Isolation and Study on Pure Culture of Wild Edible Mushrooms Collected From Provinces in the Southeast Region of Viet Nam

Abstract

The present study aimed to characterize, classify and attempt to purely culture wild edible mushrooms collected from several provinces in the Southeast region of Viet Nam. We isolated seven mushroom strains that belonged to different genera, Phlebia sp., Ganoderma sp., Tricholoma sp., Polyporus sp., Panus sp., Pleurotus sp. and Phlebopus sp. These mushroom mycelia were able to well grow on both of PGA and modified PGA media. Most of the strains, except for Phlebopus sp (M3), were able to grow on unhusked rice medium supplemented with rice bran and corn bran. Among them, Ganoderma sp. (M20) exhibited the highest mycelial growth rate (7.8 mm/day). The other strains had growth rates of 4.0 -5.2 mm/day. Rubber tree sawdust supplemented with corn bran or earthworm fertilizer was used for production of fruiting bodies. The results indicated that Phlebia sp. (M10) gave better yields of fruiting bodies (536-539 g/kg dw on the media supplemented with 10 or 15 % corn bran or 5 % earthworm fertilizer) than those of Ganoderma sp. and Pleurotus sp. (M4), 164 and 153 g/kg dw, respectively, on the medium with 5 % corn bran. The mycelia from rice grain spawn of the remaining other strains failed to colonize or form fruiting bodies on the tested substrates.