Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: correlation with real-time reverse transcriptase–polymerase chain reaction results and feasibility of molecular analysis by laser microdissection

Pinzani, Pamela; Salvadori, Barbara; Simi, Lisa; Bianchi, Simonetta; Distante, V.; Cataliotti, Luigi; Pazzagli, Mario; Orlando, Claudio

doi:10.1016/j.humpath.2006.01.026

Cited by 148 publications

(96 citation statements)

References 17 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This methodology allows "classical" cytopathologic criteria of malignancy, already used in exfoliative cytology, to be used to identify malignant tumor cells (14). Currently, ISET technology has been reported previously to allow identification of CTC in patients with liver or breast cancers (15,16). However, the ISET method has never been used to detect CTC in patients with NSCLC.…”

Section: Introductionmentioning

confidence: 99%

Preoperative Circulating Tumor Cell Detection Using the Isolation by Size of Epithelial Tumor Cell Method for Patients with Lung Cancer Is a New Prognostic Biomarker

Hofman

Bonnetaud

Ilié

et al. 2011

Clinical Cancer Research

278

269

View full text Add to dashboard Cite

Purpose: Pathologic TNM staging is currently the best prognostic factor for non-small cell lung carcinoma (NSCLC). However, even in early-stage NSCLC, the recurrence rates after surgery range from 25% to 50%. The preoperative detection of circulating tumor cells (CTC) could be useful to tailor new therapeutic strategies in NSCLC. We assessed the presence of CTC in NSCLC patients undergoing surgery, using cytologic analyses, after their isolation by size of epithelial tumor cells (ISET method). The presence and the number of CTCs were considered and correlated with clinicopathologic parameters including patient follow-up.Experimental design:Of the 247 blood samples tested, 208 samples were from patients with resectable NSCLC and 39 from healthy subjects. The mean follow-up was 24 months. An image of detected cells with presumably nonhematologic features [initially defined as "circulating nonhematologic cells" (CNHC)] was recorded. The presence of CNHC was assessed blindly and independently by 10 cytopathologists, using cytologic criteria of malignancy on stained filters. The count of detected CNHCs was made for each filter.Results: One hundred two of 208 (49%) patients showed CNHCs corresponding to CNHC with malignant cytopathologic features in 76 of 208 (36%) cases. CNHCs were not detected in the control group. A level of 50 or more CNHCs corresponding to the third quartile was associated with shorter overall and disease-free-survival, independently of disease staging, and with a high risk of recurrence and death in early-stage I þ II-resectable NSCLC.Conclusion: A high percentage of NSCLC patients show preoperative detection of CNHC by the ISET method. The presence and level of 50 or more CNHCs are associated with worse survival of patients with resectable NSCLC.

show abstract

Section: Introductionmentioning

confidence: 99%

Preoperative Circulating Tumor Cell Detection Using the Isolation by Size of Epithelial Tumor Cell Method for Patients with Lung Cancer Is a New Prognostic Biomarker

Hofman

Bonnetaud

Ilié

et al. 2011

Clinical Cancer Research

278

269

View full text Add to dashboard Cite

show abstract

“…A comparison of ISET technology for CTC detection and real-time PCR resulted in a statistically significant agreement of the two methods in their detection frequency (154), but a substantial variation in detection rates was seen comparing real-time PCR with the CellSearch ® system and the AdnaTest, in which real-time PCR with CK19 and hMAM seemed to be the most sensitive of the three methods investigated (76).…”

Section: Recent Developments For Ctc Detection In Breast Cancermentioning

confidence: 99%

Real-time RT-PCR systems for CTC detection from blood samples of breast cancer and gynaecological tumour patients (Review)

et al. 2016

View full text Add to dashboard Cite

“…Size based separation protocols utilize the property of tumour cells being at least twice the size of the majority of leukocytes (>20µm versus 8 -10µm). Blood is passed through filters which, with the help of a gentle vacuum pressure, exclude CTCs and a small number of larger white blood cells such as macrophages and monocytes (Pinzani et al, 2006). One obvious advantage of this type of separation is that CTCs are not being selected for by epithelial cell surface markers which might be lost during the aforementioned EMT processes.…”

Section: Physical Properties Based Enrichmentmentioning

confidence: 99%

Circulating Tumour Cells: Implications and Methods of Detection

Kanwar¹,

Done²

2011

Breast Cancer - Recent Advances in Biology, Imaging and Therapeutics

View full text Add to dashboard Cite

Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: correlation with real-time reverse transcriptase–polymerase chain reaction results and feasibility of molecular analysis by laser microdissection

Cited by 148 publications

References 17 publications

Preoperative Circulating Tumor Cell Detection Using the Isolation by Size of Epithelial Tumor Cell Method for Patients with Lung Cancer Is a New Prognostic Biomarker

Preoperative Circulating Tumor Cell Detection Using the Isolation by Size of Epithelial Tumor Cell Method for Patients with Lung Cancer Is a New Prognostic Biomarker

Real-time RT-PCR systems for CTC detection from blood samples of breast cancer and gynaecological tumour patients (Review)

Circulating Tumour Cells: Implications and Methods of Detection

Contact Info

Product

Resources

About