Isolation, biochemical characterization and anti-bacterial activity of BPIFA2 protein

Prokopović, Vladimir Z.; Popović, Milica; Andjelković, Uroš; Maršavelski, Aleksandra; Rašković, Božidar; Gavrović-Jankulović, Marija; Polović, Natalija

doi:10.1016/j.archoralbio.2013.12.005

Cited by 22 publications

(19 citation statements)

References 23 publications

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“…Results were expressed as the percentage of yeast growth compared to the control sample incubated in the appropriate buffer in the absence of latex (100 mmol L −1 sodium phosphate buffer, pH 7.4, added to yeast culture). The mean growth values of triplicates were obtained and then converted to the percentage inhibition of cell growth, as described by Prokopovic et al …”

Section: Methodsmentioning

confidence: 99%

Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening

2015

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Section: Methodsmentioning

confidence: 99%

Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening

2015

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“…8 Therefore, studies of salivary proteins and peptides indicate that these substances have diagnostic and interventional potential in several clinical situations, 4 which may allow the development of prevention programs or individualized treatment. 9 Given the role of salivary proteins in caries pathophysiology, their related genes may also be strong candidates for explaining genetic variation in caries experience in human populations. However, the boom of genetic association studies in recent decades has given rise to controversy and conflicting or irreproducible results, mainly due to differences in study design, statistical analysis, and interpretation of findings.…”

Section: Declaration Of Interestsmentioning

confidence: 99%

Salivary protein polymorphisms and risk of dental caries: a systematic review

et al. 2017

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Dental caries is an oral pathology associated with both lifestyle and genetic factors. The caries process can be influenced by salivary composition, which includes ions and proteins. Studies have described associations between salivary protein polymorphisms and dental caries experience, while others have shown no association with salivary proteins genetic variability. The aim of this study is to assess the influence of salivary protein polymorphisms on the risk of dental caries by means of a systematic review of the current literature. An electronic search was performed in PubMed, Scopus, and Virtual Health Library. The following search terms were used: "dental caries susceptibility," "dental caries," "polymorphism, genetics," "saliva," "proteins," and "peptides." Related MeSH headings and free terms were included. The inclusion criteria comprised clinical investigations of subjects with and without caries. After application of these eligibility criteria, the selected articles were qualified by assessing their methodological quality. Initially, 338 articles were identified from the electronic databases after exclusion of duplicates. Exclusion criteria eliminated 322 articles, and 16 remained for evaluation. Eleven articles found a consistent association between salivary protein polymorphisms and risk of dental caries, for proteins related to antimicrobial activity (beta defensin 1 and lysozyme-like protein), pH control (carbonic anhydrase VI), and bacterial colonization/adhesion (lactotransferrin, mucin, and proline-rich protein Db). This systematic review demonstrated an association between genetic polymorphisms and risk of dental caries for most of the salivary proteins.

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“…Serinc3 was also up-regulated in nicotine-treated cells in various cell lines including, pancreatic stellate cells [6] , HEK293 [22] , HeLa [22] , SH-SY5Y [22] , HPNE [23] and Panc1 [23] cells. Bpifa2 (BPI fold A2) lipopolysaccharide binding protein participates in lipid transport [24] , and shows a significant increase in abundance in five tissues: lung, spleen, kidney, liver, and heart. Cyp2b10 (cytochrome P450 2B10) is a liver enzyme that oxidases a variety of compounds including steroids and fatty acids [25] .…”

Section: Resultsmentioning

confidence: 99%

Multiplexed Isobaric Tag‐Based Profiling of Seven Murine Tissues Following In Vivo Nicotine Treatment Using a Minimalistic Proteomics Strategy

et al. 2018

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Nicotine is a major addictive compound in tobacco and a component of smoking-related products, such as e-cigarettes. Once internalized, nicotine can perturb many cellular pathways and can induce alterations in proteins across different cell types, however the mechanisms thereof remain undetermined. We hypothesize that both tissue-specific and global protein abundance alterations result from nicotine exposure. As such, we present the first proteome analysis of multiple tissues from nicotine-exposed mice. We treat mice via oral administration of nicotine at 200μg/mL in drinking water for 21 days. We investigate 7 tissues (brain, heart, kidney, liver, lung, pancreas, and spleen) from treated (n=5) and untreated control (n=5) mice. For each tissue, a TMT10-plex experiment (5 versus 5) was assembled. We apply a minimalistic proteomics strategy was employed using TMT reagents efficiently and fractionating by centrifugation-based reversed-phase columns to streamline sample preparation. Combined, we quantified over 11,000 non-redundant proteins from over 138,000 different peptides in 7 TMT10-plex experiments. Between 7 and 126 proteins are significantly altered in tissues from nicotine-exposed mice. Among these proteins, only 11 are altered in two or more tissues, many classified as from extracellular exosomes or involved in lipid metabolism. Our data showcase the vast extent of nicotine exposure across murine tissue.

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Isolation, biochemical characterization and anti-bacterial activity of BPIFA2 protein

Cited by 22 publications

References 23 publications

Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening

Characterisation of general proteolytic, milk clotting and antifungal activity of Ficus carica latex during fruit ripening

Salivary protein polymorphisms and risk of dental caries: a systematic review

Multiplexed Isobaric Tag‐Based Profiling of Seven Murine Tissues Following In Vivo Nicotine Treatment Using a Minimalistic Proteomics Strategy

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