Isolation, characterisation, growth and culture of endothelial cells from the rat aorta

Abstract

Rat aortic endothelial cells have been isolated by the explantation technique and grown in culture. They have been identified morphologically using standard staining techniques, biochemically by identification of angiotensin convertase and have been positively stained for Factor VIII-related antigen by immunofluorescence using both anti-human and anti-rat Factor VIII antibodies. The explantation technique is a successful alternative to enzyme digestion which is not applicable to rat aortic endothelial cells because of the nature of their attachment to the subendothelial layer.

Heparin Inhibits the Progression Phase of Subcultured Endothelial Cell Proliferation in Rat Aorta

“…When the growth property of EC was compared with that of SMC from rat aorta, FBS showed greater sensitivity to EC than to SMC in the index of cell number. The pro liferative property of EC with FBS was consistent with the previous report using EC of rat aorta (32). Total 3H -thymidine incorporation into EC , however, was less than half that into SMC.…”

Evidence that [3H]‐α,β‐methylene ATP may label an endothelial‐derived cell line 5′‐nucleotidase with high affinity

“…WEC cells (passages 16 to 25), originally isolated from Wistar rat aortic endothelium (Cole et al, 1986), were grown as a monolayer culture in Dulbecco's modified Eagle's medium containing 10% foetal bovine serum in an humidified atmosphere of 5% CO2 and 95% air at 37°C. For receptor binding assays the WEC cells were grown to 80-90% confluence and then harvested with a cell scraper.…”

“…The WEC cells used in this study are a permanent cell line derived from Wistar rat aortic endothelium (Cole et al, 1986). These cells display many of the characteristics of endothelial cells, including the ability to take up acetylated LDL (T.-P.D.…”

“…As part of a study to screen cell lines for the presence of [3H]-ac,,-meATP binding sites, WEC cells, a rat endothelial cell line (Cole et al, 1986), were found to contain very high levels of [3H]-a,,B-meATP binding. This observation was unexpected since endothelial cells are not thought to contain P2X purinoceptors (Boeynaems & Pearson, 1990).…”

Effects of genistein on angiotensin-converting enzyme in rats

“…The identity of RAECs was confirmed according to the method of Cole et al (1986) with monoclonal anti-rat Factor VIII antibodies. All cells were exclusively factor VIII-positive (Fig.…”