Isolation, Characterization andin vitroEvaluation of Specific Bacteriophages Targeting Extensive Drug Resistance Strains ofPseudomonas aeruginosaIsolated from Septic Burn Wounds

Abstract

Background Antibiotic resistant bacteria and various infections caused by them especially extensive drug resistance (XDR) strains and worrying statistics of mortality due to these strains and also the lack of a clear vision for development and production of new effective antibiotics have made the necessity of using alternative therapies more apparent. Materials and Methods In this study, specific phages affecting the Pseudomonas aeruginosa XDR strain were extracted from hospital wastewater and their laboratory characteristics along with lysis effect on 40 XDR strains of P. aeruginosa were investigated. Results The results indicated that three isolated phages (PaB1, PaBa2 and PaBa3) belonged to the Myoviridae and Pododoviridae families and were specific to Pseudomonas aeruginosa strains. More than 98% of phages absorbed their host in less than 10 minutes (Adsorption time <10 min) and completed their lytic cycle after 40 minutes (latent time = 40 min). Burst size of PaBa1, PaBa2 and PaBa3 was 240, 250 and 220 pfu/cell, respectively. PaBa1 lysed 62.5% of the XDR strains with the highest efficiency. The three Phage cocktail was effective against 67.5% of the studied strains. Conclusion The results of this study indicate the significant potential of these phages for therapeutic use and prophylaxis of infections caused by this bacterium.

Selection and characterization of bacteriophages specific to Salmonella Choleraesuis in swine

“…However, the phage cocktails were more effective at reducing all S. Choleraesuis strains than individual phages. Several studies have shown consistent findings [68][69][70][71]. Phage cocktails may remedy the problem of a narrow host range.…”

Genomic analysis of vB_PaS-HSN4 bacteriophage and its antibacterial activity (in vivo and in vitro) against Pseudomonas aeruginosa isolated from burn

“…Compare to the standard of care, the bacterial count was decreased and less serious adverse effects were observed in phage cocktail-treated individuals. Marashi et al 15 isolated PaBa1, PaBa2, and PaBa3 phages from the Myoviridae and Podoviridae families against XDR strains of P. aeruginosa , causing burn infections. Phage PaBa1 and the phage cocktail prepared from the three mentioned phages were able to lyse 62.5% and 67.5% of P. aeruginosa strains, respectively.…”