Isolation and structure elucidation of two new antioxidant flavonoid glycosides and fatty acid composition in Hedysarum carnosum Desf.

Salah, Nizar Ben; Casabianca, Hervé; Essaidi, Ismahen; Chenavas, Sophie; Fildier, Aurélie; Sanglar, Corinne; Jannet, Hichem Ben; Bouzouita, Nabiha

doi:10.1016/j.indcrop.2015.11.057

Cited by 7 publications

(12 citation statements)

References 22 publications

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“…Identification of compounds was performed considering their retention times; UV absorptions and mass spectral data were also compared with those of available standard compounds, as well as with previously identified constituents of Hedysarum spp. And other data reported in the literature [ 20 , 21 , 22 , 26 , 27 , 28 ].…”

Section: Resultsmentioning

confidence: 85%

“…This indicated that the hexose was attached to the aglycone moiety and the two rhamnose units were attached to the hexose rather than the aglycone. These compounds were previously detected in H. coronarium extracts [ 21 ] and also characterized in H. carnosum [ 22 ].…”

Section: Resultsmentioning

confidence: 86%

“…has been performed in order to provide chemotaxonomic evidence for the genus [ 16 ] and to support the pharmacological application of several species within the genus [ 3 , 5 , 17 ]. The phytochemical study led to the identification of several specialized metabolites such as flavonoids (flavones, flavanones, flavanols, isoflavones, pterocarpanes, and chalcones) [ 16 , 18 , 19 , 20 , 21 , 22 , 23 ], xanthones [ 3 ], tannins [ 21 ], coumestans [ 16 , 24 ], benzofurans [ 16 , 20 , 25 ], and oleanane saponins [ 1 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Identification of Specialized Metabolites from Sulla (Hedysarum coronarium L.) Collected in Southern Italy

Tava¹,

Biazzi²,

Ronga

et al. 2021

Molecules

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Sulla (Hedysarum coronarium L.) is a biennal forage legume originated from the Mediterranean basin and used for animal feeding due to its high forage quality and palatability. Several species of Hedysarum have been considered for their nutritional, pharmaceutical, and biological properties, and different applications have been reported, both for human consumption and animal nutrition. Although a systematic investigation of the chemical constituents of Hedysarum spp. has been performed in order to provide chemotaxonomic evidences for the genus and to support the pharmacological application of several species within the genus, few data are available on the chemical constituents of H. coronarium, and only the content of condensed tannins and flavonoids in leaves has been previously reported. In the present paper, results from a detailed chemical analysis of the extracts from the leaves and flowers of H. coronarium grown wild in southern Italy are presented. Identification of the main specialized metabolites within the chemical classes of flavonoids, proanthocyanidins and saponins, is described, including considerations on their content in the two plant organs. Information acquired from this study expands the knowledge on H. coronarium as a source of valuable phytochemicals for different applications in human and animal health and nutrition.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chemical Identification of Specialized Metabolites from Sulla (Hedysarum coronarium L.) Collected in Southern Italy

Tava¹,

Biazzi²,

Ronga

et al. 2021

Molecules

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show abstract

“…At the concentration of 25 µg/mL, compounds 1 and 2 showed the maximum scavenging activities for DPPH radical (81.28 and 83.98%, respectively), which are comparable with those of vitamin C and rutin. The IC 50 of compounds 1 and 2 was 12.67 and 12.30 µg/mL, respectively, which were lower than those of other FG identified before from C. oleifera meal and other plant materials (Ben Salah et al., ; Gao et al., ), indicating the strong antioxidant activities of the two purified compounds. Considering its high contents of these bioactive compounds with strong antioxidant activity, C. oleifera meal can be reused not only for the production of health‐promoting substances, but also as an environment‐friendly natural bioresource, which is supported by the recent finding that antioxidant‐abundant Camellia can serve as a promising electron shuttle for bioelectricity generation in microbial fuel cells (Chen, Liao, Hsu, Tsai, & Hsueh, ).…”

Section: Resultsmentioning

confidence: 67%

Two Kaempferol Glycosides Separated from Camellia Oleifera Meal by High‐Speed Countercurrent Chromatography and Their Possible Application for Antioxidation

