Isolation and study of a strain of lumpy skin disease virus of cattle

Abstract

The identification of new virus strains will prevent the development of outbreaks thanks to the development and use of vaccines. The aim of the work was to isolate and sequence the genome of lumpy skin disease virus from an epizootic in the Kostanay region. The genetic material of virions was identified by polymerase chain reaction (PCR); viral antigens and antibodies to them have been determined by enzyme-linked immunosorbent assay (ELISA) or diffusion test methods; to accumulate the material, the virus has been cultivated in lamb testicle monoclonal cell; new generation sequencing has been performed using MiSeq System and FastQ software; strain affiliation has been established by the BLASTN-alignment method. Specific amplicons of the virus with a length of 347 bp. were detected in skin samples but not found in blood. Antigens in 1:5-1:320 dilutions were identified in skin material and 2-3 passages of monoclonals; less antigenic activity was found in the blood in a 1:2 dilution. In response to the pathogen, specific immunoglobulins were synthesized in the serum of 67% of the studied animals and were detected in dilutions of 1:100-1:400. The viral material was accumulated in monoclonals and isolated in a sucrose gradient. The whole-genome sequence of the obtained material confirmed the isolation of a new strain of nodular dermatitis virus with a percentage of similarity to the closest homologues of 99.66%. The strain was named Dermatitis nodularis bovum/2018/Kostanay/KZ; the sequence has been submitted to GeneBank, and the object has been deposited in the Collection of Microorganisms under accession number M-9-21/D. The obtained information can be used to prevent the spread of foci of cattle infection