Isolation, characterization and identification of a Paracoccus sp. 2‐haloacid‐degrading bacterium from the marine sponge Hymeniacidon perlevis

Huang, Jing; Xin, Yanjuan; Zhang, Wei

doi:10.1002/jobm.201000205

Cited by 16 publications

(3 citation statements)

References 22 publications

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“…To date concerning haloalkanoic dehalogenases, only four marine L-2-HADs have been biochemically characterized: DehRhb from a Rhodobacteraceae bacterium associated to marine Polychaeta ( Novak et al, 2013a ); two enzymes from the sponge-associated Paracoccus sp. DEH99 and Pseudomonas stutzeri DEH130 ( Huang et al, 2011 ; Zhang et al, 2013 ); and one from the psychrophilic strain Psychromonas ingrahamii DehPsi ( Novak et al, 2013b ). The best-studied marine L-2-HAD so far is DehRhb, whose structure and catalytic properties notably differ from soil enzymes ( Novak et al, 2013a ).…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of a L-2-Haloacid Dehalogenase From Zobellia galactanivorans DsijT Suggests a Role in Haloacetic Acid Catabolism and a Wide Distribution in Marine Environments

et al. 2021

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L-2-halocid dehalogenases (L-2-HADs) have been mainly characterized from terrestrial polluted environments. By contrast, knowledge is still scarce about their role in detoxification of predominant halocarbons in marine environments. Here, phylogenetic analyses showed a wide diversity of homologous L-2-HADs, especially among those belonging to marine bacteria. Previously characterized terrestrial L-2-HADs were part of a monophyletic group (named group A) including proteins of terrestrial and marine origin. Another branch (named group B) contained mostly marine L-2-HADs, with two distinct clades of Bacteroidetes homologs, closely linked to Proteobacteria ones. This study further focused on the characterization of the only L-2-HAD from the flavobacterium Zobellia galactanivorans DsijT (ZgHAD), belonging to one of these Group B clades. The recombinant ZgHAD was shown to dehalogenate bromo- and iodoacetic acids, and gene knockout in Z. galactanivorans revealed a direct role of ZgHAD in tolerance against both haloacetic acids. Analyses of metagenomic and metatranscriptomic datasets confirmed that L-2-HADs from group A were well-represented in terrestrial and marine bacteria, whereas ZgHAD homologs (group B L-2-HADs) were mainly present in marine bacteria, and particularly in host-associated species. Our results suggest that ZgHAD homologs could be key enzymes for marine Bacteroidetes, by conferring selective advantage for the recycling of toxic halogen compounds produced in particular marine habitats, and especially during interactions with macroalgae.

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Section: Introductionmentioning

confidence: 99%

Functional Characterization of a L-2-Haloacid Dehalogenase From Zobellia galactanivorans DsijT Suggests a Role in Haloacetic Acid Catabolism and a Wide Distribution in Marine Environments

et al. 2021

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“…Enrichment medium supplemented with 2-chloropropionic acid have been adapted in the combined qualitative pH indicator and quantitative HPLC method in the isolation of Paracoccus Sp. DEH99 2-haloacid dehalogenase-producing bacteria [ 216 ].…”

Section: Introductionmentioning

confidence: 99%

Dehalogenases: From Improved Performance to Potential Microbial Dehalogenation Applications

et al. 2018

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The variety of halogenated substances and their derivatives widely used as pesticides, herbicides and other industrial products is of great concern due to the hazardous nature of these compounds owing to their toxicity, and persistent environmental pollution. Therefore, from the viewpoint of environmental technology, the need for environmentally relevant enzymes involved in biodegradation of these pollutants has received a great boost. One result of this great deal of attention has been the identification of environmentally relevant bacteria that produce hydrolytic dehalogenases—key enzymes which are considered cost-effective and eco-friendly in the removal and detoxification of these pollutants. These group of enzymes catalyzing the cleavage of the carbon-halogen bond of organohalogen compounds have potential applications in the chemical industry and bioremediation. The dehalogenases make use of fundamentally different strategies with a common mechanism to cleave carbon-halogen bonds whereby, an active-site carboxylate group attacks the substrate C atom bound to the halogen atom to form an ester intermediate and a halide ion with subsequent hydrolysis of the intermediate. Structurally, these dehalogenases have been characterized and shown to use substitution mechanisms that proceed via a covalent aspartyl intermediate. More so, the widest dehalogenation spectrum of electron acceptors tested with bacterial strains which could dehalogenate recalcitrant organohalides has further proven the versatility of bacterial dehalogenators to be considered when determining the fate of halogenated organics at contaminated sites. In this review, the general features of most widely studied bacterial dehalogenases, their structural properties, basis of the degradation of organohalides and their derivatives and how they have been improved for various applications is discussed.

