Isolation and structure of red pigments from Aspergillus flavus and related species, grown on a differential medium

Assante, Gemma; Camarda, Lawrence; Locci, R.; Merlini, L.; Nasini, Gianluca; Papadopoulos, Elisabetta

doi:10.1021/jf00106a023

Cited by 44 publications

(34 citation statements)

References 4 publications

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“…Assante et al [1981] suggested that ferric ions in medium react with aspergillic acid or neoaspergillic acid produced by Aspergillus species and form a yelloworange colored complex. In the present study, 41 of 51 A. oryzae isolates from Nuruk samples showed strong positive reactions.…”

Section: Resultsmentioning

confidence: 99%

Fungi associated with the traditional starter cultures used for rice wine in Korea

Yang

Lee

Kwak

et al. 2011

J Korean Soc Appl Biol Chem

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The 'Nuruk' starter culture has been used for many years in the fermentation of rice wine in Korea. In present study, mycobiota in Nuruk cultures was identified by performing morphological, physiological, and phylogenetical analyses. Mucorales was the most common mycobiota in Nuruk, followed by yeast and Aspergillus. The composition of fungal species in Nuruk was different among samples and did not correlate with the geographical location from where the Nuruk culture was manufactured. For more detailed identification, 174 filamentous fungal strains were isolated from 39 Nuruk samples. Although the morphological and molecular analyses showed that the strains were identical at the genus level, some discordance was identified between species. Of the 174 strains, 160 showed thermotolerance, and the level of thermotolerance matched the clade generated by phylogenetic analysis. Six genera (Lichtheimia, Aspergillus, Rhizopus, Rhizomucor, Mucor, and Syncephalastrum) and 17 fungal species were identified. Among the genera, the genus Syncephalastrum had not been previously identified in Nuruk cultures, and the isolate was identified as Syncephalastrum racemosum. Two genera, Lichtheimia and Aspergillus, comprised approximately 84% of the filamentous fungal isolates from the Nuruk samples, and Lichtheimia ramosa and Aspergillus oryzae were the most commonly found species. The controversy regarding the presence of mycobiota in Nuruk starter cultures was addressed, and results showed that Nuruk contains unique mycobiota not yet found in other Asian starter cultures.

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Section: Resultsmentioning

confidence: 99%

Fungi associated with the traditional starter cultures used for rice wine in Korea

Yang

Lee

Kwak

et al. 2011

J Korean Soc Appl Biol Chem

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“…It was just recently that the biosynthetic pathway of ditryptophenaline was fully elucidated, showing it to be accomplished by a NRPS gene cluster (Springer et al 1977;Saruwatari et al 2014). Also other previously described metabolites with similar structural patterns such as aspergillic or neoaspergillic acid are probably synthesised in the same fashion by employing non-ribosomal peptide synthetases (Assante et al 1981). On the other hand, there are scarce data demonstrating the ability of A. flavus to produce hybrid polyketide-aminoacid molecules.…”

Section: Introductionmentioning

confidence: 90%

Use of UHPLC high-resolution Orbitrap mass spectrometry to investigate the genes involved in the production of secondary metabolites inAspergillus flavus

Arroyo‐Manzanares

Mavungu

Uka

et al. 2015

Food Additives & Contaminants: Part A

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The fungus Aspergillus flavus is known for its ability to produce the toxic and carcinogenic aflatoxins in food and feed. While aflatoxins are of most concern, A. flavus is predicted to be capable of producing many more metabolites based on a study of its complete genome sequence. Some of these metabolites could be of great importance in food and feed safety. Here we describe an analytical methodology based on Orbitrap HRMS technology that allows the untargeted determination of fungal metabolites, in support of the study of the function of genes involved in secondary metabolism in fungi. The applied strategy implies the detection and identification of differentially expressed metabolites in extracts of wild-type and mutant fungal strains, using Orbitrap high-resolution mass spectrometry (HRMS) accurate mass data. The suitability of this approach was demonstrated by the confirmation of previously characterised genes involved in the aflatoxin biosynthetic pathway, namely a polyketide synthase (pksA), an oxidoreductase (ordA) and a methyltransferase (omtA) gene. Subsequently, the proposed methodology was applied for the detection and identification of metabolites produced by a yet uncharacterised gene cluster in A. favus, cluster 23. Comparative Orbitrap HRMS analysis of extracts of A. flavus wild-type strain and an over-expression mutant for the transcription factor of gene cluster 23 (lepE) demonstrated that this gene cluster is responsible for the production a set of 2-pyridone derivatives, the leporins. Besides the known derivatives leporin B and leporin B precursor that could be identified by automatic de-replication of the accurate mass data, five other compounds belonging to this class of fungal secondary metabolites were detected and identified for the first time, combining MS and multiple-stage MS data.

