Isolation, characterization and application of a polyvalent phage capable of controlling Salmonella and Escherichia coli O157:H7 in different food matrices

Duc, Hoang Minh; Son, Hoang Minh; Yi, Hazel Pang Shu; Sato, Jun; Ngan, Pham Hong; Masuda, Yoshito; Honjoh, Ken Ichi; Miyamoto, Takahisa

doi:10.1016/j.foodres.2020.108977

Cited by 101 publications

(67 citation statements)

References 97 publications

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“…In the one-step growth curve result, the latent period of phage vB_SalP_TR2 was 15 min, and the average burst size was 211 PFU/cell. The latent period of phage vB_SalP_TR2 is shorter than that (25-65 min) of many other reported Salmonella phages (Huang et al, 2018;El-Dougdoug et al, 2019;Duc et al, 2020). The shorter latent period has been positively related to effectively inactivating bacteria (Li et al, 2020), indicating the utility of phage vB_SalP_TR2 in the biocontrol of Salmonella in practical settings.…”

Section: Discussionmentioning

confidence: 84%

“…Phage vB_SalP_TR2 could effectively inhibit the growth of S. Albany, with its performance in milk surpassing that in chicken meat. It is believed that this phenomenon is caused by the inability of the phages to move and reach the host on the surface of the solid food ( Guenther et al, 2012 ; Duc et al, 2020 ). The previously reported bacteriophages (PA13076 and LPST10) are useful in reducing the number of Salmonella in various food ingredients.…”

Section: Discussionmentioning

confidence: 99%

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Isolation and Characterization of a Novel Salmonella Phage vB_SalP_TR2

et al. 2021

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Salmonella is a widely distributed foodborne pathogen. The use of Salmonella phages as biocontrol agents has recently gained significant interest. Because the Salmonella genus has high diversity, efforts are necessary to identify lytic Salmonella phages focusing on different serovars. Here, five Salmonella phages were isolated from soil samples, and vB_SalP_TR2 was selected as a novel phage with high lytic potential against the host Salmonella serovar Albany, as well as other tested serovars, including Corvallis, Newport, Kottbus, and Istanbul. Morphological analyses demonstrated that phage vB_SalP_TR2 belongs to the Podoviridae family, with an icosahedral head (62 ± 0.5 nm in diameter and 60 ± 1 nm in length) and a short tail (35 ± 1 nm in length). The latent period and burst size of phage vB_SalP_TR2 was 15 min and 211 PFU/cell, respectively. It contained a linear dsDNA of 71,453 bp, and G + C content was 40.64%. Among 96 putative open reading frames detected, only 35 gene products were found in database searches, with no virulence or antibiotic resistance genes being identified. As a biological control agent, phage vB_SalP_TR2 exhibited a high temperature and pH tolerance. In vitro, it lysed most S. Albany after 24 h at 37°C with multiplicities of infection of 0.0001, 0.001, 0.01, 0.1, 1, 10, and 100. In food matrices (milk and chicken meat), treatment with phage vB_SalP_TR2 also reduced the number of S. Albany compared with that in controls. These findings highlighted phage vB_SalP_TR2 as a potential antibacterial agent for the control of Salmonella in food samples.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Novel Salmonella Phage vB_SalP_TR2

et al. 2021

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“…An alternative method for targeting multiple species in a community is with polyvalent phage treatment or phage with host ranges that encompass multiple species. Descriptions of polyvalent phage have increased over the past five years likely due to directed changes in phage isolation protocols (Hamdi et al ., 2017; Duc et al ., 2020; Li et al ., 2020). In fact, Zhao and colleagues used a soil‐carrot microcosm system to compare the efficacy of a cocktail that included phage targeting two different plant pathogens with a treatment of a single polyvalent phage that infected both pathogens (Zhao et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

Phage cocktail strategies for the suppression of a pathogen in a cross‐feeding coculture

Fazzino

Anisman

Chacón

et al. 2020

Microbial Biotechnology

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Predicting which combinations (cocktails) of bacteria‐infecting viruses (bacteriophage) will suppress pathogenic bacterial growth is complicated, in part, due to the complex ecological interactions of host bacteria, but important for effective treatment. We found that when hosts cross‐feed (exchange nutrients) with nonhost bacterial species, cocktails that include a bacteriophage infecting the nonhost cross‐feeding partner can be more effective at suppressing pathogenic bacteria than targeting the pathogenic bacterium with multiple bacteriophage species. This is a novel cocktail formulation.

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“…Escherichia coli (E. coli) O157:H7 is a serotype of enterohemorrhagic Escherichia coli (EHEC) [1][2][3]. As the most harmful food-borne pathogenic bacterium, E. coli O157:H7 can destroy cells and is resistant to gastric acid.…”

Section: Introductionmentioning

confidence: 99%

Microfluidics for the rapid detection of Escherichia coli O157:H7 using antibody-coated microspheres

Shopsin

Sun

et al. 2021

Bioengineered

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This study developed a novel method for the rapid detection of Escherichia coli (E. coli) O157:H7 on a microfluidic platform. First, the concentration of bacteria in a sample was determined with the adenosine triphosphate (ATP) method. Then, the specific detection of E. coli was achieved in a microfluidic chip by the immune-microsphere technique. The influences of the culture time, flow rate and capture time on the detection of the target bacteria were investigated systematically. Generally, with increasing capture time, more bacteria could be captured by the microspheres, which had a positive effect on bacterial detection. Furthermore, the sensitivity and specificity of the method were also tested. The results showed that this method could specifically detect E. coli with a sensitivity as high as 49.1 cfu/μL; the consumption of bacteria was 1 μL, and the reagent was at the microliter level. The testing time can be controlled within one and a half hours, and the cost of testing was approximately RMB 10. The method described in this article is simple and accurate and has great application value in bacterial detection for medical diagnostics.

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Isolation, characterization and application of a polyvalent phage capable of controlling Salmonella and Escherichia coli O157:H7 in different food matrices

Cited by 101 publications

References 97 publications

Isolation and Characterization of a Novel Salmonella Phage vB_SalP_TR2

Isolation and Characterization of a Novel Salmonella Phage vB_SalP_TR2

Phage cocktail strategies for the suppression of a pathogen in a cross‐feeding coculture

Microfluidics for the rapid detection of Escherichia coli O157:H7 using antibody-coated microspheres

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