Isolation, characterization and comparison of lytic Epseptimavirus phages targeting Salmonella

Cong, Cong; Wei, Bai; Cui, Huijing; Li, Xiaoyü; Yuan, Yuyu; Wang, Lili; Li, Shuying; Xu, Yongping

doi:10.1016/j.foodres.2021.110480

Cited by 16 publications

(3 citation statements)

References 80 publications

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“…As shown in Figure 4B , the GRNsp8 genome (≈111 Kbp) was approximately 2.5 times larger than that of GRNsp6 and its GC content (≈40%) was similar to that of representative phages from the Epseptimavirus genus ( Cong et al, 2021 ). The GRNsp8 genome contained a total of 191 putative ORFs, among which 65 ORFs had annotated functions, as follows: 22 ORFs encoding structural proteins, 39 ORFs encoding proteins involved in nucleotide metabolism, DNA replication/repair/transcription, and 4 ORFs encoding cell lysis proteins.…”

Section: Resultsmentioning

confidence: 92%

A Cocktail of Three Virulent Phages Controls Multidrug-Resistant Salmonella Enteritidis Infection in Poultry

Lv²,

Shi³

et al. 2022

Front. Microbiol.

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Salmonella enterica is not only the most common pathogen of poultry and poultry-derived products but is also a significant foodborne pathogen. In recent years, many S. enterica isolates have exhibited multi-drug resistance, which places huge pressure on global economy and health. Since phages are an attractive alternative to biocontrol pathogens, we isolated a total of 15 Salmonella phages from sewage effluent, sediment, and chicken manure. The GRNsp1, GRNsp3, GRNsp6, GRNsp21, GRNsp27, GRNsp30, GRNsp50, and GRNsp51 phages exhibited a wide host range against S. enterica serovars Enteritidis and Typhimurium in vitro. In particular, GRNsp51 exerted highly efficient lytic effects against a large proportion of S. Enteritidis and S. Typhimurium strains isolated from different regions of China. Meanwhile, GRNsp8 expanded the host range of GRNsp6 and GRNsp51. Based on their host ranges and lytic capacities, GRNsp6, GRNssp8, and GRNsp51 were selected for further investigation. Morphology, one-step growth curves, and stability assays revealed that GRNsp6, GRNsp8, and GRNsp51 all belong to the Caudovirales order and display relatively short latency periods with broad pH and thermal stability. Genomic analysis indicated that the genomes of these three phages contained no genes related to virulence, antibiotic resistance, or lysogeny. In addition, we tested the effectiveness of a cocktail composed of these three phages against S. Enteritidis in a chicken model. Treatment with the oral phage cocktail 24 h before or alongside Salmonella challenge significantly reduced colonization of the intestinal tract and decreased the mRNA expression of IL-6, IFN-γ, and IL-1β in the duodenum. Together, these findings indicate that a cocktail of the GRNsp6, GRNsp8, and GRNsp51 phages could serve as an effective antimicrobial therapeutic agent against multidrug-resistant Salmonella in animal production to mitigate infections by multiple zoonotic Salmonella species.

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Section: Resultsmentioning

confidence: 92%

A Cocktail of Three Virulent Phages Controls Multidrug-Resistant Salmonella Enteritidis Infection in Poultry

Lv²,

Shi³

et al. 2022

Front. Microbiol.

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“…As effective alternative antimicrobials for controlling bacterial contamination, phages are increasingly applied in the field of food industry, and some phage products are used as approved by Food and Drug Administration (FDA) ( Pinto et al, 2020 ; Ramos-Vivas et al, 2021 ). Phages can effectively reduce the bacterial load in various food matrices ( Han et al, 2013 ; Cong et al, 2021 ). Moreover, phages can be applied to the decontamination of food processing facility surfaces to prevent bacterial contamination ( Lewis and Hill, 2020 ; Federici et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila

Hou

Ren

et al. 2023

Front. Microbiol.

