Isolation, density purification, and in vitro culture maintenance of functional caprine islets of Langerhans as an alternative islet source for diabetes study

Abstract

The successful yield, viability and functionality of islets isolated from the optimized protocol provide promising potential as an alternative source of islets for diabetes and transplantation researches.

Sero-epidemiology of Contagious Ecthyma Based on Detection of IgG Antibody in Selected Sheep and Goats Farms in Malaysia

“…Detail studies on adaptation of orf virus in tissue culture and embryonating eggs would pave the way for further studies to elucidate orf virus biology, mechanisms and preparation of the virus for vaccines to control and eradicate the disease Sadiq et al, 2017;Bala et al, 2018). It would be rather intriguing to determine the capability of orf virus to grow in cell lines of the original host perhaps to affect cell growth in vitroas well (Vakhshiteh et al, 2013;Hani et al, 2010). Besides parapoxviruses, herpesvirus family also portraying similar characteristics to grow in cell lines PCR and viral isolation were employed as confirmatory diagnosis for diseases.…”

Analysis of selected glutathione S-transferase gene polymorphisms in Malaysian type 2 diabetes mellitus patients with and without cardiovascular disease

“…The pancreas plays an important role in blood glucose control and is affected by carcinogens present in cigarette smoke, making it susceptible to the development of cancer. Moreover, due to the lack of available human donors, it is increasingly necessary to investigate alternative sources, such as cultured islet cells, for future pancreatic transplantation (Hani et al, 2010). GSTM1 polymorphisms have been extensively studied in gastric (La Torre et al, 2005), colon (Ye and Parry, 2003), head and neck (Hashibe et al, 2003;Ye et al, 2004), prostate (Ntais et al, 2005), lung (Benhamou et al, 2002), bladder (Johns and Houlston, 2000), and breast cancer (Sull et al, 2004;Vogl et al, 2004).…”

Xenotransplantation literature update: November–December, 2010

“…As an alternative to pig pancreatic islets, Hani et al [7] investigated the potential of caprine (goat) as a source for islet transplantation. Caprine islets were obtained by a collagenase-based digestion and demonstrated 90% viability in a culture system.…”