Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs)

Ennis, Jane; Sarugaser, Rahul; Gomez, Alvaro; Baksh, Dolores; Davies, John E.

doi:10.1016/s0091-679x(08)00007-1

Cited by 31 publications

(23 citation statements)

References 30 publications

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“…47 It has recently been reported that MSCs can be isolated from the Wharton’s Jelly found in the umbilical cord (UC-MSC), and they are more abundant, more easily expanded and more resistant to cryogenic storage. 48 The adhesion, migration into scaffold, growth, spreading, osteogenic differentiation, ECM degradation and synthesis of BM-MSCs versus UC-MSCs was compared. The tested collagen scaffold showed excellent cytocompatibility with both cell types and could maintain high proliferation and viability.…”

Section: Methodsmentioning

confidence: 99%

Scaffold Design for Bone Regeneration

Polo-Corrales¹,

Latorre-Esteves²,

Ramı́rez-Vick³

2014

j. nanosci. nanotech.

766

502

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The use of bone grafts is the standard to treat skeletal fractures, or to replace and regenerate lost bone, as demonstrated by the large number of bone graft procedures performed worldwide. The most common of these is the autograft, however, its use can lead to complications such as pain, infection, scarring, blood loss, and donor-site morbidity. The alternative is allografts, but they lack the osteoactive capacity of autografts and carry the risk of carrying infectious agents or immune rejection. Other approaches, such as the bone graft substitutes, have focused on improving the efficacy of bone grafts or other scaffolds by incorporating bone progenitor cells and growth factors to stimulate cells. An ideal bone graft or scaffold should be made of biomaterials that imitate the structure and properties of natural bone ECM, include osteoprogenitor cells and provide all the necessary environmental cues found in natural bone. However, creating living tissue constructs that are structurally, functionally and mechanically comparable to the natural bone has been a challenge so far. This focus of this review is on the evolution of these scaffolds as bone graft substitutes in the process of recreating the bone tissue microenvironment, including biochemical and biophysical cues.

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Section: Methodsmentioning

confidence: 99%

Scaffold Design for Bone Regeneration

Polo-Corrales¹,

Latorre-Esteves²,

Ramı́rez-Vick³

2014

j. nanosci. nanotech.

766

502

View full text Add to dashboard Cite

show abstract

“…MSCs were obtained from the bone marrow of healthy donors (Davies et al., 2001), from umbilical perivasculature (Ennis et al., 2008) and from adipose tissue (Vermette et al., 2007). Tissue sections of mesenchymal tumors and respective healthy control tissues were purchased from Biomax.…”

Section: Methodsmentioning

confidence: 99%

Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells

et al. 2015

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SummaryPro-fibrotic microenvironments of scars and tumors characterized by increased stiffness stimulate mesenchymal stromal cells (MSCs) to express α-smooth muscle actin (α-SMA). We investigated whether incorporation of α-SMA into contractile stress fibers regulates human MSC fate. Sorted α-SMA-positive MSCs exhibited high contractile activity, low clonogenicity, and differentiation potential limited to osteogenesis. Knockdown of α-SMA was sufficient to restore clonogenicity and adipogenesis in MSCs. Conversely, α-SMA overexpression induced YAP translocation to the nucleus and reduced the high clonogenicity and adipogenic potential of α-SMA-negative MSCs. Inhibition of YAP rescued the decreased adipogenic differentiation potential induced by α-SMA, establishing a mechanistic link between matrix stiffness, α-SMA, YAP, and MSC differentiation. Consistent with in vitro findings, nuclear localization of YAP was positively correlated in α-SMA expressing stromal cells of adiposarcoma and osteosarcoma. We propose that α-SMA mediated contraction plays a critical role in mechanically regulating MSC fate by controlling YAP/TAZ activation.

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“…With respect to MSC isolation from umbilical cord, the different parts of this tissue should also be considered individually [35,36]. MSC can be isolated from whole umbilical cord [37], from Wharton's jelly (WJ-MSC) [38-40] or from umbilical cord blood (CB-MSC), [41,42] which also harbors hematopoietic stem cells, endothelial precursor cells and endothelial colony-forming cells (Figure 1).…”

Section: Msc Derived From Birth-associated Tissuesmentioning

confidence: 99%

Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC

et al. 2011

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The mesenchymal stroma harbors an important population of cells that possess stem cell-like characteristics including self renewal and differentiation capacities and can be derived from a variety of different sources. These multipotent mesenchymal stem cells (MSC) can be found in nearly all tissues and are mostly located in perivascular niches. MSC have migratory abilities and can secrete protective factors and act as a primary matrix for tissue regeneration during inflammation, tissue injuries and certain cancers.These functions underlie the important physiological roles of MSC and underscore a significant potential for the clinical use of distinct populations from the various tissues. MSC derived from different adult (adipose tissue, peripheral blood, bone marrow) and neonatal tissues (particular parts of the placenta and umbilical cord) are therefore compared in this mini-review with respect to their cell biological properties, surface marker expression and proliferative capacities. In addition, several MSC functions including in vitro and in vivo differentiation capacities within a variety of lineages and immune-modulatory properties are highlighted. Differences in the extracellular milieu such as the presence of interacting neighbouring cell populations, exposure to proteases or a hypoxic microenvironment contribute to functional developments within MSC populations originating from different tissues, and intracellular conditions such as the expression levels of certain micro RNAs can additionally balance MSC function and fate.

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Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs)

Cited by 31 publications

References 30 publications

Scaffold Design for Bone Regeneration

Scaffold Design for Bone Regeneration

Expression of α-Smooth Muscle Actin Determines the Fate of Mesenchymal Stromal Cells

Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC

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