Isolation, characterization, and probiotic profiling of amylolytic lactic acid bacteria from Sonadi sheep milk

Abstract

Probiotics are live, healthy bacteria that improve health when given to a host in sufficient quantities. This research explored the possible probiotic attributes of amylase-producing lactic acid bacteria (ALAB) from milk of Sonadi sheep breed. Five strains (P1, P2, P3, P4, and P5) out of 76 isolates were found to be amylase-producing LAB, which was characterized by morphological, phenotyping, and biochemical tests and identified by 16S rRNA sequencing and phylogenetic evaluation. The strains were characterized for probiotic attributes such as tolerance to bile salt and acid, resistance to lysozyme and phenol, antioxidative activity, simulated gastric and pancreatic digestion, cell surface properties (auto-aggregation and cell surface hydrophobicity), antimicrobial activity (antifungal and antibacterial), and safety assessment (antibiotic susceptibility, hemolytic and DNase activities). This study exhibited that Lactiplantibacillus plantarum P2 and Enterococcus faecium P1 were observed to have excellent probiotic traits and could be a putative candidate as a probiotic for the manufacturing of fermented products.