Isolation and structural studies of heparan sulfates and chondroitin sulfates from three species of molluscs.

Nader, Helena B.; Ferreira, Thais M.P.C.; Paiva, J F; Medeiros, Maria G.L.; Jerônimo, Selma M. B.; Paiva, Vania M.P.; Dietrich, Carl P.

doi:10.1016/s0021-9258(17)43424-7

Cited by 89 publications

(9 citation statements)

References 21 publications

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“…Marine animals are known to contain sulfated polysaccharides with unique structural features (Cassaro and Dietrich 1977;Nader et al 1984;Medeiros et al 2000;Volpi and Maccari 2009;Kozlowski et al 2011). Sulfated GAGs have important roles in vertebrate biology, and have been implicated in diverse Fig.…”

Section: Discussionmentioning

confidence: 99%

Brittlestars contain highly sulfated chondroitin sulfates/dermatan sulfates that promote fibroblast growth factor 2-induced cell signaling

et al. 2013

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Glycosaminoglycans (GAGs) isolated from brittlestars, Echinodermata class Ophiuroidea, were characterized, as part of attempts to understand the evolutionary development of these polysaccharides. A population of chondroitin sulfate/dermatan sulfate (CS/DS) chains with a high overall degree of sulfation and hexuronate epimerization was the major GAG found, whereas heparan sulfate (HS) was below detection level. Enzymatic digestion with different chondroitin lyases revealed exceptionally high proportions of di- and trisulfated CS/DS disaccharides. The latter unit appears much more abundant in one of four individual species of brittlestars, Amphiura filiformis, than reported earlier in other marine invertebrates. The brittlestar CS/DS was further shown to bind to growth factors such as fibroblast growth factor 2 and to promote FGF-stimulated cell signaling in GAG-deficient cell lines in a manner similar to that of heparin. These findings point to a potential biological role for the highly sulfated invertebrate GAGs, similar to those ascribed to HS in vertebrates.

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Section: Discussionmentioning

confidence: 99%

Brittlestars contain highly sulfated chondroitin sulfates/dermatan sulfates that promote fibroblast growth factor 2-induced cell signaling

et al. 2013

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“…This polysaccharide shows, in particular, a low charge density due to the presence of 21% nonsulfated chondroitin and approximately 26% nonsulfated disaccharides inside the CS chains probably caused by an enzymatic desulfation in the mollusc. Low-sulfated CS molecules have been purified and characterized from other species of Mollusca gastropoda 12,33 and in Drosophila melanogaster and Caenorhabditis elegans. 34 Pharmaceutical heparin is typically prepared from bovine or porcine intestinal mucosa or beef lung.…”

Section: Discussionmentioning

confidence: 99%

“…[6][7][8] The presence of sulfated GAGs in some taxa of invertebrates is now well-documented. [9][10][11][12][13][14][15][16][17][18] A comprehensive survey of different classes of invertebrates has shown that CS/DS, HS-like, and/or heparin-like compounds are present in many species. 16 Previous studies have also shown that heparin is present in several species of molluscs.…”

Section: Introductionmentioning

confidence: 99%

Glycosaminoglycan Composition of the Large Freshwater Mollusc Bivalve Anodonta anodonta

Volpi

Maccari

2005

Biomacromolecules

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In this paper, glycosaminoglycans from the body of the large freshwater mollusc bivalve Anodonta anodonta were recovered at about 0.6 mg/g of dry tissue, composed of chondroitin sulfate (approximately 38%), nonsulfated chondroitin (about 21%), and heparin (41%). This last polysaccharide was found to consist of a large percentage (approximately 88%) of a fast-moving species possessing a lower molecular mass and sulfate group amount and about 12% of a more sulfated, slow-moving component having a greater molecular mass. The chondroitin sulfate was composed of approximately 28% of the 6-sulfated disaccharide, 46% of the 4-sulfated disaccharide, and about 26% of the nonsulfated disaccharide, with a charge density value of 0.74. Heparin was subjected to the oligosaccharide mapping after treatment with heparinase and then separation of the resulting unsaturated oligosaccharides by SAX-HPLC. A heparin sample from Anodonta anodonta showed a degree of sulfation similar to that of bovine mucosal heparin because of the presence of approximately the same mol % of the trisulfated disaccharide (DeltaUA2S(1-->4)-alpha-D-GlcN2S6S), a slight modification of the other oligosaccharides, and a significant increase of the disaccharide bearing the sulfate group in position 3 of the N-sulfoglucosamine 6-sulfate (-->4)-beta-D-GlcA(1-->4)-alpha-D-GlcN2S3S6S(1-->) part of the ATIII-binding region. However, the anticoagulant activity of mollusc heparin was quite similar to that of pharmaceutical grade heparin. The data obtained again emphasize the heterogeneity of GAGs from molluscs.

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“…Bovine lung heparin (Hep, 9.5-12 kDa) was obtained from Opocrin Research Laboratory (Corlo, Mo, Italy), shark chondroitin 6-sulfate (C6S, 40-60 kDa) and bovine intestinal dermatan sulfate (DS, 21.5 kDa) from Seikagaku Kogyo Co. (Tokyo, Japan), and bovine chondroitin 4-sulfate (C4S, 27 kDa) from Sigma-Aldrich (St. Louis, Mo, USA). Bovine cartilage keratan sulfate (KS) [17], bovine lung heparan sulfate (HS, 30-40 kDa) [18], and elastase inhibitor (CeEI) from Caesalpinia echinata seeds (unpublished data) were purified in our laboratory.…”

Section: Methodsmentioning

confidence: 99%

Glycosaminoglycans Modify Elastase ActionIn Vitroand Enhance Elastase-Induced Cell Death in Cultured Fibroblasts

et al. 2012

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Human neutrophil elastase (HNE) has been shown to be involved on death of different cell types, including epithelial lung cells, which is related to several pulmonary diseases. Since HNE activity may be influenced by extracellular matrix (ECM) molecules such as glycosaminoglycans (GAGs), and fibroblasts are the most common ECM-producing cells of lung connective tissue, the aim of this work was to verify if HNE can induce fibroblast death and to study the enzyme modulation by GAGs. HNE-like activity was mimicked by using human neutrophils conditioned medium (NCM). Heparan sulfate and chondroitin 6-sulfate reduce the enzyme activity and modify its secondary structure. NCM reduced cell viability, and this effect was higher in the presence of those GAGs. NCM also increased DNA fragmentation, suggesting the occurrence of apoptosis, but without influence of GAGs. These results can contribute to the understanding of HNE modulation in physio- and pathological processes where this enzyme is involved.

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Isolation and structural studies of heparan sulfates and chondroitin sulfates from three species of molluscs.

Cited by 89 publications

References 21 publications

Brittlestars contain highly sulfated chondroitin sulfates/dermatan sulfates that promote fibroblast growth factor 2-induced cell signaling

Brittlestars contain highly sulfated chondroitin sulfates/dermatan sulfates that promote fibroblast growth factor 2-induced cell signaling

Glycosaminoglycan Composition of the Large Freshwater Mollusc Bivalve Anodonta anodonta

Glycosaminoglycans Modify Elastase ActionIn Vitroand Enhance Elastase-Induced Cell Death in Cultured Fibroblasts

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