Isolation, characterization and chromatography based purification of antibacterial compound isolated from rare endophytic actinomycetes Micrococcus yunnanensis

Abstract

Endophytic actinomycetes are considered as one of the relatively unexplored potential sources in search of antibiotic producer against antibiotic resistant pathogens. A potent strain isolated from Catharanthus roseus that displays antibacterial potential against antibiotic resistant human pathogen Staphylococcus aureus was characterized and designated as Micrococcus yunnanensis strain rsk5. Rsk5 is capable of producing optimum antibacterial metabolites on starch casein medium at 30 °C, pH 5 and 2% NaCl condition. The crude antibacterial agent was extracted from fermentation broth by ethyl acetate and separated by TLC using chloroform-methanol (24:1, v/v) solvent system with Rf value of 0.26. It was partially purified by flash chromatography, followed by HPLC and analyzed by ultraviolet visible spectrophotometer to get absorption maxima at 208.4 nm. The ESI-MS spectra showed molecular ion peaks at m/z 472.4 [M-H], which does not match with any known antibacterial compound.

Isolation, characterization, anti-MRSA evaluation, and in-silico multi-target anti-microbial validations of actinomycin X2 and actinomycin D produced by novel Streptomyces smyrnaeus UKAQ_23

“…The purification of antimicrobial compounds was carried out by employing the preparative TLC plates (60 F254, 1000 µm, 20 × 20 cm, Miles Scientific, USA), size-exclusion chromatography (Sephadex LH-20; GE Healthcare Bio-Sciences AB, Uppsala, Sweden), and silica gel (60 Å, 0.071–0.16 mm, MACHEREY–NAGEL, Germany) column chromatography 54 , 81 , 82 .…”

Eco-friendly synthesis of silver nanoparticles by Bacillus subtilis and their antibacterial activity

“…Also, bacteria can survive in all kinds of unfavorable conditions, for instance, high or low peaks of temperatures, variable degrees of alkalinity or acidity, and high salt concentrations. At the same time, biologically formed nanoparticles have many applications, such as being catalysts in chemical reactions ( Li et al, 2016 ), optical receptors, an antimicrobial agent ( Ranjan and Jadeja, 2017 ), as well as being able to precipitate nanoparticles due to their metabolic activity. The biological synthesis of nanoparticles by bacteria is facilitated by their ability to precipitate those molecules out of cells, as those nanoparticles can be obtained using cell filtration which is considered beneficial for intracellular synthesis ( Natarajan and Selvaraj, 2010 ).…”

Investigating the Plant Growth Promoting and Biocontrol Potentiality of Endophytic Streptomyces sp. SP5 Against Early Blight in Tomato Seedling

“…Researcher isolated endophytic actinobacteria from different plants and are thought to be new sources of various bioactive compounds that can be used to ght pathogenic microorganisms (39)(40)(41)(42). Multiple reports have described the isolation of Streptomyces from plant roots followed by stems and leave exhibits antifungal and plant growth-promoting activities (43,44).…”