Isolation, characterization, and interaction of lignin‐degrading bacteria from rumen of buffalo (<i>Bubalus bubalis</i>)

Wang, Zhen; Wu, Wenqing; Cui, Luncheng; Li, Xiang; Kulyar, Muhammad Fakhar‐e‐Alam; Xiong, Haiqian; Zhou, Nian E.; Yin, Huaihui; Li, Jiakui; Li, Xiang

doi:10.1002/jobm.202100068

Cited by 9 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, several microorganisms with lignin degradation potential were isolated from buffalo rumen with lignin degradation ability. This result is consistent with Wang's research which also isolated strains with lignin degradation potential in buffalo rumen [39]. The study seems to point out that rumen microorganisms were the underlying mechanisms of lignin degradation.…”

Section: Discussionsupporting

confidence: 92%

The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (Bubalus bubalis)

et al. 2021

Self Cite

View full text Add to dashboard Cite

The water buffalo (Bubalus bubalis) is known for its unique utilization of low-quality fibrous feeds and outstanding digestion performance, highlighting its role as an animal model in studying fiber fractions degradation. Among roughage, lignin attracted wide attention in ruminant nutrition studies, which affects animal digestibility. Therefore, the present study aims to investigate the functional relation between three lignin monomeric compositions of coniferyl alcohol (G), ρ-coumaryl alcohol (H) and sinapyl alcohol (S) and ruminal fiber degradation in water buffalo. Hence, three female water buffaloes (Nili-Ravi × Mediterranean, five years old, 480 ± 20 kg) were assigned for an in vivo study by utilizing the nylon-bag method, examining eight kinds of roughage. All the experimental roughage types were analyzed for the effective degradability (ED) of neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), cellulose (CEL) and hemicellulose (HC) fractions. Then, prediction models for the roughage fiber degradation were established based on the characteristics of lignin monomer content. The results showed that S, S/G and S/(G+S+H) were positively correlated with the ED of NDF, ADF, CEL and HC; H/S was negatively correlated. For the effective degradability of ADL (ADLD), S and S/(G+S+H) were positively correlated with it; H, H/G, H/S and H/(G+S+H) were negatively correlated. The model with the highest fitting degree was ADLD = 0.161 − 1.918 × H + 3.152 × S (R2 = 0.758, p < 0.01). These results indicated that the lignin monomer composition is closely related to the utilization rate of roughage fiber. S-type lignin monomer plays a vital role in the fiber degradation of roughage. The experiment found the effect of lignin monomer composition on the degradation of fiber fractions using buffalo as the experimental animal and constructed prediction models, providing a scientific basis for building a new technological method using lignin composition to evaluate buffalo roughage. Furthermore, the capacity of ADL degradation of buffalo was proved in this experiment. In order to further explore the ability of lignin degradation by the buffalo, the DNA of rumen microorganisms was extracted for sequencing. The top three composition of rumen microorganisms at the genus level were Prevotella_1, 226, Rikenellaceae_RC9_gut_group and Ruminococcaceae_UCG-011. Six strains with lignin degradation ability were screened from buffalo rumen contents. This experiment also revealed that the buffalos possess rumen microorganisms with lignin degradation potential.

show abstract

Section: Discussionsupporting

confidence: 92%

The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (Bubalus bubalis)

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…such as Bacillus sonorensis and Bacillus amyloliquefaciens have been also isolated during degrading RS. [25][26][27] The B. cereus strain W118 grown on a CMC-Na medium showed round, white and opaque colony (Fig. 2a).…”

Section: Resultsmentioning

confidence: 99%

Efficient degradation of rice straw through a novel psychrotolerant Bacillus cereus at low temperature