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Aeromonas hydrophila is an emerging foodborne pathogen causing human gastroenteritis. Aeromonas species isolated from food such as seafood presented multidrug-resistance (MDR), raising serious concerns regarding food safety and public health. The use of phages to infect bacteria is a defense against drug-resistant pathogens. In this study, phage ZPAH34 isolated from the lake sample exerted lytic activity against MDR A. hydrophila strain ZYAH75 and inhibited the biofilm on different food-contacting surfaces. ZPAH34 has a large dsDNA genome of 234 kb which belongs to a novel jumbo phage. However, its particle size is the smallest of known jumbo phages so far. Based on phylogenetic analysis, ZPAH34 was used to establish a new genus Chaoshanvirus. Biological characterization revealed that ZPAH34 exhibited wide environmental tolerance, and a high rapid adsorb and reproductive capacity. Food biocontrol experiments demonstrated that ZPAH34 reduces the viable count of A. hydrophila on fish fillets (2.31 log) and lettuce (3.28 log) with potential bactericidal effects. This study isolated and characterized jumbo phage ZPAH34 not only enriched the understanding of phage biological entity diversity and evolution because of its minimal virion size with large genome but also was the first usage of jumbo phage in food safety to eliminate A. hydrophila.

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“…Thus, a thorough genomic analysis of the bacteriophages is necessary to ensure the safety of the biocontrol agent for use in foods . Bacteriophages are promising biocontrol agents if they are not able to promote horizontal gene transfers (Cong et al, 2021). This study aimed to determine the morphological, biological, and genetic characteristics of lytic bacteriophages isolated from environmental samples, and to evaluate their biocontrol abilities on E. coli O157:H7 in pasteurized milk, ground beef and spinach.…”

Section: Introductionmentioning

confidence: 99%

Development of antimicrobial composite materials for food packaging applications

Choo¹

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Due to increasing environmental concerns regarding plastic waste, the development of composite film materials from biodegradable polymers and natural food additives is gaining more attention. Over the last few decades, the safety of synthetic additives that are commonly added as food additives has also been questioned because of their potential toxicity, carcinogenicity and teratogenicity effects. Natural antimicrobial agent could be a promising and versatile component in food packaging materials to improve their physiochemical, antioxidant and antimicrobial properties and hence, improve food quality and shelf life. In this study, novel chitosan/acetylated starch and polyvinyl alcohol/chitosan composite films were developed via a solution casting technique. In addition, a novel Escherichia phage, CAM-21, was isolated from a slurry lagoon at a local dairy farm before incorporating into soy protein isolate-based films. The results indicate that the chitosan/acetylated starch films incorporated with cinnamon and clove essential oils displayed enhanced light, water vapor, and oxygen barrier characteristics, as well as excellent antibacterial activity against spoilage bacteria and the pathogen, Escherichia coli O157:H7, on beef. Polyvinyl alcohol/chitosan films incorporated with aminosilane-modified bacterial nanocellulose and 4-hexylresorcinol exhibited improved mechanical, water vapor barrier, antioxidant, and antimicrobial properties against spoilage bacteria on vacuum packaged refrigerated raw beef. Also, CAM-21 has a broad host spectrum against various serotypes of Shiga toxin-producing E. coli. The growth of E. coli O157:H7 was effectively controlled in phage-treated milk, ground beef and baby spinach. Soy protein isolate films incorporated with CAM-21 showed excellent antimicrobial activity against E. coli O157:H7 in broth and refrigerated beef products. This project demonstrates the potential application of natural antimicrobial agents to produce novel composite films for antimicrobial food packaging.

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Isolation, characterization and comparison of lytic Epseptimavirus phages targeting Salmonella

Cited by 16 publications

References 80 publications

A Cocktail of Three Virulent Phages Controls Multidrug-Resistant Salmonella Enteritidis Infection in Poultry

A Cocktail of Three Virulent Phages Controls Multidrug-Resistant Salmonella Enteritidis Infection in Poultry

Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant Aeromonas hydrophila

Development of antimicrobial composite materials for food packaging applications

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