Isolation, Characterization and Antimicrobial Activity of 3β, 22E-Stigmasta-5, 22-dien-3-ol from the Aerial Part of Aeschynomene uniflora E. Mey

Achika, J.I.; Ndukwe, G. I.; Ayo, Rachael Gbekele-Oluwa

doi:10.9734/bjpr/2016/23506

Cited by 5 publications

(6 citation statements)

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“…is large shrub or small tree up to 9–12 m tall, belonging to the family Fabaceae. It is native to the tropical Africa and Madagascar, cultivated in Egypt, Java and South America [12], Plants of genus Aeschynomene are reported to exert hepatoprotective [13], antimicrobial [14], cytotoxic [15], antioxidant [16], anthelmintic [17] and anti-inflammatory activities [18]. Preliminary phytochemical studies among different Aeschynomene species revealed the presence of flavonoid glycosides [19], pterocarpans [20] and chalcones [21].…”

Section: Introductionmentioning

confidence: 99%

Metabolic profile and hepatoprotective effect of Aeschynomene elaphroxylon (Guill. & Perr.)

Hashem¹,

Salama²,

Mohammed³

et al. 2019

PLoS ONE

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Liver diseases are life-threatening and need urgent medical treatments. Conventional treatment is expensive and toxic, so the urge for nutraceutical hepatoprotective agents is crucial. This study is considered the first metabolic profile of Aeschynomene elaphroxylon (Guill. & Perr.) extracts of; flowers, leaves & bark adopting UPLC-Orbitrap HRMS analysis to determine their bioactive metabolites, and it was designed to investigate the potential hepatoprotective activity of A. elaphroxylon flowers and bark extracts against CCl4-induced hepatic fibrosis in rats. Forty-nine compounds of various classes were detected in the three extracts, with triterpenoid saponins as the major detected metabolite. Flowers and bark extracts presented similar chemical profile while leaves extract was quite different. The antioxidant activities of the flowers, leaves & bark extracts were measured by in vitro assays as Fe+3 reducing antioxidant power and Oxygen radical absorbance capacity. It revealed that flowers and bark extracts had relatively high antioxidant activity as compared to leaves extract. Based on the metabolic profile and in vitro antioxidant activity, flowers and bark ethanolic extracts were chosen for alleviation of hepatotoxicity induced by CCl4 in rats. The hepatoprotective activity was studied through measuring hepatotoxicity biomarkers in serum (ALT, AST, and Albumin). Liver tissues were examined histopathologically and their homogenates were used in determining the intracellular levels of oxidative stress biomarkers (MDA, GSH), inflammatory markers (TNF-α). Flowers and bark ethanolic extracts exerted a significant hepatoprotective effect through reduction in the activities of ALT, AST and Albumin, the tested extracts reduced oxidative stress by increasing GSH content and reducing the MDA level. Furthermore, the extracts decreased levels of pro-inflammatory TNF-α. Moreover, the present study revealed the potentiality of A. elaphroxylon in ameliorating the CCl4-induced hepatic fibrosis in rats. In this aspect, A. elaphroxylon can be used with other agents as a complementary drug.

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Section: Introductionmentioning

confidence: 99%

Metabolic profile and hepatoprotective effect of Aeschynomene elaphroxylon (Guill. & Perr.)

Hashem¹,

Salama²,

Mohammed³

et al. 2019

PLoS ONE

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“…The presence of a pair of doublets at δ 5.01 ppm and δ 5.15 ppm was due to the sp2 methine protons at C22 and C23 in the molecule. The existence of six methyl signals was also noted at δ 0.68 (H3-C18), 1.00 (H3-C19), 0.92 (H3-C21), 0.83 (H3-C26), 0.83 (H3-C27) [13]. Based on the IR, 1 H-NMR, and 13 C-NMR data and in comparison with literatures, suggests the presence of stigmasterol [12,13] .…”

Section: Spectroscopic Datamentioning

confidence: 53%

“…The existence of six methyl signals was also noted at δ 0.68 (H3-C18), 1.00 (H3-C19), 0.92 (H3-C21), 0.83 (H3-C26), 0.83 (H3-C27) [13]. Based on the IR, 1 H-NMR, and 13 C-NMR data and in comparison with literatures, suggests the presence of stigmasterol [12,13] . The Gas Chromatography Mass Spectrometry (Table 4) for compound A1 gave a molecular peak at m/z 412.…”

