Isolation and study of promising strains of phosphate-mobilizing microorganisms for the creation of biofertilizer

Abstract

The article describes the process of isolation and study of strains of phosphate-mobilizing microorganisms. As a result of soil analysis, the selected 9 samples contain a sufficient amount of total nitrogen, humus and mobile phosphorus compounds for the development of microorganisms. As a result of the isolation of phosphate-mobilizing microorganisms, 23 mono-isolates were selected. After incubation of the flasks, analyzes for the content of dissolved phosphorus and pH in the culture media were carried out. As a result, the highest content of dissolved phosphorus was obtained by incubation of monoisolates No. 9 and 20, which were 3.57 g/l and 2.55 g/l, respectively. Also, the pH of the medium in these monoisolates showed the lowest values: 5.4 and 5.7, respectively. Monoisolates No. 9 and 20 are named FT1 and FT2, respectively. Based on physiological and biochemical studies, isolated monoisolates were identified as Bacillus megaterium FT1 and Bacillus sp. FT2. As a result of studying phosphate-mobilizing activity in the isolated strains of soil phosphate mobilizing microorganisms and the collection strain, the new strain of B. megaterium FT1 showed the highest phosphate mobilizing activity, where 100 % of phosphorus in the medium were dissolved within 120 hours. The yield of dissolved phosphorus in the medium with the strains of Bacillus spp. FT2 and Serratia plymuthica Nl1 were 50.0 5 and 54.2 %, respectively, for 168 hours.