Zheng

Chen

et al. 2019

Journal of Food Science

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Recently, kaempferol and its glycosides have attracted considerable attention owing to their potentially health‐benefitting properties including protection against chronic diseases. Here, a microwave‐assisted extraction (MAE) method was developed for the extraction of total flavonoid glycosides (FG) from Camellia oleifera meal, a major agrifood waste largely generated as a byproduct from the Camellia oil processing industry. Compared with traditional extraction methods, MAE enables more efficient extraction of FG. High‐speed countercurrent chromatography was then applied to separate FG from MAE extract, and two major compounds were successfully separated with purities above 90.0% as determined by HPLC. These two compounds were further identified by UV, FT‐IR, ESI‐MS, 1H‐NMR, and 13C‐NMR as kaempferol 3‐O‐[α‐L‐rhamnopyranosyl‐(1→6)‐β‐D‐glucopyranosyl]‐7‐O‐β‐D‐glucopyranoside and kaempferol 3‐O‐[β‐D‐glucopyranosyl‐(1→4)‐α‐L‐rhamnopyranosyl]‐7‐O‐α‐L‐rhamnopyranoside, which were for the first time separated from C. oleifera meal. The results of antioxidant activity assay demonstrated that both compounds had excellent scavenging activity for DPPH radical, and exhibited protective effects against H2O2‐induced oxidative damage of vascular endothelial cells. The findings of this work suggest the possibility of employing C. oleifera meal as an attractive source of health‐promoting compounds, and at the same time facilitate its high‐value reuse and reduction of environmental burden.

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“…Fabaceae) является ценным лекарственным растением [1]. Для выделения биологически активных веществ (БАВ) из растений данного рода широко используются различные хроматографические методы [1][2][3]. Метод жидкостной колоночной хроматографии успешно применяется как в России, так и в зарубежных странах.…”

Section: Introductionunclassified

Chromatography of Hedysarum extracts on modified Sepharose CL sorbents

Fedorova

Сухих

Суслов

et al. 2019

Fundamentalʹnaâ i kliničeskaâ medicina

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Aim. To study the chromatographic efficiency of modified Sepharose CL-4B sorbents for separation, purification, and preparation of major components from Hedysarum theinum Krasnob. and Hedysarum neglectum Ledeb. root extracts.Materials and Methods. Sorption capacity of the native and modified sepharose was evaluated in both isocratic and gradient elution modes. 4-pyridinecarboxylic acid derivative [(4-hydroxy-3-methoxyphenyl)methylene]hydrazide (ftivazide) was used as a sorbent modifier ligand. We studied chromatographic properties of Sepharose CL-4B, Sepharose CL-6B, and azoepoxyadsorbent Sepharose CL-4B-DEP-p-nitrobenzohydrazide-ftivazide, a chemically modified analogue of Sepharose CL-4B.Results. Sepharose CL-4B and Sepharose CL-4B-DEP-p-nitrobenzohydrazide-ftivazide were found efficient carriers for liquid column chromatography of water-ethanol Hedysarum extracts. Complete elution of bioactive substances has been achieved exclusively by using sodium tetraborate as an eluent. We were able to identify flavonoids and tannins of different condensation in chromatographic fractions.Conclusion. Comparative chromatography of bioactive substances from Hedysarum root extracts is more efficient if employing Sepharose CL-4B instead of Sepharose CL-6B as a sorbent. Sepharose CL-4B-DEP-p-nitrobenzohydrazide-ftivazide demonstrates excellent separation capacity for tannins and flavonoids.

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Isolation and structure elucidation of two new antioxidant flavonoid glycosides and fatty acid composition in Hedysarum carnosum Desf.

Cited by 7 publications

References 22 publications

Chemical Identification of Specialized Metabolites from Sulla (Hedysarum coronarium L.) Collected in Southern Italy

Chemical Identification of Specialized Metabolites from Sulla (Hedysarum coronarium L.) Collected in Southern Italy

Two Kaempferol Glycosides Separated from Camellia Oleifera Meal by High‐Speed Countercurrent Chromatography and Their Possible Application for Antioxidation

Chromatography of Hedysarum extracts on modified Sepharose CL sorbents

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