Isolation and Structure of a Novel Indophenol-reducing and 1,1-Diphenyl-2-picrylhydrazyl Radical-scavenging Compound from a Fungus

Abstract

A nove] indophenol-reducing and DPPH radicalscavellging compeumd (1) was iselated from a fungal metabolite. The structure ef 1 was determined by a spectroscopic analysis, and the absolllte configuration of 1 was determined by the modMed Mosher's method. Compound 1 showed slow･reacting DPPH radicalscayenging activity (EDse value of 298 "M sfter 96 hr).

New Potent DPPH Radical Scavengers from a Marine-Derived Actinomycete Strain USF-TC31

“…[1][2][3][4][5][6] Marine-derived microorganisms, especially actinomycetes, produce diverse secondary metabolites which are biologically active and structurally unique compounds. [4][5][6] In the course of our screening program for antioxidants, we have previously reported several new compounds from soil-derived microorganisms [7][8][9][10][11] and fermented foods. 12,13) We are interested in marine natural products as a new source and continue to investigate new antioxidants from the metabolites of marine-derived microorganisms in a manner similar to that reported in our previous studies.…”

Indole Derivatives from a Marine Sponge-Derived Yeast as DPPH Radical Scavengers

“…DPPH radical scavenging assay was performed by the modified method presented previously. − An ethanolic solution of the sample (750 μM, 1 mL) was mixed with a 500 μM DPPH ethanolic solution (0.5 mL) and 0.1 M acetate buffer (pH 5.5, 1 mL). The reaction mixture was left to stand for 48 h, at which time the absorbance of the mixture was measured at 517 nm.…”

“…Therefore, antioxidants are expected to be useful tools to combat and prevent those diseases. 1,2 In our screening program for antioxidants, we have reported several new compounds from soil-derived microorganisms [3][4][5][6][7] and fermented foods 8,9 in the past. We continued to investigate new antioxidants from microorganisms, and recently a yeast strain (USF-HO25) was found as a producer of the DPPH (2,2-diphenyl-1picrylhydrazyl) 10 radical scavenging compounds.…”

Antioxidant and Antimicrobial Phenolic Compounds from Setaria viridis

“…DPPH Assays. DPPH assays were carried out according to the method reported in the literature with minor modifications [11,12]. Briefly, 100 PL of the test samples at different concentrations in EtOH and 100 PL of DPPH (Sigma) in EtOH (200 PL) were added in a 96-well microplate.…”