Isolation, characterization, and field evaluation of rice (Oryza sativa L., Var. IR64) mutants with altered responses to salt stress

Nakhoda, Babak; Leung, Hei; Mendioro, Merlyn S.; Mohammadi-Nejad, Ghasem

doi:10.1016/j.fcr.2011.11.004

Cited by 32 publications

(25 citation statements)

References 51 publications

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“…EMS induced rice mutants were used to investigate the complex mechanism involved in water and salt stress tolerance (Huang et al, 2009;Zhou et al, 2013). The morphological, physiological and proteomic characterization of some EMS induced mutants showed more tolerance to abiotic stresses for example salt and heat in contrast to their respective wild type lines (Ghaffari et al, 2014;Poli et al, 2013;Nakhoda et al, 2012;Mithra et al, 2016). However, there are no previous reports of screening rice mutants for tolerance to low P at field level and also under AWD conditions.…”

Section: Introductionmentioning

confidence: 99%

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Yugandhar¹,

Basava²,

Subrahmanyam³

et al. 2017

Aust J Crop Sci

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Mutants are powerful genetic resources in plant breeding and functional genomics studies. Sixty seven stable ethyl methane sulphonate (EMS) induced rice mutants and the wild type parent Nagina 22 (N22) were characterized for plant height, tiller number, panicle number and grain yield under normal, low P field and alternate wet and dry (AWD) conditions in the same season. They were also genotyped with 44 SSR markers and four Pup1 (Phosphorus uptake1) gene specific markers. Genetic diversity was analysed by combining phenotype and marker data using Ward-MLM method. Single marker analysis showed significant association of four markers RM19696, RM263, RM3688 and RM1942 with grain yield in all three conditions. K-1, a Pup1 gene specific marker was significantly associated with tiller number only under low P conditions. The average dissimilarity between mutants was 0.86 and cophenetic correlation coefficient was 0.74. Six mutants were selected as gain-of-function mutants as they showed significantly higher grain yield in all three conditions, compared with N22. The selected mutants are an important resource for gene discovery for enhanced tolerance to low P and water stress conditions and associated markers can be useful in marker assisted selection.

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Section: Introductionmentioning

confidence: 99%

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Yugandhar¹,

Basava²,

Subrahmanyam³

et al. 2017

Aust J Crop Sci

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“…The tolerant 'IR64' rice mutants had significantly lower SES scores (4.0 vs. 7.0) and better survival and growth than either IR64 or the sensitive mutants under saline fields [38]. Highly significant differences (P < 0.0001) were observed for all traits in ILs (Table 2) generation [10].…”

Section: Adaptation Of Saltol Ils To Salt Stress At Reproductive Stagmentioning

confidence: 91%

“…Likewise, a screening of 5000 M4 rice mutant lines from 'IR64' using the SES classification system led to identifying 935 mutant as tolerant; which were further advanced for screening at the reproductive stage. There were 35 and 8 mutants better than or as sensitive to IR64, respectively [38].…”

Section: Validation Of Saltol-ils At Vegetative Stagementioning

confidence: 99%

Improving salt tolerance of lowland rice cultivar ‘Rassi’ through marker-aided backcross breeding in West Africa

Bimpong¹,

Manneh²,

Sock³

et al. 2016

Plant Science

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Salt stress affects about 25% of the 4.4 million ha of irrigated and lowland systems for rice cultivation in West Africa (WA). A major quantitative trait locus (QTLs) on chromosome 1 (Saltol) that enhances tolerance to salt stress at the vegetative stage has enabled the use of marker-assisted selection (MAS) to develop salt-tolerant rice cultivar(s) in WA. We used 3 cycles of backcrossing with selection based on DNA markers and field-testing using 'FL478' as tolerant donor and the widely grown 'Rassi' as recurrent parent. In the BC3F2 stage, salt-tolerant lines with over 80% Rassi alleles except in the region around Saltol segment were selected. 429 introgression lines (Saltol-ILs) were identified as tolerant at vegetative stage, of which 116 were field-tested for four seasons at the reproductive stage. Sixteen Saltol-ILs had less yield loss (3-26% relative to control trials), and 8 Saltol-ILs showed high yield potential under stress and non-stress conditions. The 16 Saltol-ILs had been included for further African-wide testing prior to release in 6 WA countries. MAS reduced the time for germplasm improvement from at least 7 to about 4 years. Our objective is to combine different genes/QTLs conferring tolerance to stresses under one genetic background using MAS.

