Isolation, Characterisation, and Lipase Production of a Cold-Adapted Bacterial Strain Pseudomonas sp. LSK25 Isolated from Signy Island, Antarctica

Abstract

In recent years, studies on psychrophilic lipases have been an emerging area of research in the field of enzymology. This study focuses on bacterial strains isolated from anthropogenically-influenced soil samples collected around Signy Island Research Station (South Orkney Islands, maritime Antarctic). Limited information on lipase activities from bacteria isolated from Signy station is currently available. The presence of lipase genes was determined using real time quantification PCR (qPCR) in samples obtained from three different locations on Signy Island. Twenty strains from the location with highest lipase gene detection were screened for lipolytic activities at a temperature of 4 °C, and from this one strain was selected for further examination based on the highest enzymatic activities obtained. Analysis of 16S rRNA sequence data of this strain showed the highest level of sequence similarity (98%) to a Pseudomonas sp. strain also isolated from Antarctica. In order to increase lipase production of this psychrophilic strain, optimisation of different parameters of physical and nutritional factors were investigated. Optimal production was obtained at 10 °C and pH 7.0, at 150 rev/min shaking rate over 36 h incubation.

A Broad Temperature Active Lipase Purified From a Psychrotrophic Bacterium of Sikkim Himalaya With Potential Application in Detergent Formulation

“…The growth optima for the organism was also observed in the pH range of 6–10. Similar findings on lipase production in neutral to alkaline media conditions by psychrotrophic bacteria isolated from alpine regions are reported before (Barbaro et al, 2001 ; Gupta et al, 2004 ; Joseph and Ramteke, 2013 ; Salwoom et al, 2019b ). The optimum temperature for enzyme production depends on the growth temperature of the organism.…”

“…Lipases are ubiquitous as they are found in all groups of organisms from bacteria to plants and animals. Although lipases have been studied widely, not much attention has been paid to the bacterial lipases from extreme cold environments (Salwoom et al, 2019b ). Lipases from high altitude bacteria are of significance due to the host of specific structural features that these enzymes have evolved as a mode of adaptation to frequent freezing and thawing conditions.…”

“…Psychrophilic microorganisms are cold-loving with a minimum, optimum, and maximum temperature for growth at about 0, 15, and 20°C, respectively, while psychrotrophic organisms have growth maxima above 25°C but can survive in low temperature (Junge et al, 2019 ). Cold-active enzymes play a major role in their physiological adaptation to low temperature and have been a focus of research at recent times owing to their potential applications in biotechnological industries (Salwoom et al, 2019b ). Irrespective of their significance, cold-active lipases have some limitations like diminished activity at moderate to high temperature, limited substrate specificity, and less thermostability (Joseph et al, 2008 ; Kavitha, 2016 ).…”

Arthrographis curvata and Rhodosporidium babjevae as New Potential Fungal Lipase Producers for Biotechnological Applications

“…This used method of screening for lipaseproducing microorganisms was reported for the first time by Kouker and Jaeger [29]. Since then, this method has been used by Castro-Ochoa and coauthors [30] and many others [31,32,33,34], this procedure has proved to be highly sensitive and reliable.…”

Lipase and esterase activities of lactic acid bacteria isolated from different biotopes

“…Their activators are Zn 2+ , Mg 2+ , Ca 2+ ions as well as NaCl. Phenylmethylsulfonyl fluoride (PMSF), para-chloromercuribenzoate (PCMB), and Hg 2+ , Cu 2+ , Ag 2+ ions inhibit esterase activity (Tsakalidou et al, 1994;Holland and Coolbear, 1996;Fernendez et al, 2000;Nardi et al, 2002;Chich et al, 1997;Fenster et al, 2000;Gobbetti et al, 1997a;Castillo et al, 1999;Choi et al, 2004;Liu et al, 2001;Mobarak-Qamsari et al, 2011;Salwoom et al, 2019).…”