabolism and detoxification

Phylogenomics of the Ecdysteroid Kinase-like (EcKL) Gene Family in Insects Highlights Roles in Both Steroid Hormone Metabolism and Detoxification

Contact Info

Product

Resources

About