Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Yadav, Santosh Kumar; Mishra, Paras Kumar

doi:10.3791/58448-v

Cited by 2 publications

(3 citation statements)

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“…64 On the other hand, the complete lack of Nanog and Octa4 mRNA expression allows us to rule out the option of stem cell pluripotency. 101,102 Alternatively, the Kit mRNA positive cells could represent the so-called telocytes, which are Cajal-like cells that have been described in human and murine hearts. 52 Telocytes co-express Kit, CD34, and PDGFRB and seem to play an active role in the progression of cardiac interstitial remodeling.…”

Section: Discussionmentioning

confidence: 99%

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Feline hypertrophic cardiomyopathy: reduced microvascular density and involvement of CD34+ interstitial cells

et al. 2021

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The sequence of pathological events in feline hypertrophic cardiomyopathy (fHCM) is still largely unknown, although we know that fHCM is characterized by interstitial remodeling in a macrophage-driven pro-inflammatory environment and that myocardial ischemia might contribute to its progression. This study aimed to gain further insights into the structural changes associated with interstitial remodeling in fHCM with special focus on the myocardial microvasculature and the phenotype of the interstitial cells. Twenty-eight hearts (16 hearts with fHCM and 12 without cardiac disease) were evaluated in the current study, with immunohistochemistry, RNA-in situ hybridization, and transmission electron microscopy. Morphometrical evaluations revealed a statistically significant lower microvascular density in fHCM. This was associated with structural alterations in capillaries that go along with a widening of the interstitium due to the accumulation of edema fluid, collagen fibers, and mononuclear cells that also proliferated locally. The interstitial cells were mainly of fibroblastic or vascular phenotype, with a substantial contribution of predominantly resident macrophages. A large proportion expressed CD34 mRNA, which suggests a progenitor cell potential. Our results indicate that microvascular alterations are key events in the pathogenesis of fHCM and that myocardial interstitial cell populations with CD34+ phenotype play a role in the pathogenesis of the disease.

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Section: Discussionmentioning

confidence: 99%

“…70 Myocardium: cardiac stem cell marker in rat and mouse. 59,101 Cats: identified in amniotic epithelial cells and mesenchymal stem cells; used as transcription factor to generate induced pluripotent stem cells. 20,46,87,105 Oct4 Transcription factor expressed by embryonic stem cells to maintain cell pluripotency.…”

Section: Mef2cmentioning

confidence: 99%

Feline hypertrophic cardiomyopathy: reduced microvascular density and involvement of CD34+ interstitial cells

et al. 2021

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“…In turn, Guo et al [93] briefly presented the regulatory factors that induce BMSCs to differentiate into CMCs. There are also studies showing successful rat/mouse CSC differentiation into CMCs using 5-aza [94,95]. Interesting results were obtained using extremely low-frequency electromagnetic fields, tuned at the Ca 2+ ion cyclotron energy resonance (Ca 2+ -ICR), to drive CSCs differentiation toward a cardiac-specific phenotype [96].…”

Section: Differentiation Potential Of Mscs: Stem Cell-based Therapymentioning

confidence: 99%

Stem Cell-Based Therapy and Cell-Free Therapy as an Alternative Approach for Cardiac Regeneration

Deszcz

2023

Stem Cells International

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The World Health Organization reports that cardiovascular diseases (CVDs) represent 32% of all global deaths. The ineffectiveness of conventional therapies in CVDs encourages the development of novel, minimally invasive therapeutic strategies for the healing and regeneration of damaged tissue. The self-renewal capacity, multilineage differentiation, lack of immunogenicity, and immunosuppressive properties of mesenchymal stem cells (MSCs) make them a promising option for CVDs. However, growing evidence suggests that myocardial regeneration occurs through paracrine factors and extracellular vesicle (EV) secretion, rather than through differentiation into cardiomyocytes. Research shows that stem cells secrete or surface-shed into their culture media various cytokines, chemokines, growth factors, anti-inflammatory factors, and EVs, which constitute an MSC-conditioned medium (MSC-CM) or the secretome. The use of MSC-CM enhances cardiac repair through resident heart cell differentiation, proliferation, scar mass reduction, a decrease in infarct wall thickness, and cardiac function improvement comparable to MSCs without their side effects. This review highlights the limitations and benefits of therapies based on stem cells and their secretome as an innovative treatment of CVDs.

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Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Cited by 2 publications

References 0 publications

Feline hypertrophic cardiomyopathy: reduced microvascular density and involvement of CD34+ interstitial cells

Feline hypertrophic cardiomyopathy: reduced microvascular density and involvement of CD34+ interstitial cells

Stem Cell-Based Therapy and Cell-Free Therapy as an Alternative Approach for Cardiac Regeneration

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