Isolation and study of microorganisms from oil samples for application in Microbial Enhanced Oil Recovery

Abstract

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Enzyme-mediated biodegradation of long-chain n-alkanes (C32 and C40) by thermophilic bacteria

“…In BH broth, isolates IR2, IR4 and MN6 were proved to be potentially adhering to the n-alkanes surface and utilized as carbon sources to form a new biomass (Rojo 2010). Ibrahim et al (2013) and Gudina et al (2012) reported that the B. licheniformis were dominant bacterial strain in oil reservoir and capable to degrade several mercaptans such as 1-decanethiol and 1-dodecanethiol at 55°C (Chebbi et al 2014). In this study, the MN6 was able to perform superior than other strains or mixed consortium in degradation of C 40 .…”

Isolation and screening of Bacillus subtilis MJ01 for MEOR application: biosurfactant characterization, production optimization and wetting effect on carbonate surfaces

“…After 1-3-day incubation at 30 °C, morphologically different colonies were re-cultivated in fresh agar plates at least for three times to obtain pure cultures. Purity of isolates was confirmed through Gram staining (Gudiña et al 2012).…”

Effects of the addition of waste cooking oil on heavy crude oil biodegradation and microbial enhanced oil recovery using Pseudomonas sp. SWP- 4

“…One of the primary mechanisms of heavy crude oil viscosity reduction is that degradation of heavy oil fractions through microbial activities, and the other one is that the produced rhamnolipid could enhance the stability of oil/water emulsion system, eventually forming oil-in-water [2,4,24]. The high contents of the employed heavy oil fractions resulted in its high viscosity and poor fluidity.…”

“…MEOR makes use of microbial activities and metabolic byproducts to reduce the viscosity and increase the fluidity of crude oil [4]. On the one hand, microorganisms can reduce the oil viscosity by degrading the long-chain saturated hydrocarbons and some of the other heavy oil fractions [5]; On the other hand, microorganisms can produce non-toxic products such as biosurfactants to increase the oil sweep efficiency through changing the reservoirs' physicochemical characteristics [6].…”