Isolation, culture and immortalisation of hepatic oval cells from adult mice fed a choline-deficient, ethionine-supplemented diet

Tirnitz–Parker, Janina E.E.; Tonkin, Joanne; Knight, Belinda; Olynyk, John K.; Yeoh, George

doi:10.1016/j.biocel.2007.06.008

Cited by 98 publications

(101 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The clonal LPC lines BMOL and BMOL-TAT 18 were confirmed to be strongly positive for the TWEAK receptor Fn14 in the majority of cells (Fig. 5A).…”

Section: Resultsmentioning

confidence: 78%

“…We have previously established immortalized LPC lines from WT or transgenic CDE-treated mouse livers and characterized as bipotential murine oval liver (BMOL) cells. 18 As early as 24 hours after TWEAK exposure, growth of the BMOL lines was notably stimulated; this continued for up to 6 days with no plateau apparent. Proliferation was dose-dependent up to 50 ng/mL rhTWEAK; at 100 ng/mL, however, growth was slightly reduced, as was NFjB activation.…”

Section: Discussionmentioning

confidence: 96%

“…Livers of 2-week-CDEtreated mice were perfused, digested, and enriched for LPCs by discontinuous Percoll gradient centrifugation, as described previously. 18 Cell Lines and Cell Culture. The murine, bipotential LPC lines-bipotential murine oval liver (BMOL) and bipotential murine oval liver-tyrosine aminotransferase (BMOL-TAT)-were established from CDEtreated livers.…”

Section: Methodsmentioning

confidence: 99%

“…The murine, bipotential LPC lines-bipotential murine oval liver (BMOL) and bipotential murine oval liver-tyrosine aminotransferase (BMOL-TAT)-were established from CDEtreated livers. 18 Both lines were maintained as previously reported unless otherwise indicated. For proliferation experiments, serum and growth factor concentrations in the media were reduced to 2% fetal bovine serum (FBS), 15 ng/mL insulin-like growth factor II (IGF-II), 10 ng/mL epidermal growth factor (EGF), and 5 lg/mL insulin for three consecutive passages prior to use.…”

Section: Methodsmentioning

confidence: 99%

“…Immunofluorescence was performed on methanol/acetone-fixed liver cryosections, cytospins of BMOL cell lines, or primary LPC isolates 18 according to a standard protocol with antibodies diluted in REAL antibody diluent (Dako, Denmark), and mounting was accomplished with the ProLong Gold antifade reagent with 4 0 ,6-diamidino-2-phenylindole (DAPI; Invitrogen) for nuclear quantification. The primary antibodies were rat anti-A6 Sirius Red Staining.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Tumor necrosis factor-like weak inducer of apoptosis is a mitogen for liver progenitor cells

Tirnitz–Parker¹,

Viebahn²,

Jakubowski³

et al. 2010

Hepatology

Self Cite

157

182

View full text Add to dashboard Cite

“…The clonal LPC lines BMOL and BMOL-TAT 18 were confirmed to be strongly positive for the TWEAK receptor Fn14 in the majority of cells (Fig. 5A).…”

Section: Resultsmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Tumor necrosis factor-like weak inducer of apoptosis is a mitogen for liver progenitor cells

Tirnitz–Parker¹,

Viebahn²,

Jakubowski³

et al. 2010

Hepatology

Self Cite

157

182

View full text Add to dashboard Cite

Tumor‐Initiating Stem‐Like Cells

Machida

2014

Cancer Stem Cells

View full text Add to dashboard Cite

The “mini‐crush” technique for managing bifurcation lesions

Kornowski

2009

Cathet Cardio Intervent

View full text Add to dashboard Cite

The ''mini-crush'' is one of various techniques used to treat atherosclerotic coronary bifurcation lesions. This method was designed primarily to treat complex bifurcation lesions in cases where atherosclerotic disease involves both the side branch (SB) and the main branch (MB) [1]. The ''mini-crush'' is a ''two-stent'' technique that represents an alternative to a provisional ''single stent'' approach to catheter-based management of bifurcation lesions [2]. The provisional technique involves single stent placement in the MB with the option to add another stent in the SB according to intra-procedural need (e.g., plaque shift, SB dissection and/or abrupt closure, flow limitation, etc.) [3]. In catheter-based bifurcation management, key goals are to optimize the angioplasty outcome and assure long-term bifurcation patency without making the procedure unduly complicated from a technical standpoint. These goals can be accomplished using either a provisional single-stent approach with an optional two-stent bailout strategy or an a priori planned two-stent technique with one of several stenting options, such as the T or Y techniques or using the ''mini-crush'' strategy. About one-third of patients will require a twostent strategy [4].The ''mini crush'' technique was designed to provide complete coverage of the ostium of the SB while minimizing the length of the crushed segment [5]. In the ''mini-crush'' paradigm, both the MB and the SB are wired and pre-dilated using balloons. A 7 or even 8 French guiding catheter is recommended to allow a large space for catheter equipment delivery. Next, one stent each is delivered to the SB and the MB, in that sequence. The SB stent is brought to the midline crossing point of the MB stent, using the proximal marker of the SB stent as an indicator to assure a contact and even minimal overlap with the MB stent. This short overlapping segment will later become the SB ''mini crushed'' segment. When both of the stents have been appropriately positioned, the SB stent should be deployed at nominal or even supranominal pressure. Then, the delivery balloon of the SB stent is drawn backward and an additional act of 'high pressure' dilatation of the SB ostium is recommended. This maneuver should assure optimal apposition and even some flaring of the stent in the SB ostium, and prevent narrowing or separation of the stent during subsequent MB stent dilatation. Then, the SB wire is removed in order to prevent irreversible ''jailing'' of the wire, and the MB stent is dilated and deployed across the SB at a nominal or even higher pressure (i.e. pending the distal reference diameter).Next, the guide wire is reinserted into the SB ostium in order to introduce a low-profile balloon through the crushed segment. Recrossing of the SB ostium using a wire and/or balloon can be technically challenging. This step will allow for subsequent redilation of the bifurcation carina and the SB ostium. Initially, the SB and MB stents can be dilated separately at a relatively high-pressure followed by a final simulta...

show abstract

Isolation, culture and immortalisation of hepatic oval cells from adult mice fed a choline-deficient, ethionine-supplemented diet

Cited by 98 publications

References 38 publications

Tumor necrosis factor-like weak inducer of apoptosis is a mitogen for liver progenitor cells

Tumor necrosis factor-like weak inducer of apoptosis is a mitogen for liver progenitor cells

Tumor‐Initiating Stem‐Like Cells

The “mini‐crush” technique for managing bifurcation lesions

Contact Info

Product

Resources

About