Isolation and Structure of Pompilidotoxins, Novel Peptide Neurotoxins in Solitary Wasp Venoms

Konno, Katsuhiro; Hisada, Miki; Itagaki, Yasuhiro; Naoki, Hideo; Kawai, Nobufumi; Miwa, Akiko; Yasuhara, Tadashi; Takayama, Hiroaki

doi:10.1006/bbrc.1998.9299

Cited by 73 publications

(58 citation statements)

References 15 publications

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“…Considering the insects, the persistent use of insect-based medicines means that substances of therapeutic value not yet known by science may really be present. Pemberton (1999) (Konno et al, 1998). During this survey, a new mast cell degranulating peptide designated as eumenine mastoparan-AF (EMP-AF) has been identified in the venom of the solitary wasp Anterhynchium flavomarginatum micado, the most abundant eumenine wasp in Japan.…”

Section: Pharmacological Importance Of the Animal-derived Folk Medicinesmentioning

confidence: 99%

Implications and applications of folk zootherapy in the state of Bahia, Northeastern Brazil

Neto

2004

Sustainable Development

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This paper deals with the use of 180 animal species as medicinal resources in the state of Bahia, Northeastern Brazil. Data, which come from field works carried out in different indigenous, traditional, rural and urban communities within Bahia State, were gathered through the compilation of information available in scientific articles, monographs, theses, reports and presented papers. The animal-based folk medicines come from millipedes (0.5%), annelids (1.0%), amphibians (1.0%), arachnids (1.0%), echinoderms (1.5%), crustaceans (4.0%), molluscs (6.0%), reptiles (12.0%), birds (13.0%), fishes (17.0%), mammals (20.0%) and insects (23.0%). These bioresources provide about 300 raw materials, which are turned into medicines and prescribed to treat a wide range of locally diagnosed ailments. The result shows how important this ethnozoological phenomenon is, and indicates that traditional knowledge on zootherapy is to be seriously studied in order to lead to the discovery of new sources of drugs. Copyright © 2004 John Wiley & Sons, Ltd and ERP Environment.

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Section: Pharmacological Importance Of the Animal-derived Folk Medicinesmentioning

confidence: 99%

Implications and applications of folk zootherapy in the state of Bahia, Northeastern Brazil

Neto

2004

Sustainable Development

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“…Toxins are classified into five groups on the basis of their discrete binding sites. Recently, a novel neuroactive toxin, > -pompilidotoxin, has been purified from the venom of the spider wasp (15). Using chimeric mutants of NaV1.2 and NaV1.5, the binding site for this toxin has been shown to be the S3 -S4 extracellular loop of domain IV (16).…”

Section: Namentioning

confidence: 99%

Molecular Diversity of Structure and Function of the Voltage-Gated Na+ Channels

Ogata

Ohishi

2002

Japanese Journal of Pharmacology

158

108

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ABSTRACT-A variety of different isoforms of voltage-sensitive Na+ channels have now been identified. The recent three-dimensional analysis of Na + channels has unveiled a unique and unexpected structure of the Na + channel protein. Na + channels can be classified into two categories on the basis of their amino acid sequence, NaV1 isoforms currently comprising nine highly homologous clones and NaX that possesses structure diverging from Na V 1, especially in several critical functional motifs. Although the functional role of Na V 1 isoforms is primarily to form an action potential upstroke in excitable cells, recent biophysical studies indicate that some of the Na V 1 isoforms can also influence subthreshold electrical activity through persistent or resurgent Na + currents. Na V 1.8 and Na V 1.9 contain an amino acid sequence common to tetrodotoxin resistant Na + channels and are localized in peripheral nociceptors. Recent patch-clamp experiments on dorsal root ganglion neurons from Na V 1.8-knock-out mice unveiled an additional tetrodotoxin-resistant Na + current. The demonstration of its dependence on Na V 1.9 provides evidence for a specialized role of Na V 1.9, together with NaV1.8, in pain sensation. Although NaX has not been successfully expressed in an exogenous system, recent investigations using relevant native tissues combined with gene-targeting have disclosed their unique "concentration"-sensitive but not voltage-sensitive roles. In this context, these emerging views of novel functions mediated by different types of Na + channels are reviewed, to give a perspective for future research on the expanding family of Na + channel clones.

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“…Although the peptide that induces a strong stimulatory action on lobster neuromuscular transmission has been identified as a-PMTX, 14 other peptides have yet to be analyzed. The MALDI spectrum of the peptide designated As-183 showed a [M H] ion peak at m/z 1153.8, and its CID/PSD mass spectrum is shown in Fig.…”

Section: Sequence Determination Of Novel Peptides From Anoplius Samarmentioning

confidence: 99%

“…The peak at m/z 827.5 corresponded to the C-terminal rearrangement ion, b H 2 O 16,17 (809.5 18), and m/z 911.5 was y-NH 3 (928. [6][7][8][9][10][11][12][13][14][15][16][17], whereas m/z 884.5 was unidentified. Thus, the partial sequence G-I/ L-L-K/Q-R-I/L in the N-terminal region was determined (Fig 3(a)).…”

Section: Sequence Determination Of Novel Peptides From Anoplius Samarmentioning

confidence: 99%

Advantages of using nested collision induced dissociation/post-source decay with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: sequencing of novel peptides from wasp venom

Hisada

Konno

Itagaki

et al. 2000

Rapid Commun. Mass Spectrom.

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We have examined the applicability of the ‘nested’ collision induced dissociation/post‐source decay (CID/PSD) method to the sequencing of novel peptides from solitary wasps which have neurotoxic venom for paralyzing other insects. The CID/PSD spectrum of a ladder peptide derived from an exopeptidase digest was compared with that of the intact peptide. The mass peaks observed only in the CID/PSD spectrum of a ladder peptide were extracted as C‐terminal fragment ions. Assignment of C‐terminal fragment ions enabled calculation of N‐terminal fragment masses, leading to differentiation between N‐terminal fragment ions and internal fragment ions. This methodology allowed rapid and sensitive identification by removing ambiguity in the assignment of the fragment ions, and proved useful for sequencing unknown peptides, in particular those available as natural products with a limited supply. Copyright © 2000 John Wiley & Sons, Ltd.

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Isolation and Structure of Pompilidotoxins, Novel Peptide Neurotoxins in Solitary Wasp Venoms

Cited by 73 publications

References 15 publications

Implications and applications of folk zootherapy in the state of Bahia, Northeastern Brazil

Implications and applications of folk zootherapy in the state of Bahia, Northeastern Brazil

Molecular Diversity of Structure and Function of the Voltage-Gated Na+ Channels

Advantages of using nested collision induced dissociation/post-source decay with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: sequencing of novel peptides from wasp venom

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