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“…Degradation of herbicide Dalapon was reported earlier by Magee and Colmer [2] after observation of bacteria that produce dehalogenase enzyme. Since then, studies on isolation of microbes that potentially produce dehalogenase have been undertaken [3][4][5][6]. Several bacteria are known to be able to degrade these haloaliphatic pollutants and many of these microorganisms have been isolated from the soil contaminated area or some from marine environment [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of dehalogenase from bacteria isolated from the Gut of Pond-reared Rohu (Labeo rohita) Juveniles in Myanmar

Abel¹,

Pakingking²,

Gregoria³

et al. 2012

ABB

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Unwarranted accumulation of halogenated compounds in the rivers and streams has in recent years emerged due to the widespread use agricultural pesticides. The presence of these halogenated compounds in the water does not only suppress the immune system of fish but adversely induce serious morbidity and mortality among cultured stocks. Importantly, gradual accumulation of these compounds in the system of cultured and wild freshwater fish species cultured in ponds and floating net-cages in dams and rivers, respectively, poses some risks to humans, the end users. In this study, we attempted to isolate bacteria from the gut of pond-reared rohu (Labeo rohita) in Myanmar, screened the isolated bacteria for dehalogenase gene using molecular technique and tested the ability of these bacteria to degrade halogenated compounds in vitro. The eight bacterial strains studied were identified as Enterobacter mori strain MK- 121001, Enterobacter cloacae strains MK121003, MK-121004, MK121010, Ralstonia solanacearum strain 121002, Acinetobacter baumannii strain MK121007, Chromobacterium violaceum strain MK121009 and Pantoea vagans strain 121011. Only three bacterial strains (MK121002, MK121007 and MK121009) were capable of degrading 2,2-dichloropropionic acid (2,2-DCP) as the sole carbon source up to a final substrate concentration of 20 mM. Their mean growth doubling time ranging from 6-23 hours with the maximum of chloride ion released of 85%. PCR amplifica- tion with oligonucleotide primers designed from group I dehalogenase revealed the presence of deha- logenase genes in all isolates suggesting dehalogenase gene in strains 121001, 121003, 121004, 121010 and 121011 were silenced. In contrast, group II dehalogenase primers did not show any PCR amplification. These results suggest that MK121002, MK121007 and MK121009 only encode a group I dehalogenase and its non-stereoselectivity is in agreement with previoulsly described group I haloacid dehalogenase. The partial gene sequences were blasted but no significant sequence identity was observed. Therefore, it suggest the 2-haloacid dehalogenase of MK121002, MK12-1007 and MK121009 might be a novel group I 2-haloacid dehalogenase. The results indicated a broad distribution of dehalogenation genes in many micro- bial genomes that harbor dehalogenase(s) due to the exposure of the microorganisms to the naturally occurring or man-made halogenated compounds in the environmental systems. So far, microorganisms capable of producing dehalogenases were mainly isolated from soil and scarcely from aquatic animals and their environments. To the authors’ knowledge, this is the first report on the isolation of dehalogenase-producing bacteria from the gut of pond-reared freshwater fish, Labeo rohita, in Myanmar

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Isolation, characterization and identification of a Paracoccus sp. 2‐haloacid‐degrading bacterium from the marine sponge Hymeniacidon perlevis

Cited by 16 publications

References 22 publications

Functional Characterization of a L-2-Haloacid Dehalogenase From Zobellia galactanivorans DsijT Suggests a Role in Haloacetic Acid Catabolism and a Wide Distribution in Marine Environments

Functional Characterization of a L-2-Haloacid Dehalogenase From Zobellia galactanivorans DsijT Suggests a Role in Haloacetic Acid Catabolism and a Wide Distribution in Marine Environments

Dehalogenases: From Improved Performance to Potential Microbial Dehalogenation Applications

Characteristics of dehalogenase from bacteria isolated from the Gut of Pond-reared Rohu (<i>Labeo rohita</i>) Juveniles in Myanmar

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Isolation, characterization and identification of a Paracoccus sp. 2‐haloacid‐degrading bacterium from the marine sponge Hymeniacidon perlevis

Cited by 16 publications

References 22 publications

Functional Characterization of a L-2-Haloacid Dehalogenase From Zobellia galactanivorans DsijT Suggests a Role in Haloacetic Acid Catabolism and a Wide Distribution in Marine Environments

Functional Characterization of a L-2-Haloacid Dehalogenase From Zobellia galactanivorans DsijT Suggests a Role in Haloacetic Acid Catabolism and a Wide Distribution in Marine Environments

Dehalogenases: From Improved Performance to Potential Microbial Dehalogenation Applications

Characteristics of dehalogenase from bacteria isolated from the Gut of Pond-reared Rohu (&lt;i&gt;Labeo rohita&lt;/i&gt;) Juveniles in Myanmar

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Characteristics of dehalogenase from bacteria isolated from the Gut of Pond-reared Rohu (<i>Labeo rohita</i>) Juveniles in Myanmar