Isolation and structural identification of a novel fructan from Radix Codonopsis

Li, Jiankuan; Yan, Wang; Zhang, Xia; Cao, Lingya; Ji, Jiaojiao; Zheng, Qinghong; Gao, Jianping

doi:10.1080/07328303.2020.1772278

Cited by 11 publications

(6 citation statements)

References 21 publications

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“…Additionally, the branch of (2→6)-β-D-Fru f was demonstrated according to the HSQC and HMBC spectra analysis. In view of the above results, the CPPs can be identified as a glucofructan, and the possible structural formula is shown in Figure 5 D, which is quite different from the other inulin-type fructan isolated from C. pilosula in previous literatures [ 42 , 43 ]. These diversities were probably caused mainly by diverse extraction and purification methods.…”

Section: Resultsmentioning

confidence: 72%

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Jiao

et al. 2022

Nutrients

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In recent years, multiple edible polysaccharides from Codonopsis pilosula were mainly isolated with high average molecular weights and exhibited various bioactivities, but it was proven that low-molecular-weight polysaccharides could exert stronger activities due to the superior water solubility and permeability. In the present study, the water-soluble polysaccharide C. pilosula with low molecular weight was isolated under ultrasonic assistance at 30 °C, the extraction process was optimized via response surface method (RSM), and the structure and immunoregulatory activity were further investigated. The maximum yield (4.86%) for crude polysaccharides (cCPPs) was obtained under following parameters: ultrasonic power of 370 W, liquid/material ratio of 33 mL/g, ultrasonic time of 81 min. Subsequently, the cCPPs were further purified through dialysis and Sephadex G-25 column to acquire purified polysaccharide (CPPs). Structural analysis indicated that CPPs was a glucofructan (average molecular weight of 4.23 × 103 Da) with (2→1)-β-D-Fruf and (1→)-α-D-Glcp as the backbone branched by (2→6)-β-D-Fruf. Additionally, CPPs could enhance immunoregulatory function by stimulating NO production and cytokine (IL-6 and TNF-α) secretion of RAW264.7 macrophages dose-dependently, which presented no cytotoxic effects. These data suggest that CPPs have the potential to be used as a nutritional dietary compound and natural immunostimulant supplement in the food industry.

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Section: Resultsmentioning

confidence: 72%

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Jiao

et al. 2022

Nutrients

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“…Finally, the most recently described fructan possesses a novel β(2→3) linkage and high molecular weight (1.70 × 10 3 kDa), and it was reported in the Radix Codonopsis. The structure of this fructan was confirmed by one dimension and bidimensional nuclear magnetic resonance, but the new class of fructan has not been placed in any of the abovementioned categories [41]. Table 1 shows a general fructan classification.…”

Section: Fructan Diversitymentioning

confidence: 92%

Fructooligosaccharides (FOS) Production by Microorganisms with Fructosyltransferase Activity

Belmonte-Izquierdo,

Salomé-Abarca,

González-Hernández

et al. 2023

Fermentation

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Fructans are fructose-based polymers, defined as fructooligosaccharides (FOS), when they possess a short chain. These molecules are highly appreciated in the food and pharmaceutical international market and have an increasing demand worldwide, mainly for their prebiotic activity and, therefore, for all their health benefits to those who consume them constantly. Thus, new natural or alternative FOS production systems of industrial scale are needed. In this regard, microorganisms (prokaryotes and eukaryotes) have the potential to produce them through a wide and diverse number of enzymes with fructosyltransferase activity, which add a fructosyl group to sucrose or FOS molecules to elongate their chain. Microbial fructosyltransferases are preferred in the industry because of their high FOS production yields. Some of these enzymes include levansucrases, inulosucrases, and β-fructofuranosidases obtained and used through biotechnological tools to enhance their fructosyltransferase activity. In addition, characterizing new microorganisms with fructosyltransferase activity and modifying them could help to increase the production of FOS with a specific degree of polymerization and reduce the FOS production time, thus easing FOS obtention. Therefore, the aim of this review is to compile, discuss, and propose new perspectives about the microbial potential for FOS production through enzymes with fructosyltransferase activity and describe the modulation of FOS production yields by exogenous stimuli and endogenous modifications.

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“…The chemical structure of an inulin-type fructan purified from Platycodon grandiflorum roots was shown by 1D- and 2D-NMR (including COSY, TOCSY, HSQC, and HMBC) to include a backbone mainly composed of (2→1)-linked β-D-fructofuranose units with a terminal α-D-glucopyranose [ 53 ]. A novel fructan from Radix Codonopsis was shown, based on data obtained from HMBC and COSY spectra, to contain α-D-fructofuranosyl-(2→3)-β-D-fructofuranosyl linkages [ 63 ].…”

Section: Characterisationmentioning

confidence: 99%

Preparation, Structural Characterisation, and Bioactivities of Fructans: A Review

Cheong

2023

Molecules

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Polysaccharides are important components of higher plants and have attracted increasing attention due to their many nutraceutical benefits in humans. Fructans, heterogeneous fructose polymers that serve as storage carbohydrates in various plants, represent one of the most important types of natural polysaccharides. Fructans have various physiological and therapeutic effects, which are beneficial to health, and have the ability to prevent or treat various diseases, allowing their wide use in the food, nutraceutical, and pharmaceutical industries. This article reviews the occurrence, metabolism, preparation, characterisation, analysis, and bioactivity of fructans. Further, their molecular weight, monosaccharide composition, linkages, and structural determination are described. Taken together, this review provides a theoretical foundation for further research into the structure–function relationships of fructans, as well as valuable new information and directions for further research and application of fructans in functional foods.

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Isolation and structural identification of a novel fructan from Radix Codonopsis

Cited by 11 publications

References 21 publications

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Ultrasonic-Assisted Extraction of Codonopsis pilosula Glucofructan: Optimization, Structure, and Immunoregulatory Activity

Fructooligosaccharides (FOS) Production by Microorganisms with Fructosyltransferase Activity

Preparation, Structural Characterisation, and Bioactivities of Fructans: A Review

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