Section: Spectroscopic Datamentioning

confidence: 53%

“…FT-IR analysis of the samples was obtained and recorded on Perkin Elmer Universal ATR spectrum 100 FT-IR spectrometer. The 1 H-NMR and 13 C-NMR spectra were recorded on 400 MHz Bruker Avance II Ultra shield with samples all dissolved in deuterated chloroform (CDCl3). The GC-MS analysis was carried out on Shimadzu GC-MS-QP-2010-SE.…”

Section: Isolation Of Compounnds From R Adenophyllamentioning

confidence: 99%

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Antioxidant activity and Phytocompounds from Rogeria adenophylla J. Gay crude extract

Mijinyawa¹,

Kwaji²,

Atiko³

et al. 2022

J Pharmacogn Phytochem

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Rogeria adenophylla is a medicinal plant widely used in ethnomedicine for the treatment of inflammations, infectious diseases, wounds and as an antinociceptive. Therefore, there is need to investigate its antioxidant property and identify some of its phytocompounds. The crude extract of R. adenophylla was obtained using maceration technique. The extraction solvent was removed using rotary evaporator at 45 o C to yield a brown residue. The antioxidant activities of fractions were assessed using DPPH while isolation of compounds was achieved on open column chromatography and coded A1 and A3. The characterization of all isolated and purified compounds were carried out using IR, NMR (1D and 2D) and GC-MS data. The study revealed that the ethanol leaf extract possessed the highest antioxidant property of about 87.17% while that of ascorbic acid standard was 75.40%. Compounds A1 and A3 were identified as stigmasterol and palmitic acid methyl ester based on the analysis of all spectroscopic data and in comparison with literature. The presence of these phytocompounds and the observed antioxidant activity justify the use of R.adenophylla in ethnomedicine.

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“…These data have been analyzed, showing the presence of signals at shifts of 140.85 and 121.78 ppm, which are recognized as signals of the C-5 and C-6 double bonds, respectively. The signal that appears at the chemical shift of 71.86 ppm indicates oxygenated carbon, namely carbon bound to hydroxy C-3, which is more deshielded (Achika et al 2016). Another signal appears at the chemical shift of 21.29 ppm and 11.94 ppm, a signal from C-19 and C-18.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Structure Determination of Stigmaterol from the Stem Bark of Lansium domesticum Corr. Cv. Kokossan

2022

CNA

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Indonesia is a tropical country with biodiversity and a source of natural compounds such as terpenoids, phenolic, flavonoids, and alkaloid groups. The steroid is one of the large groups of terpenoid compounds with various structures and bioactivities. The steroid was also commonly found in the Meliaceae family. One species of this family is Lansium domesticum Corr. which has been studied to have various activities from isolated compounds (limonoids, sesquiterpenoids, onoceranoids, cycloartanoids, and steroids) as well as from their extracts such as antidiarrheal, antimalarial, antimicrobial, antiinsecticide, antifeedant and were used as traditional medicine such as eye drops and scorpion stings. The n-hexane fraction from the bark of L. domesticum Cor. cv. Kokosan produced steroid compounds whose structure is determined by IR, 1 H-NMR, 13 C-NMR, and DEPT spectroscopy methods and compared with the literature. It was concluded that the steroid isolated was (22E)-stigmasta-5,22-dien-3ol and was first isolated from this genus. This compound was inactive against MCF-7 breast cancer cells with an IC 50 value of 832.14 µM.

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Isolation, Characterization and Antimicrobial Activity of 3β, 22E-Stigmasta-5, 22-dien-3-ol from the Aerial Part of Aeschynomene uniflora E. Mey

Cited by 5 publications

References 0 publications

Metabolic profile and hepatoprotective effect of Aeschynomene elaphroxylon (Guill. & Perr.)

Metabolic profile and hepatoprotective effect of Aeschynomene elaphroxylon (Guill. & Perr.)

Antioxidant activity and Phytocompounds from Rogeria adenophylla J. Gay crude extract

Isolation and Structure Determination of Stigmaterol from the Stem Bark of Lansium domesticum Corr. Cv. Kokossan

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