Isolation and study of functional mast cells from lung and mesentery of the guinea pig

Pearce, F. L.; Behrendt, Heidrun; Blum, Ulrike; Poblete-Freundt, G.; Pult, P.; Stang-Voss, Ch.; Schmutzler, W.

doi:10.1007/bf01964880

Cited by 39 publications

(12 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These differences are in agreement with functional studies showing that the mast cell populations iso lated from the guinea pig and rat lung have different properties [10,11,[22][23][24].…”

Section: Discussionsupporting

confidence: 81%

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Bachelet¹,

Bernaudin²,

Fleury‐Feith³

1988

Int Arch Allergy Immunol

View full text Add to dashboard Cite

The rat and guinea pig are widely used in experimental models dealing with immuno allergic bronchoreactivity. The present study was designed to compare the distribution and heterogeneity of the mast cell population in the respiratory tract of the Sprague-Dawley rat and Hartley guinea pig. Mast cells were identified according to the sequential Alcian blue/safranin·staining method. From the trachea to the peripheral conductive airways, the density of mast cells increased in the guinea pig whereas it decreased in the rat. Although mast cells were observed in the interalveolar septa of the guinea pig, none were found in the rat. In the lung of the rat, three types of mast cells were observed: in the wall of the trachea and large bronchi, safranin-positive, mixed and Alcian-blue-positive mast cells were found, whereas only Alcian-blue-positive mast cells were observed in the small conductive airways. In contrast, only Alcian-blue-positive mast cells were observed in the lung of the guinea pig. These results show that the types and localization of the mast cell populations are distinctly different in these two species. Consequently, experimental models of bronchoreactivity in these species are testing different cellular systems.

show abstract

“…These differences are in agreement with functional studies showing that the mast cell populations iso lated from the guinea pig and rat lung have different properties [10,11,[22][23][24].…”

Section: Discussionsupporting

confidence: 81%

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Bachelet¹,

Bernaudin²,

Fleury‐Feith³

1988

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…Numerous recent studies deal with the mea surement of released mediators, but some of these products are not representative of mast cell activity only and, moreover, in vitro studies with passively sensitized tissues which are usually performed are far from representative of physiological conditions [12,13]. The present study revealed the mast cell reactivity in vivo, during sensitization and challenge.…”

Section: Discussionmentioning

confidence: 52%

Histological Study of Mast Cells in the Actively Sensitized Guinea Pig Lung and after Challenge: Effect of a Corticosteroid

Bachelet¹,

Bernaudin²,

Fleury‐Feith³

1990

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Although mast cells are sometimes considered to play a minor role in hypersensitivity reactions, we used a histological method to show the modifications in the guinea pig lung mast cell population in the course of such reactions. We sensitized guinea pigs with ovalbumin and studied the effect of the challenge with and without corticosteroid treatment. We observed that the mast cell count was not modified after sensitization but was decreased after challenge. Twenty-four hours after challenge, the number of mast cells returned to the control value, indicating a renewal of the mast cell pool. A second challenge, 1 week after the first, did not provoke the same mast cell degranulation, suggesting a non-responsiveness to aerosol antigen. Betamethasone dipropionate treatment protected mast cells against challenge: in treated guinea pigs, mast cell degranulation was prevented, and we did not observe any change in mast cell count after challenge. The present study was useful to show an effect of corticosteroids on mast cell degranulation in immediate hypersensitivity reactions in vivo.

show abstract

“…(9). The role of mast cells on the 8-day PCA in guinea pigs has also been considered to be the same as that on the IgE-mediated PCA in rats (24). All these findings taken together suggest that the mode of the inhibitory action of AA-344 on the 8-day PCA in guinea pigs was the same as that on the 72-hr PCA in rats.…”

Section: Discussionmentioning

confidence: 86%

Effect of 6-ethyl-3-(1H-tetrazol-5-yl)chromone (AA-344) on the immediate and delayed hypersensitivity reactions.

et al. 1979

View full text Add to dashboard Cite

Abstract-Effects of 6-ethyl-3-(1 H-tetrazol-5-yl)chromone (AA-344) on the experimental models of the type I-I V allergic reactions were studied in comparison with those of disodium cromoglycate (DSCG), dexamethasone and other agents. AA-344 showed inhibitory effects on the homologous passive cutaneous anaphylaxis and the passive systemic anaphylaxis in guinea pigs. However, it had only a slight or little effect on the Forssman shock in guinea pigs, the complement-dependent cytolysis of mast cells in rats, the Arthus reaction in guinea pigs and the tuberculin reaction and the contact sensitivity in mice. DSCG was less effective, using these experimental models. Dexamethasone showed a suppressive effect on the type III and IV allergic reactions. The results indicate that AA-344 selectively suppresses the type I allergic reaction.We previously reported that 6-ethyl-3-(I H-tetrazol-5-yl)chromone (AA-344) by either i.v. or p.o. administration markedly suppressed the IgE-mediated hypersensitivity due to inhibition of the release of chemical mediators from the mast cells (1, 2). The mode of action of AA-344 in vivo and in vitro on the type I allergic reaction, is similar to that of disodium cromoglycate (DSCG) (3, 4).The inhibitory effect of DSCG on the late asthmatic reaction provoked by inhalation of antigen in patients with asthma, which is classified as the type III allergic reaction (3, 5), was reported by some groups (5, 6), while such could not be confirmed by another group (7).There is apparently no documentation concerning the effect of DSCG on the experimental model of type III allergic reaction in animals. The present study was an attempt to determine whether AA-344 or DSCG, an inhibitor of chemical mediator release from mast cells, has a suppressive effect not only on type I allergic reaction but also on the type II, III and IV allergic reactions, as compared with effects of dexamethasone and antihistamines, in experimental models. MATERIALS AND METHODSAnimals: The animals used were male JCL:ICR mice (25-31 g), male C57BL/6 mice (21-34 g), male BALB/c mice (19-27 g), male Sprague-Dawley rats (250-350 g), Hartley guinea pigs of either sex (280-450 g) and male albino rabbits (2.5-4.0 kg).Chemicals: The following chemicals were used: bovine-r-globulin (BGG, Wako), bovine serum albumin fraction V (BSA, Wako), egg albumin (EA, Difco), Freund's complete

show abstract

Isolation and study of functional mast cells from lung and mesentery of the guinea pig

Cited by 39 publications

References 17 publications

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Distribution and Histochemical Characterization of Pulmonary Mast Cells in the Rat and Guinea Pig

Histological Study of Mast Cells in the Actively Sensitized Guinea Pig Lung and after Challenge: Effect of a Corticosteroid

Effect of 6-ethyl-3-(1H-tetrazol-5-yl)chromone (AA-344) on the immediate and delayed hypersensitivity reactions.

Contact Info

Product

Resources

About