Isolation, characterisation and determination of biological activity of coffee proanthocyanidins

Colmenares, Nélida G. de; Ramirez‐Martinez, J. R.; Aldana, Jose O; Ramos-Nino, M.E.; Clifford, Michael N.; Pékerar, Sara; Méndez, Bernardo

doi:10.1002/(sici)1097-0010(199807)77:3<368::aid-jsfa52>3.0.co;2-v

Cited by 39 publications

(23 citation statements)

References 15 publications

(1 reference statement)

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“…In contrast, previous studies have shown that flavan-3-ols, such as catechin and epicatechin, and oligomeric proanthocyanidins have inhibitory and antifungal properties towards B. cinerea (Goetz et al 1999;Hébert et al 2002) and other fungi (de Colmenares et al 1998;Veluri et al 2004). Proanthocyanidin oligomers specifically inhibit fungal enzymes such as protein kinase and stillbene oxidase (Polya and Foo 1994;Goetz et al 1999).…”

Section: Discussionmentioning

confidence: 87%

Flavonoid biosynthesis and degradation play a role in early defence responses of bilberry (Vaccinium myrtillus) against biotic stress

et al. 2009

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Bilberry (Vaccinium myrtillus) represents one of the richest flavonoid sources among plants. Flavonoids play variable, species-dependent roles in plant defences. In bilberry, flavonoid metabolism is activated in response to solar radiation but not against mechanical injury. In this paper, the defence reaction and biosynthesis of phenolic compounds of bilberry was studied after infection by a fungal endophyte (Paraphaeosphaeria sp.) and a pathogen (Botrytis cinerea). The defence response of bilberry was faster against the endophyte than the pathogen. All flavonoid biosynthesis genes tested were activated by each infection. Biosynthesis and accumulation of phenolic acids, flavan-3-ols and oligomeric proanthocyanidins were clearly elevated in both infected samples. Infection by the pathogen promoted specifically accumulation of epigallocatechin, quercetin-3-glucoside, quercetin-3-O-α-rhamnoside, quercetin-3-O-(4"-HMG)-R-rhamnoside, chlorogenic acid and coumaroyl quinic acid. The endophyte-infected plants had a higher content of quercetin-3-glucuronide and coumaroyl iridoid. Therefore, accumulation of individual phenolic compounds could be specific for each infection. Quantity of insoluble proanthocyanidins was the highest in control plants, suggesting that they might act as storage compounds and become activated by degradation upon infection.

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Section: Discussionmentioning

confidence: 87%

Flavonoid biosynthesis and degradation play a role in early defence responses of bilberry (Vaccinium myrtillus) against biotic stress

et al. 2009

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“…Similar results have been shown in other woody plant species. For example, crude extract from coffee (Coffea arabica) cultivars resistant to coffee rust (Hemileia vastatrix), which contained higher amounts of PAs in comparison with the extracts of susceptible cultivars, was found more effective in inhibiting H. vastatrix uredospore germination (de Colmenares et al, 1998). In addition, higher levels of constitutive and induced (+)-catechin were found in a rust-resistant willow (Salix myrsinifolia) clone compared with the levels in susceptible clones (Hakulinen et al, 1999).…”

Section: High Levels Of Catechin and Pas Are Associated With Resistanmentioning

confidence: 99%

Flavan-3-ols Are an Effective Chemical Defense against Rust Infection

et al. 2017

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Phenolic secondary metabolites are often thought to protect plants against attack by microbes, but their role in defense against pathogen infection in woody plants has not been investigated comprehensively. We studied the biosynthesis, occurrence, and antifungal activity of flavan-3-ols in black poplar (Populus nigra), which include both monomers, such as catechin, and oligomers, known as proanthocyanidins (PAs). We identified and biochemically characterized three leucoanthocyanidin reductases and two anthocyanidin reductases from P. nigra involved in catalyzing the last steps of flavan-3-ol biosynthesis, leading to the formation of catechin [2,3-trans-(+)-flavan-3-ol] and epicatechin [2,3-cis-(2)-flavan-3-ol], respectively. Poplar trees that were inoculated with the biotrophic rust fungus (Melampsora larici-populina) accumulated higher amounts of catechin and PAs than uninfected trees. The de novo-synthesized catechin and PAs in the rust-infected poplar leaves accumulated significantly at the site of fungal infection in the lower epidermis. In planta concentrations of these compounds strongly inhibited rust spore germination and reduced hyphal growth. Poplar genotypes with constitutively higher levels of catechin and PAs as well as hybrid aspen (Populus tremula 3 Populus alba) overexpressing the MYB134 transcription factor were more resistant to rust infection. Silencing PnMYB134, on the other hand, decreased flavan-3-ol biosynthesis and increased susceptibility to rust infection. Taken together, our data indicate that catechin and PAs are effective antifungal defenses in poplar against foliar rust infection.

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“…The major function of proanthocyanidins in plants is to provide protection against microbial pathogens, insect pests and larger herbivores. Their deposition in the endothelial layer of the seed coat appear to be an example of a pre-formed protective barrier 104 . Salmonella, Staphylococcus, Helicobacter and Bacillus are the most sensitive bacteria to the berry phenolics.…”

Section: Proanthocyanidinsmentioning

confidence: 99%

Berry Fruits as a Source of Biologically Active Compounds: The Case of Lonicera Caerulea

Švarcová

Heinrich²,

Valentová³

2007

BIOMED PAP

102

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Isolation, characterisation and determination of biological activity of coffee proanthocyanidins

Cited by 39 publications

References 15 publications

Flavonoid biosynthesis and degradation play a role in early defence responses of bilberry (Vaccinium myrtillus) against biotic stress

Flavonoid biosynthesis and degradation play a role in early defence responses of bilberry (Vaccinium myrtillus) against biotic stress

Flavan-3-ols Are an Effective Chemical Defense against Rust Infection

Berry Fruits as a Source of Biologically Active Compounds: The Case of Lonicera Caerulea

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