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“…Development of a yellowish orange color of the colonies at the reverse side of the plate indicates the presence of Aspergillus flavus whereas green colored colonies indicate the presence of Aspergillus parasiticus [25]. …”

Section: Isolation and Screening Of Aflatoxicogenic Fungi From The Chmentioning

confidence: 99%

Identification of Aflatoxigenic Fungi and Detection of Their Aflatoxin in Red Chilli (<i>Capsicum annuum</i>) Samples Using Direct Cultural Method and HPLC

Hossain¹,

Talukder²,

Afroze³

et al. 2018

AiM

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Aflatoxins are the potential lethal toxin produced by Aspergillus sp. important health hazard throughout the world. In this study, 26 Aspergillus sp. have been isolated from 50 samples of red chilli collected throughout the country. These 26 isolates were grown primarily on agar media to identify the aflatoxin producing species. It is possible to distinguish A. flavus strains from other Aspergillus sp. developing orange colour on the reverse of the plates. The Coconut Cream Agar (CCA) is used to detect aflatoxin producer strains having blue fluorescence when exposed to a UV-light. Several other media were used for morphological characteristics of Aspergillus sp. Out of 26 isolates, four isolates were confirmed as Aspergillus sp. These isolates were subjected to cross contamination with freshly ground, sterile maize and after 15 days of incubation the contaminated maize were analyzed by HPLC and found aflatoxin in each of the sample containing 186 ppb (max.). This study was conducted to assay the ability to produce aflatoxins by the Aspergillus spp. isolated from red chilli (Capsicum annuum L. Solanaceae) available throughout the country. The results found in the experiment are much more behind the acceptable limit according to some international standard. As red chilli is a widely used spice in Bangladesh, the proper controlling measures may be taken for controlling the surveillance of aflatoxinic fungi like as use of bio-pesticides, proper drying method and storage conditions.

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Isolation and structure of red pigments from Aspergillus flavus and related species, grown on a differential medium

Cited by 44 publications

References 4 publications

Fungi associated with the traditional starter cultures used for rice wine in Korea

Fungi associated with the traditional starter cultures used for rice wine in Korea

Use of UHPLC high-resolution Orbitrap mass spectrometry to investigate the genes involved in the production of secondary metabolites inAspergillus flavus

Identification of Aflatoxigenic Fungi and Detection of Their Aflatoxin in Red Chilli (<i>Capsicum annuum</i>) Samples Using Direct Cultural Method and HPLC

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Isolation and structure of red pigments from Aspergillus flavus and related species, grown on a differential medium

Cited by 44 publications

References 4 publications

Fungi associated with the traditional starter cultures used for rice wine in Korea

Fungi associated with the traditional starter cultures used for rice wine in Korea

Use of UHPLC high-resolution Orbitrap mass spectrometry to investigate the genes involved in the production of secondary metabolites inAspergillus flavus

Identification of Aflatoxigenic Fungi and Detection of Their Aflatoxin in Red Chilli (&lt;i&gt;Capsicum annuum&lt;/i&gt;) Samples Using Direct Cultural Method and HPLC

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Identification of Aflatoxigenic Fungi and Detection of Their Aflatoxin in Red Chilli (<i>Capsicum annuum</i>) Samples Using Direct Cultural Method and HPLC