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“…In addition to conventional breeding, lines with enhanced saline tolerance have been generated by mutation. Nakhoda et al (2012) identified four tolerant rice mutant lines from about 5,000 diepoxybutane M 4 mutant lines using hydroponic screening at the seedling stage. These salt-tolerant rice mutants produced a yield that was comparable to that produced by the IR64 variety when they were grown under normal conditions, but produced an average of approximately 0.8 − 1.0 t ha − 1 greater yield than IR64 when the two were grown in saline fields (Nakhoda et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Nakhoda et al (2012) identified four tolerant rice mutant lines from about 5,000 diepoxybutane M 4 mutant lines using hydroponic screening at the seedling stage. These salt-tolerant rice mutants produced a yield that was comparable to that produced by the IR64 variety when they were grown under normal conditions, but produced an average of approximately 0.8 − 1.0 t ha − 1 greater yield than IR64 when the two were grown in saline fields (Nakhoda et al, 2012). Salt-tolerant mutant lines have also been acquired by using gamma irradiation to induce mutation and combining it with in vitro culture (Lang and Buu, 2008 (Till et al, 2007;Suzuki et al, 2008;Dierking and Bilyeu, 2009;Minoia et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Identification of gamma ray irradiation-induced mutations in membrane transport genes in a rice population by TILLING

et al. 2016

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A high-salt environment represents environmental stress for most plants. Those that can grow and thrive in such an environment must have membrane transport systems that can respond effectively. Plant roots absorb Na + from the soil, and the plant must maintain Na + homeostasis to survive salt stress. A major mechanism by which salt-tolerant plants adapt to salt stress is through modulation of ion transport genes. We have subjected a population of rice plants to mutagenesis, and identified lines with both single-nucleotide polymorphisms (SNPs) in membrane transport genes and altered responses to salt stress. Primers labeled with FAM or HEX fluorescent dyes were designed for nine target genes encoding membrane transport proteins that are believed to regulate salt stress tolerance. A TILLING (Targeting Induced Local Lesions IN Genome) assay was performed on 2,961 M 2 rice mutant lines using electrophoresis. After the TILLING assay, a total of 41 mutant lines containing SNPs in the target genes were identified and screened. The average number of mutations per gene was 1/492 kb in lines having SNPs, and the percentage of mutation sites per total sequence was 0.67. Among the 41 lines, nine had altered sequences in the exon region of the genes. Of these nine lines, seven were tolerant to salt stress after exposure to 170 mM NaCl for three weeks, while the other two lines were not more salt-tolerant than the control lines. Furthermore, five mutant lines containing SNPs in the coding region of OsAKT1, OsHKT6, OsNSCC2, OsHAK11 and OsSOS1 showed changed expression levels for each gene. We conclude that variation in membrane transport genes, such as expression levels and protein structures, may affect the rice plant's tolerance to salt stress. These mutations represent traits that may be selected for large rice mutant populations, permitting efficient acquisition of salt-tolerant lines.

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Isolation, characterization, and field evaluation of rice (Oryza sativa L., Var. IR64) mutants with altered responses to salt stress

Cited by 32 publications

References 51 publications

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Improving salt tolerance of lowland rice cultivar ‘Rassi’ through marker-aided backcross breeding in West Africa

Identification of gamma ray irradiation-induced mutations in membrane transport genes in a rice population by TILLING

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