Isolation, Characterization, Antioxidant, Antimicrobial and Cytotoxic Effect of Marine Actinomycete, Streptomyces Carpaticus MK-01, against Fish Pathogens

Dharaneedharan, Subramanian; Kim, Min-Sun; Kim, Dong‐Hwi; Heo, Moon‐Soo

doi:10.1590/1678-4324-2017160539

Cited by 17 publications

(19 citation statements)

References 22 publications

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“…Several reports revealed that the actinomycetes from different habitats were found to be a good source of antiviral and antibacterial compounds against viruses and bacteria of plants, animals and humans (43, 44, 45, 46, 47, 48, 49, 50, 51, 52). A bioactive compound from a marine actinomycete isolate, S. kaviengensis showed strong antiviral activity against equine encephalitis virus (53).…”

Section: Discussionmentioning

confidence: 99%

“…This compound (20 μg mL −1 ) effectively inhibited the replication of the FNV, and the viral titre was reduced from 4.3 to 2.45 log TCID50 mL −1 on treatment (16). (17) reported the antiviral activity of crude extract of marine Streptomyces carpaticus MK-01 isolated from seawater against viral hemorrhagic septicemia virus of fish. Based on the above studies, an attempt was made in the present study to screen a group of actinomycetes collected from different habitats for antiviral activity against WSSV of shrimp.…”

Section: Introductionmentioning

confidence: 99%

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Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Rajkumar¹,

Manimaran

Taju³

et al. 2018

Preprint

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35Actinomycetes isolates collected from different environments were screened for 36 antiviral activity against WSSV. One isolate designated as CAHSH-2 showed antiviral 37 activity against WSSV at the concentration of 0.2 mg per shrimp. The laboratory trial of 38 determining antiviral activity of ethyl acetate extract (EtOAcE) of CAHSH-2 against WSSV 39 was carried out 21 times since 2014. CAHSH-2 isolate which showed antiviral activity was 40 characterized and identified as Streptomyces ghanaensis like strain. Among the five fractions 41 obtained from EtOAcE of potential actinomycetes isolate, F1 was found to have strong 42 antiviral activity. The F1A and F1B sub-fractions from F1 fraction were subjected to GC-MS, 43 FTIR, 1 H and 13 C NMR analyses and, the compounds identified were di-n-octyl phthalate and 44 bis (2-methylheptyl) phthalate, respectively. Among these compounds, di-n-octyl phthalate 45 showed strong antiviral activity against WSSV. Molecular docking studies revealed that di-n-46 octyl phthalate was found to have high binding affinity with VP26 and VP28 proteins of 47 WSSV, whereas the bis (2-methylheptyl) phthalate showed low binding affinity with VP26 48 and VP28. The antiviral activity of EtOAcE of actinomycetes against WSSV was confirmed 49 by PCR, RT-PCR, Western blot and ELISA. The EA extract of active isolate was found to be 50 non-toxic to Artemia, post-larvae and adult Litopenaeus vannamei. 51 Importance 52 53White spot syndrome virus (WSSV) is an important shrimp viral pathogen and 54 responsible for huge economic loss to shrimp culture industry worldwide including India. The 55 global loss due to WSSV has been estimated about USD 10 billion and the loss continues at 56 the same extent even now. Various strategies have been followed to prevent or control 57 diseases of aquatic animals. In spite of various preventive and control strategies, WSSV has 58 been still persisting for more than two decades. No control strategies have so far been evolved 59 to put a break to WSSV. In this situation, an attempt was made in the present work to screen 60 some actinomycetes isolates for antiviral activity against WSSV. Among these isolates, one 61 isolate identified as Streptomyces ghanaensis like isolate CAHSH-2 showed activity against 62 WSSV. This article gives the information about the antiviral compound against WSSV and 63 the mechanism of viral inhibition. 64 65 66

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Rajkumar¹,

Manimaran

Taju³

et al. 2018

Preprint

View full text Add to dashboard Cite

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“…was reported previously from marine sediments. [30,31] Various potential secondary metabolites have been reported from the latter, announcing extensive antibacterial activity against Gram-positive fish pathogenic bacteria. [32] Exopolysaccharides (EPS) which exhibit cytotoxicity against human tumor cells of breast and colon cancer lines were reported from Streptomyces carpaticus as well.…”

Section: Isolation and Taxonomic Characterization Of The Actinobactermentioning

confidence: 99%

Viscosine: A new microbial flavonoid from marine‐derived, Streptomyces sp. RMS518F

