Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene.

Atcheson, Catherine L.; DiDomenico, Beth; Frackman, Susan; Esposito, Rochelle Easton; Elder, Robert E.

doi:10.1073/pnas.84.22.8035

Cited by 86 publications

(61 citation statements)

References 27 publications

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“…After the completion of the two meiotic divisions, spore walls are laid down around the haploid nuclei, generating an ascus containing a tetrad of haploid spores that are more resistant to various lytic enzymes, to some organic solvents, and to high temperature than are vegetative cells (Dawes and Hardie 1974;Put and De Jong 1981). The series of genetic and morphological events that occurs as cells progress through the sporulation pathway has been shown to be accompanied by the sequential activation of temporally distinct classes of sporulation-specific genes (for review, see Magee 1987;Clancy et al 1983;Kurtz and Lindquist 1984;Percival-Smith and Segall 1984;Weir-Thompson and Dawes 1984;Holaway et al 1985;Yamashita and Fukui 1985;Gottlin-Ninfa and Kaback 1986;Atcheson et al 1987;Wang et al 1987;Law and Segall 1988;Thompson and Roeder 1989). Here, we describe the identification of two sporulation-specific genes that are required for spore wall maturation.…”

Section: Rme1 Ime1 and Ime2mentioning

confidence: 99%

Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae.

Briza¹,

Breitenbach²,

Ellinger³

et al. 1990

Genes Dev.

198

233

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During sporulation of Saccharomyces cerevisiae, the four haploid nuclei generated by meiosis are encapsulated within multilayered spore walls. Taking advantage of the natural fluorescence imparted to yeast spores by the presence of a dityrosine-containing macromolecule in the spore wall, we identified and cloned two genes, termed DIT1 and DIT2, which are required for spore wall maturation. Mutation of these genes has no effect on the efficiency of spore formation or spore viability. The mutant spores, however, fail to accumulate the spore wall-specific dityrosine and lack the outermost layer of the spore wall. The absence of this cross-linked surface layer reduces the resistance of the spores to lyric enzymes, to ether, and to elevated temperature. Expression of the DIT and DIT2 genes is restricted to sporulating cells, with the DIT1 transcripts accumulating at the time of prospore enclosure and just prior to the time of dityrosine biosynthesis. Both genes act in a sporeautonomous manner implying that at least some of the activities responsible for forming the outermost layer of the spore wall reside within the developing spore rather than in the surrounding ascal cytoplasm. As the DIT2 gene product has significant homology with cytochrome P-450s, DIT2 may be responsible for catalyzing the oxidation of tyrosine residues in the formation of dityrosine.

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Section: Rme1 Ime1 and Ime2mentioning

confidence: 99%

Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae.

Briza¹,

Breitenbach²,

Ellinger³

et al. 1990

Genes Dev.

198

233

View full text Add to dashboard Cite

show abstract

“…HOPl, which is is required for pairing of homologous chromosomes (Hollingsworth and Byers 1989;Hollingsworth et al 1990), is expressed coincidentally with a large number of other meiotic genes such as spoil (Atcheson et al 1987), REDl (Thompson and Roeder 1989), and REC114 (Pittman et al 1993), which encode products required for successful comple tion of meiosis (for review, see Mitchell 1994). DITl, which is expressed just prior to spore formation, is re quired for deposition of the outermost layer of the spore wall (Briza et al 1990b).…”

Section: Spsl Is Expressed As Meiosis Is Nearing Completionmentioning

confidence: 99%

Mutation of the SPS1-encoded protein kinase of Saccharomyces cerevisiae leads to defects in transcription and morphology during spore formation.

Friesen¹,

Lunz²,

Doyle³

et al. 1994

Genes Dev.

116

132

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During spoiulation of Saccharomyces cerevisiae, meiosis is followed by encapsulation of haploid nuclei within multilayered spore walls. Completion of the late events of the sporulation program requires the SPSl gene. This developmentally regulated gene, which is expressed as cells are nearing the end of meiosis, encodes a protein with homology to serine/threonine protein kinases. The catalytic domain of Spsl is 44% identical to the kinase domain of yeast Ste20, a protein involved in the pheromone-induced signal transduction pathway. Cells of a MATa./MATa. spsl/spsl strain arrest after meiosis and fail to activate genes that are normally expressed at a late time of sporulation. The mutant cells do not form refractile spores as assessed by phase-contrast microscopy and do not display the natural fluorescence and ether resistance that is characteristic of mature spores. Examination by electron microscopy reveals, however, that prospore-like compartments form in some of the mutant cells. These immature spores lack the cross-linked surface layer that surrounds wild-type spores and are more variable in size and number than are the spores of wild-type cells. Despite their inability to complete spore formation, spsl-arrested cells are able to resume mitotic growth on transfer to rich medium, generating haploid progeny. Our results suggest that the developmentally regulated Spsl kinase is required for normal progression of transcriptional, biochemical, and morphological events during the later portion of the sporulation program.

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“…The early meiotic recombination gene, SPO11, has a potential URS1 sequence in the coding region at position bp +163 (Atcheson et al, 1987;Mitchell, 1994). This was a surprising result since most S. cerevisiae genes are not known to have downstream regulatory sequences for transcription (e.g.…”

Section: Conservation Of Regulatory Sites In Rec102mentioning

confidence: 99%

Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

Jiao

Nau

Cool

et al. 2001

Yeast

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REC102 is a meiosis-specific early exchange gene absolutely required for meiotic recombination in Saccharomyces cerevisiae. Sequence analysis of REC102 indicates that there are multiple potential regulatory elements in its promoter region, and a possible regulatory element in the coding region. This suggests that the regulation of REC102 may be complex and may include elements not yet reported in other meiotic genes. To identify potential cis-regulatory elements, phylogenetic footprinting analysis was used. REC102 homologues were cloned from other two Saccharomyces spp. and sequence comparison among the three species defined evolutionarily conserved elements. Deletion analysis demonstrated that the early meiotic gene regulatory element URS1 was necessary but not sufficient for proper regulation of REC102. Upstream elements, including the binding sites for Gcr1p, Yap1p, Rap1p and several novel conserved sequences, are also required for the normal regulation of REC102 as well as a Rap1p binding site located in the coding region. The data in this paper support the use of phylogenetic comparisions as a method for determining important sequences in complex promoters.

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Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene.

Cited by 86 publications

References 27 publications

Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae.

Isolation of two developmentally regulated genes involved in spore wall maturation in Saccharomyces cerevisiae.

Mutation of the SPS1-encoded protein kinase of Saccharomyces cerevisiae leads to defects in transcription and morphology during spore formation.

Phylogenetic footprinting reveals multiple regulatory elements involved in control of the meiotic recombination gene, REC102

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