Isolation, chemical characteristics and antioxidant properties of the polysaccharides from marine fungus Penicillium sp. F23-2

Sun, He; Mao, Wenjun; Chen, Yin; Guo, Shoudong; Li, Hongyan; Qi, Xiaohui; Chen, Yanli; Xu, Jian

doi:10.1016/j.carbpol.2009.04.017

Cited by 160 publications

(71 citation statements)

References 33 publications

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“…The hydroxyl radical is the strongest reactive free radical among reactive oxygen species, and it can react with all bio-macromolecules in living cells (Huang et al, 2005;Sun et al, 2009). However no enzymes found in organisms are known to degrade the hydroxyl radical (Akashi et al, 2004).…”

Section: Scavenging Of Hydroxyl Radical By Pectin Treated By Diff Erementioning

confidence: 99%

Optimization of pectin extraction and antioxidant activities from Jerusalem artichoke

Xue-jie

et al. 2015

Chin. J. Ocean. Limnol.

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Jerusalem artichoke is an economic crop widely planted in saline-alkaline soil. The use of Jerusalem artichoke is of great signifi cance. In this study, the response surface method was employed to optimize the eff ects of processing variables (extraction temperature, pH, extraction time, and liquid-tosolid ratio) on the yield of Jerusalem artichoke pectin. Under the optimal extraction conditions: pH 1.52, 63.62 min, 100°C and a liquid-to-solid ratio of 44.4 mL/g, the maximum pectin yield was predicted to be 18.76%. Experiments were conducted under these optimal conditions and a pectin yield of 18.52±0.90% was obtained, which validated the model prediction. The eff ects of diff erent drying methods (freeze drying, spray drying and vacuum drying) on the properties of Jerusalem artichoke pectin were evaluated and they were compared with apple pectin. FTIR spectral analysis showed no major structural diff erences in Jerusalem artichoke pectin samples produced by various drying treatments. The antioxidant activities of pectin dried by diff erent methods were investigated using in vitro hydroxyl and DPPH radical scavenging systems. The results revealed that the activities of spray dried pectin (SDP) and apple pectin (AP) were stronger than those of vacuum oven dried pectin (ODP) and vacuum freeze dried pectin (FDP). Therefore compared with the other two drying methods, the spray drying method was the best.

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Section: Scavenging Of Hydroxyl Radical By Pectin Treated By Diff Erementioning

confidence: 99%

Optimization of pectin extraction and antioxidant activities from Jerusalem artichoke

Xue-jie

et al. 2015

Chin. J. Ocean. Limnol.

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“…Fw-1 had a higher scavenging ability on DPPH radicals than the extracellular polysaccharides PS2-1, PS1-2, and PS1-1 isolated from marine fungus Penicillium sp. F23-2 (EC 50 value 2.53-6.81 mg/mL) (Sun et al 2009). The present result suggested that the extracellular polysaccharide Fw-1 could be a potential antioxidant.…”

Section: Discussionmentioning

confidence: 99%

Preparation and Characterization of a Novel Extracellular Polysaccharide with Antioxidant Activity, from the Mangrove-Associated Fungus Fusarium oxysporum

et al. 2015

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Marine fungi are recognized as an abundant source of extracellular polysaccharides with novel structures. Mangrove fungi constitute the second largest ecological group of the marine fungi, and many of them are new or inadequately described species and may produce extracellular polysaccharides with novel functions and structures that could be explored as a source of useful polymers. The mangrove-associated fungus Fusarium oxysporum produces an extracellular polysaccharide, Fw-1, when grown in potato dextrose-agar medium. The homogeneous Fw-1 was isolated from the fermented broth by a combination of ethanol precipitation, ion-exchange, and gel filtration chromatography. Chemical and spectroscopic analyses, including one- and two-dimensional nuclear magnetic resonance spectroscopies showed that Fw-1 consisted of galactose, glucose, and mannose in a molar ratio of 1.33:1.33:1.00, and its molecular weight was about 61.2 kDa. The structure of Fw-1 contains a backbone of (1 → 6)-linked β-D-galactofuranose residues with multiple side chains. The branches consist of terminal α-D-glucopyranose residues, or short chains containing (1 → 2)-linked α-D-glucopyranose, (1 → 2)-linked β-D-mannopyranose, and terminal β-D-mannopyranose residues. The side chains are connected to C-2 of galactofuranose residues of backbone. The antioxidant activity of Fw-1 was evaluated with the scavenging abilities on hydroxyl, superoxide, and 1,1-diphenyl-2-picrylhydrazyl radicals in vitro, and the results indicated that Fw-1 possessed good antioxidant activity, especially the scavenging ability on hydroxyl radicals. The investigation demonstrated that Fw-1 is a novel galactofuranose-containing polysaccharide with different structural characteristics from extracellular polysaccharides from other marine microorganisms and could be a potential source of antioxidant.

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“…The modification method of Sun et al (2009) was carried out to quantitative determination of monosaccharide with reversedphase high performance liquid chromatography after pre-column derivatization. Two milligram polysaccharide with 2 mL trifluoroacetic acid (TFA) solution (2 M) was hydrolyzed at 120 • C for 4 h in a sealed-tube fulfilled with N 2 .…”

Section: Analysis Of Monosaccharide Compositionmentioning

confidence: 99%

Purification and antioxidant effect of novel fungal polysaccharides from the stroma of Cordyceps kyushuensis

Zhang

Yin

Han

et al. 2015

Industrial Crops and Products

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Isolation, chemical characteristics and antioxidant properties of the polysaccharides from marine fungus Penicillium sp. F23-2

Cited by 160 publications

References 33 publications

Optimization of pectin extraction and antioxidant activities from Jerusalem artichoke

Optimization of pectin extraction and antioxidant activities from Jerusalem artichoke

Preparation and Characterization of a Novel Extracellular Polysaccharide with Antioxidant Activity, from the Mangrove-Associated Fungus Fusarium oxysporum

Purification and antioxidant effect of novel fungal polysaccharides from the stroma of Cordyceps kyushuensis

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