Wang

Cai

2022

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND: Rice straw (RS) is one of the largest sources of lignocellulosic, which is an abundant raw material for biofuels and chemicals. However, the natural degradation of RS under a low temperature environment is the biggest obstacle to returning straw to the field. RESULTS:In the present study, one bacillus strain W118 was isolated. Strain W118 was identified as Bacillus cereus through morphological and physiological characterization and 16S rDNA sequencing. The optimum growth temperature and pH of strain W118 were 20 °C and 6.5, respectively. Simultaneously, it was found that the strain W118 grew well at low temperature, even at a temperature of 4 °C (OD 600 = 1.40 ± 0.01). The decrease of various compositions of RS after the fermentation process at a temperature of 20 °C and 4 °C for 14 days was 27.00 ± 0.02% and 23.70 ± 0.04%, respectively. The composition of RS decreased to 50.71 ± 0.02% after being fermented at 4 °C for 25 days. The results of scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction of RS showed that the compositions of RS were significant decreased.CONCLUSION: This test suggests that the strain W118 is efficient for degrading RS at low temperature, which has great application potential for straw degradation in a low temperature area.

show abstract

“…Interestingly, buffalo was the first mammal to be verified to have the ability of lignin degradation (Xu et al, 2021 ). According to a prior study, strains with lignin degradation ability were isolated from buffalo rumen, and the degradation rate of sodium lignosulfonate could reach 11.1% (Wang et al, 2021 ). This suggests that rumen microbes play an important role in the buffalo's ability to exhibit lignin degradation.…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification of ligninolytic bacterium (Bacillus cereus) from buffalo (Bubalus bubalis) rumen and its effects on the fermentation quality, nutrient composition, and bacterial community of rape silage

et al. 2023

Self Cite

View full text Add to dashboard Cite

This study aimed to isolate and identify a ligninolytic bacterium from the rumen of buffalo (Bubalus bubalis) and investigate its effects as a silage additive for whole-plant rape. Three lignin-degradation strains were isolated from the buffalo rumen, with AH7-7 being chosen for further experiments. Strain AH7-7, with acid tolerance and a 51.4% survival rate at pH 4, was identified as Bacillus cereus. It exhibited a lignin-degradation rate of 20.5% after being inoculated in a lignin-degrading medium for 8 days. We divided the rape into four groups according to the various additive compositions to examine the fermentation quality, nutritional value, and bacterial community after ensiling: Bc group (inoculated with B. cereus AH7-7 3.0 × 106 CFU g FW−1), Blac group (inoculated with B. cereus AH7-7 1.0 × 106 CFU g FW−1, L. plantarum 1.0 × 106 CFU g FW−1, and L. buchneri 1.0 × 106 CFU g FW−1), Lac group (inoculated with L. plantarum 1.5 × 106 CFU g FW−1 and L. buchneri 1.5 × 106 CFU g FW−1), and Ctrl group (no additives). After 60 days of fermentation, the application of B. cereus AH7-7 was potent in modulating the fermentation quality of silage, especially when combined with L. plantarum and L. buchneri, as indicated by lower dry matter loss and higher contents of crude protein, water-soluble carbohydrate, and lactic acid. Furthermore, treatments with the B. cereus AH7-7 additive decreased the contents of acid detergent lignin, cellulose, and hemicellulose. The B. cereus AH7-7 additive treatments reduced the bacterial diversity and optimized the bacterial community compositions of silage, with an increase in the relative abundance of beneficial Lactobacillus and a decrease in the relative abundance of undesirable Pantoea and Erwinia. Functional prediction revealed that inoculation with B. cereus AH7-7 could increase the cofactors and vitamins metabolism, amino acid metabolism, translation, replication and repair, and nucleotide metabolism, while decreasing the carbohydrate metabolism, membrane transport, and energy metabolism. In brief, B. cereus AH7-7 improved the microbial community, fermentation activity, and ultimately the quality of silage. The ensiling with B. cereus AH7-7, L. plantarum, and L. buchneri combination is an effective and practical strategy to improve the fermentation and nutrition preservation of rape silage.

show abstract

Isolation, characterization, and interaction of lignin‐degrading bacteria from rumen of buffalo (Bubalus bubalis)

Cited by 9 publications

References 50 publications

The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (Bubalus bubalis)

The Effect of Lignin Composition on Ruminal Fiber Fractions Degradation from Different Roughage Sources in Water Buffalo (Bubalus bubalis)

Efficient degradation of rice straw through a novel psychrotolerant Bacillus cereus at low temperature

Isolation and identification of ligninolytic bacterium (Bacillus cereus) from buffalo (Bubalus bubalis) rumen and its effects on the fermentation quality, nutrient composition, and bacterial community of rape silage

Contact Info

Product

Resources

About