Selim

Shaaban

Hamdy

et al. 2019

Vietnam Journal of Chemistry

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During the search for bioactive metabolites from marine sediment derived-actinomycetes, a new microbial flavonoid 6-hydroxykaempferol-3,6-dimethyl ether, viscosine (1) was isolated from the marine sediment derived-actinobacteria RMS518F strain. Structure of the compound (1) was determined by 1D-and 2D-NMR (1 and 2-Dimensional Nuclear Magnetic Resonance), mass spectrometry and comparison of these data with literature. Taxonomically, the strain was characterized as Streptomyces carpaticus on the bases of 16S rRNA gene sequence. Biologically, compound (1) exhibited moderate antimicrobial activity against the Gram-positive bacteria, Staphylococcus aureus ATCC6538-P; Bacillus cereus ATCC11778 and Saccharomyces cerevisiae ATCC9080 yeast. Compound (1)was also significantly active against the human colon carcinoma cell line (HCT-116) and the hepatocellular carcinoma cell line (Hep G2) with IC50 values of 73.4 and 62.8 μg/mL, respectively compared with the antineoplastic drug (Doxorubicin) (37.6 and 21.6 μg/mL), while no activity has been noticed against the human breast cancer cell line (MCF-7).

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“…Furthermore, in goal selection of Mediterranean Sea in present work as an isolation habitat was enhanced by the survey carried out by Tuncer & Bizsel et al (2017) who reported that there is a limited number of studies in sediments of Eastern Mediterranean Sea, especially in relation with environmental parameters, in spite of high morphological and phylogenetic diversity, biotechnological and economic importance of actinomycetes. A wide diversity of marine mesophilic actinomycetes were inspected for their bioactive compounds production (Eccleston et al, 2012;Orlova et al, 2015 andSubramanian et al, 2017), while thermophilic actinomycetes were not examined so far; accordingly, current investigation included them throughout. Maldonado et al, (2009) notified that previous works on marine sediments have been restricted to the isolation of members of the genera Micromonospora, Rhodococcus and Streptomyces while their study revealed that marine habitats are not restricted to these genera; this was found in parallel with our study findings which detected a member of genus Saccharomonospora as antimicrobial active potent isolate.…”

Section: Discussionmentioning

confidence: 99%

“…J. Microbiol. 52 (2017) SAHAR YASSIN IBRAHIM marine habitats (Valli , et al, 2012;Sengupta et al 2015 andSubramanian et al, 2017). The majority of these compounds demonstrate one or more bioactivities many of them developed into drugs for treatment of wide range of diseases in human, veterinary and agriculture sectors (Battershill et al, 2005 andKafilzadeh &Dehdari , 2015).…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification and Antibiosis Efficacy of Marine Thermophilic Actinomycetes

Ibrahim

2017

Egypt. J. Microbiol.

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T HIS WORK is aimed to assess the antibiosis efficacy of marine thermophilic actinomycetes isolated from raw marine habitat and identify the most antibiosis effective isolate. Variable thermophilic actinomycete strains were isolated from 14 marine sediment samples at Burj Al Arab, El-Agamy salinases and Cairo-Alex route Alexandria, Egypt. The isolated strains were purified and screened for their antibiosis activities against various targets of human pathogenic bacteria and fungi. Actinomycete isolate, characterized by highest broad-spectrum antagonism, was selected and identified based on morphological, cultural, physiological and biochemical characteristics. Total cell hydrolysate analysis, Genomic DNA extraction and 16S rRNA sequencing were also characterized. Among the purified 135 marine thermophilic actinomycete isolates, twenty strains showed detectable antagonistic activities against test pathogens. Based on identification collected data, it was suggested that the most potent undertreat isolate is belonging to actinomycete genus Saccharomonospora. Molecular studies revealed that it was highly related to Saccharomonospora viridis (100%). As a result, it was designated as Saccharomonospora viridis AHK190.

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Isolation, Characterization, Antioxidant, Antimicrobial and Cytotoxic Effect of Marine Actinomycete, Streptomyces Carpaticus MK-01, against Fish Pathogens

Cited by 17 publications

References 22 publications

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Viscosine: A new microbial flavonoid from marine‐derived, Streptomyces sp. RMS518F

Isolation, Identification and Antibiosis Efficacy of Marine Thermophilic Actinomycetes

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