Isolation, development and validation of HPTLC method for the estimation of β-carotene from Gymnosporia senegalensis (Lam.) Loes.

Jain, Divya; Meena, Mukesh; Singh, D.; Janmeda, Pracheta

doi:10.1016/j.plaphy.2023.107843

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…According to the results of this study, G. senegalensis can serve as a good source of antioxidants for pharmaceutical preparations and applications that enable the cellular system to cope with oxidative stress and alleviate the toxic effects of the administrative compound. With the knowledge of different radical scavenging properties, it becomes easy to establish a relationship between the endogenous and exogenous antioxidant systems, which is important for the naturally derived herbal drugs’ functioning and bioavailability in the body [ 69 , 70 , 71 , 72 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Quantitative Phytochemical Content and Antioxidant Activity of Leaf, Stem, and Bark of Gymnosporia senegalensis (Lam.) Loes.

Jain,

Meena,

Janmeda

et al. 2024

Plants

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To the best of our knowledge, there was no prior report providing valuable preliminary data through a demonstration of the quantitative phytochemical and antioxidant activity of Gymnosporia senegalensis. The total contents of phenols, flavonoid, flavanol, tannin, and saponin were evaluated from different fractions extracted from the leaf, stem, and bark of G. senegalensis by using standards such as gallic acid, quercetin, rutin, tannic acid, and saponin quillaja. The antioxidant potential was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide scavenging (H2O2), superoxide anion radical scavenging, metal chelating ferrous ion, ferric reducing antioxidant power (FRAP), and total antioxidant capacity (TAC). Data were subjected to half-inhibitory concentration (IC50) and one-way analysis of variance (ANOVA) at p < 0.05 as a significant value. The total phenol content was found to be highest in the chloroform extract of stem at 97.7 ± 0.02 mg GAE/g. The total flavonoid and flavonol contents in the aqueous extract were 97.1 ± 0.03 mg QE/g and 96.7 ± 0.07 mg RE/g, respectively. The total tannin content in the ethyl acetate extract of leaf was 97.5 ± 0.01 mg TAE/g, and the total saponin content in the methanol extract of stem was 79.1 ± 0.06 mg SQE/g. The antioxidant analysis indicated that IC50 and percentage (%) inhibition were dose-dependent and showed the highest antioxidant activity (40.9 ± 0.9 µg/mL) in methanol extract of leaf for DPPH, (88.8 ± 1.12 µg/mL) in the chloroform extract of stem for H2O2, (43.9 ± 0.15 µg/mL) in the aqueous extract of bark for superoxide anion radical scavenging activity, (26.9 ± 0.11 µg/mL) in the chloroform extract of leaf for the metal chelating ferrous ion activity, (7.55 ± 0.10 mg/mL) in the benzene extract of leaf for FRAP, and (2.97 ± 0.01 mg/mL) in the methanol extract of bark for TAC. These results show that G. senegalensis has great potential in antioxidant activities. The isolation and characterization of specific bioactive compounds and the in vivo applicability of such activity await further extensive studies for drug discovery and development.

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Section: Discussionmentioning

confidence: 99%

Analysis of Quantitative Phytochemical Content and Antioxidant Activity of Leaf, Stem, and Bark of Gymnosporia senegalensis (Lam.) Loes.

Jain,

Meena,

Janmeda

et al. 2024

Plants

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Development of a simple and reproducible HPTLC method on protective effect induced by bark of Acacia nilotica on poisoning caused by use of nicotine containing tobacco products

Jha,

Parihar,

Sahu

et al. 2024

Journal of Chromatography B

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Structural characterisation of bioactive compounds of Gymnosporia senegalensis (Lam.) Loes. using advanced analytical technique like FT-IR, GC-MS and ¹ H-NMR spectroscopy

Jain,

Meena,

Singh

et al. 2023

Natural Product Research

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Isolation, development and validation of HPTLC method for the estimation of β-carotene from Gymnosporia senegalensis (Lam.) Loes.

Cited by 3 publications

References 30 publications

Analysis of Quantitative Phytochemical Content and Antioxidant Activity of Leaf, Stem, and Bark of Gymnosporia senegalensis (Lam.) Loes.

Analysis of Quantitative Phytochemical Content and Antioxidant Activity of Leaf, Stem, and Bark of Gymnosporia senegalensis (Lam.) Loes.

Development of a simple and reproducible HPTLC method on protective effect induced by bark of Acacia nilotica on poisoning caused by use of nicotine containing tobacco products

Structural characterisation of bioactive compounds of Gymnosporia senegalensis (Lam.) Loes. using advanced analytical technique like FT-IR, GC-MS and ¹ H-NMR spectroscopy

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Isolation, development and validation of HPTLC method for the estimation of β-carotene from Gymnosporia senegalensis (Lam.) Loes.

Cited by 3 publications

References 30 publications

Analysis of Quantitative Phytochemical Content and Antioxidant Activity of Leaf, Stem, and Bark of Gymnosporia senegalensis (Lam.) Loes.

Analysis of Quantitative Phytochemical Content and Antioxidant Activity of Leaf, Stem, and Bark of Gymnosporia senegalensis (Lam.) Loes.

Development of a simple and reproducible HPTLC method on protective effect induced by bark of Acacia nilotica on poisoning caused by use of nicotine containing tobacco products

Structural characterisation of bioactive compounds of Gymnosporia senegalensis (Lam.) Loes. using advanced analytical technique like FT-IR, GC-MS and 1 H-NMR spectroscopy

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Structural characterisation of bioactive compounds of Gymnosporia senegalensis (Lam.) Loes. using advanced analytical technique like FT-IR, GC-MS and ¹ H-NMR spectroscopy