Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran

Jannah, Akyunul; Aulanni’am, Aulanni’am; Ardyati, Tri; Suharjono, Suharjono

doi:10.22146/ijc.26783

Cited by 8 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stationary growth phase of cellulolytic bacteria occurred from 24 h until 64 h for A1D, A1I, A2A, and B1A, then followed by the death phase at 72 h, whereas A1E and A1K still in the stationary growth phase at 72 h. In the stationary growth phase, there is a balance between the number of live cells and dead cells [23]. Each bacterial isolate with a density of 10 7 cells.mL -1 at exponential growth phase (24 h incubation) had the highest potency for the production of cellulase enzymes A similar study showed the exponential growth phase of cellulolytic bacteria occurs at 4 -24 h incubation [13]. Another research was reported that the production of cellulase enzymes at 24 h incubation [24].…”

Section: Growth Curve Of Selected Cellulolytic Bacteriamentioning

confidence: 83%

“…The crude enzyme was obtained by centrifugation of culture medium at 4°C, 10.000 rpm for 10 minutes. The supernatant was defined as a crude cellulase enzyme [13].…”

Section: Growth Curve and Crude Enzyme Production Of Selected Bacteriamentioning

confidence: 99%

“…One unit of cellulase enzyme activity is defined as the amount of enzyme that releases 1 μmol glucose per mL per minute. The values of the cellulolytic activity were determined based on the glucose standard curve [13].…”

Section: Cellulolytic Activity Assaymentioning

confidence: 99%

“…2), whole isolates were not showed an adaptation phase. The cell density of those isolates was increased significantly at the logarithmic/exponential growth phase from initial incubation until 24 h. In the exponential growth phase, bacteria perform constantly at a maximum growth rate of cell division [13]. The bacterial cells had an optimal production of cellulase at the exponential growth phase [21].…”

Section: Growth Curve Of Selected Cellulolytic Bacteriamentioning

confidence: 99%

See 3 more Smart Citations

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Faizah

Ardyati

Suharjono

2020

JELS

Self Cite

View full text Add to dashboard Cite

Palopo, South Sulawesi, is one of the traditional industrial centers of sago processing. The accumulation of sago pith waste around industrial sites can pollute the environment. Some microorganisms can degrade the cellulose in sago pith waste. This study was aimed to evaluate the indigenous cellulolytic bacteria from sago pith waste as a biodegradation agent. Bacteria were isolated from sago pith waste and grown on a 1% Carboxyl methyl cellulose (CMC) agar medium. The cellulolytic activity was analyzed semiquantitatively using 1% Congo red and quantitatively using the 3,5-Dinitrosalicylic Acid (DNS) method at pH variations of 4, 5, and 6. The potential isolate was identified based on 16S rDNA sequence similarity. This study obtained 21 bacterial isolates where six isolates were A1D, A1E, A1I, A1K, A2A, and B1A had the highest cellulolytic index at 0.82-1.13. Among those six isolates, the A1E isolate had the highest cellulolytic activity, 0.54 U.mL-1 at pH 6. The isolate A1E was identified as Burkholderia cepacia JCM 2799 with 99.73% similarity of 16S rDNA sequence.

show abstract

Section: Growth Curve Of Selected Cellulolytic Bacteriamentioning

confidence: 83%

“…The crude enzyme was obtained by centrifugation of culture medium at 4°C, 10.000 rpm for 10 minutes. The supernatant was defined as a crude cellulase enzyme [13].…”

Section: Growth Curve and Crude Enzyme Production Of Selected Bacteriamentioning

confidence: 99%

Section: Cellulolytic Activity Assaymentioning

confidence: 99%

Section: Growth Curve Of Selected Cellulolytic Bacteriamentioning

confidence: 99%

See 2 more Smart Citations

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Faizah

Ardyati

Suharjono

2020

JELS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The solution was cooled for 20 min and the absorbance was measured using a spectrophotometer at 540 nm. One activity unit (U/mL) is expressed as the total amount of reduced sugars (1000 μmoL) in the reaction mixture per 30 min [11,12].…”

Section: Cellulase Assaymentioning

confidence: 99%

Potential of cellulolytic fungi isolated from the intestine of Asiatic rhinoceros beetle (Oryctes rhinoceros)

Nasution

Munir

Suryanto

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Cellulose is a potential source of fermentable sugars and a promising cost-effective source for biofuel production. This study aimed to obtain potential cellulolytic fungi from the intestine of Asiatic rhinoceros beetle (Oryctes rhinoceros L.). The cellulolytic fungi were isolated using Potato Dextrose Agar (PDA) media using spread plate method. Qualitative and quantitative screening tests were carried out using 1% of CMC (w/v) as a sole carbon source. Twelve isolates were recovered with varying cellulolytic capabilities. The highest cellulolytic activity was obtained from KOr-15J with an index of 1.93 while KOr-23J produced the lowest cellulolytic index (1.01) based on the qualitative screening. The highest cellulase activity assayed using DNS (3,5-Dinitrosalicylic acid) method revealed KOr-21J with an activity of 0.018 U/mL while three isolates (KOr-16J, KOr-19J and KOr-22J) produced the lowest activity (0.003 U/mL). This study provides multiple verifications of the potential ability of cellulolytic fungi and their cellulase activity in the intestine of Asiatic rhinoceros beetle.

show abstract

Co-bioaugmentation with microalgae and probiotic bacteria: Sustainable solutions for upcycling of aquaculture wastewater and agricultural residues into microbial-rice bran complexes

Pekkoh,

Thurakit,

Ruangrit

et al. 2024

Environmental Research

View full text Add to dashboard Cite

Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran

Abstract: Rice bran is the waste product of rice milling which is abundant in

Cited by 8 publications

References 20 publications

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Isolation and Identification of Indigenous Cellulolytic Bacteria from Sago Pith Waste at Palopo, South Sulawesi, Indonesia

Potential of cellulolytic fungi isolated from the intestine of Asiatic rhinoceros beetle (Oryctes rhinoceros)

Co-bioaugmentation with microalgae and probiotic bacteria: Sustainable solutions for upcycling of aquaculture wastewater and agricultural residues into microbial-rice bran complexes

Contact Info

Product

Resources

About